A5036401922- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Ovarian function and disorders
- Pluripotent Stem Cells Research
- Effects of Environmental Stressors on Livestock
- Renal and related cancers
- Reproductive System and Pregnancy
- MicroRNA in disease regulation
- Ruminant Nutrition and Digestive Physiology
- Rabbits: Nutrition, Reproduction, Health
- Animal Diversity and Health Studies
- Bioenergy crop production and management
- Selenium in Biological Systems
- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
- Birth, Development, and Health
- Bee Products Chemical Analysis
- Adipose Tissue and Metabolism
- Circular RNAs in diseases
- Livestock Management and Performance Improvement
- Silymarin and Mushroom Poisoning
Cairo University
2015-2025
University of Göttingen
2023-2025
GTx (United States)
2024
Gyeongsang National University
2013-2022
Natural Resources Institute Finland
2015
University of Bonn
2007-2011
MicroRNAs are the major class of gene-regulating molecules playing diverse roles through sequence complementarity to target mRNAs at post-transcriptional level. Tightly regulated expression and interaction a multitude genes for ovarian folliculogenesis could be by these miRNAs. Identification them is first step towards understanding miRNA-guided gene regulation in different biological functions. Despite increasing efforts miRNAs identification across various species tissue types, little...
Aberrant gene expression in the uterine endometrium and embryo has been major causes of pregnancy failure cattle. However, selecting cows having adequate endometrial receptivity embryos better developmental competence based on pattern a greater challenge. To investigate whether pretransfer direct relation with upcoming success, we performed global transcriptome analysis using biopsy technology outcome information. For this, samples were collected from Simmental heifers at day 7 14 estrous...
Oocyte selection based on glucose-6-phosphate dehydrogenase (G6PDH) activity has been successfully used to differentiate between competent and incompetent bovine oocytes. However, the intrinsic molecular subcellular characteristics of these oocytes have not yet investigated. Here, we aim identify functional markers associated with oocyte developmental potential when selected G6PDH activity. Immature compact cumulus–oocyte complexes were stained brilliant cresyl blue (BCB) for 90 min. Based...
Although it is well established that maturation conditions have a clear influence on oocyte developmental competence, not known whether this could be due to downstream effects of perturbation the transcript profile oocyte’s adjacent cumulus cells. Therefore, aim present study was compare profiles cells derived from cumulus–oocyte complexes (COCs) matured in vitro or vivo. Using previously validated combined synchronisation and superstimulation protocol, COCs were recovered beef heifer...
This study was conducted to investigate the gene expression profile of in vivo -derived bovine embryo biopsies based on pregnancy outcomes after transferring recipients. For this, 30–40% embryos were taken from grade I blastocysts (International Embryo Transfer Society Manual) and remaining 60–70% intact transferred Frozen pooled into three distinct groups outcome corresponding parts, namely those resulting no (NP), loss (PL), calf delivery (CD). Array analysis revealed a total 41 43 genes...
In mammals, the reproductive tract plays a crucial role in success of early events and provides an optimal microenvironment for embryonic development. However, changes environment associated with controlled ovarian hyperstimulation influence on embryo transcriptome profile have not been investigated. Therefore, we investigated differences development rate bovine blastocysts developing unstimulated or superovulated heifers. Nineteen Simmental heifers were synchronized, artificially...
The highly prolific breeds of domestic sheep (Ovis aries) are globally valuable genetic resources for industry. Genetic, nutritional and other environmental factors affect prolificacy traits in sheep. To improve our knowledge the traits, we conducted mRNA-miRNA integrated profiling ovarian tissues from two pure with large (Finnsheep) vs. small (Texel) litter sizes their F1 crosses, half which were fed a flushing diet. Among samples, 16,402 genes (60.6% known ovine genes) expressed, 79 novel...
The Well of the (WOW) system has been developed to culture embryos in small groups or track development single embryos. In present study, we aimed examine effects microenvironment provided by WOW and embryo density on developmental rates, quality preimplantative gene expression profile resulting Embryos cultured a group 16 reached blastocyst stage at significantly lower level than zygotes 50 (22.2 vs 30.3%), whereas were able compensate against low densities, reaching rate as high (31.3...
The aim of the present study was to analyse effect subclinical endometritis on endometrial and embryonic gene expression. A total 49 cows at either Day 0 or 7 oestrous cycle (62-83 days post partum) following superovulation were classified as having (SE-0, SE-7) a healthy endometrium (HE-0, HE-7) basis cytological evaluation. Endometrial samples associated embryos subjected global transcriptome analysis using Bovine GeneChip (Affymetrix, Santa Clara, CA, USA) aberrant transcript profiles...
Abstract Sustainable agriculture approaches necessitate a concerted effort from researchers to establish paths that meet global population needs without compromising environmental resources. Goats are unique among ruminants because of their ability adapt some the harshest environments around world. Growth Hormone (GH) gene is major regulator muscle mass growth. Therefore, our study aims analyze whole sequence GH in order identify various single nucleotide polymorphisms (SNPs) Damascus and...
In vitro culture impairs mitochondrial metabolism of IVP derived bovine embryos resulting in accumulation reactive oxygen species. Recently, the antioxidant Mito-TEMPO has attracted attention due to its capability accumulate within mitochondria. order investigate potential improve quality embryos, this study analyzed developmental stage specific effect on capacity, ROS balance, expression outline antioxidative genes and cryo-resilience blastocysts. three subsequent experiments (1 μM) was...
Zygotic genome activation (ZGA) is a critical biological step in mammalian early embryo development. However, ZGA initiation sheep and the related sophisticated RNA metabolism remains largely unknown. Here, we observed extensive alterations gene expression DNA methylation patterns, along with elevated levels of polymerase II (RNAPII) its phosphorylation at serine 2 (RNAPII-Ser2P) 16-cell stage. Moreover, embryos were blocked stage when treated α-Amanitin, indicating that initiated vitro...
Abstract In dairy cows, detrimental effects of global warming and intensive genetic selection for high milk yield on reproductive performance have become increasingly relevant in cooler regions. Based the current knowledge we hypothesized that elevated temperature during oocyte maturation affects mitochondria endoplasmic reticulum parallel with mitochondrial dysfunction representing mechanistic link between ROS ER Stress. To proof that, present study aimed to uncover consequences heat stress...
Abstract Climate change is impacting the global spread of infectious diseases, altering pathogen distribution and transmission, threatening human animal health. This study investigates presence potential pathogens in blood within unmapped reads obtained from whole-genome sequencing data various cattle breeds across geographically diverse regions, including South Africa, Uganda, Egypt, Portugal, The Netherlands, Finland. Unmapped were extracted, assembled into contigs, subjected to taxonomic...
Abstract Background Oocyte developmental competence is highly affected by the phase of ovarian follicular wave. Previous studies have shown that oocytes from subordinate follicles recovered at growth (day 3 after estrus) are developmentally more competent than those dominance 7 estrus). However, molecular mechanisms associated with these differences not well elucidated. Therefore, objective this study was to investigate transcript abundance bovine retrieved small and phases first wave...
Contents The current study investigated the possibility of using AMH concentration as a predictor ability Korean Hanwoo cows to produce cumulus‐oocyte complexes, embryos that survive after transfer well pregnancy outcome surrogates. Eight sessions ovum pick‐up ( OPU ) were performed with 19 donor at an interval 3–4 days. Antral follicle count AFC ), oocyte quality and in vitro embryo development recorded for each cow. Embryos produced from different profiles transferred into recipients (n =...