A5067755874- Cancer-related molecular mechanisms research
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Breast Cancer Treatment Studies
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Circular RNAs in diseases
- Genetic and phenotypic traits in livestock
- RNA Research and Splicing
- Cancer-related gene regulation
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- interferon and immune responses
- Radiomics and Machine Learning in Medical Imaging
- Molecular Biology Techniques and Applications
- Sperm and Testicular Function
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Clinical Nutrition and Gastroenterology
- Soybean genetics and cultivation
- TGF-β signaling in diseases
- Kruppel-like factors research
Nanjing Agricultural University
2016-2025
Qingdao University of Science and Technology
2025
Affiliated Hospital of Hebei University
2014-2024
Hebei University
2024
Hunan Agricultural University
2024
Army Medical University
2014-2024
Second Affiliated Hospital of Chongqing Medical University
2024
Dalian Medical University
2024
Chongqing Medical University
2024
ZheJiang Academy of Agricultural Sciences
2024
Mitophagy, as an evolutionarily conserved cellular process, plays a crucial role in preserving metabolism and physiology. Various microenvironment alterations assigned to mitophagy including pH, polarity, deregulated biomarkers are increasingly understood. However, mitophagy-specific viscosity dynamic live cells remains mystery needs be explored. Here, water-soluble mitochondria-targetable molecular rotor, ethyl-4-[3,6-bis(1-methyl-4-vinylpyridium iodine)-9H-carbazol-9-yl)] butanoate (BMVC),...
Reproductive ability, especially prolificacy, impacts sheep profitability. Hu sheep, a unique Chinese breed, is recognized for its high prolificacy (HP), early sexual maturity, and year-round estrus. However, little known about the molecular mechanisms underlying HP in sheep. To explore potential mRNAs long non-coding RNAs (lncRNAs) involved we performed an ovarian genome-wide analysis of lncRNAs during follicular stage using (litter size = 3; three consecutive lambings) low (LP, litter 1;...
Long noncoding RNAs (LncRNAs) have been identified as important regulators of testis development; however, their expression patterns and roles in sheep are not yet clear. Thus, we used stranded specific RNA-seq to profile the transcriptome (lncRNAs mRNAs) premature mature sheep. Hormone levels index were examined, histological analyses performed at five stages development, 5-day-old (D5), 3-month-old (3M), 6-month-old (6M), 9-month-old (9M), 2-year-old (2Y), results which indicate a...
Long non-coding RNA (LncRNA) have been identified as important regulators in the hypothalamic-pituitary-ovarian axis associated with sheep prolificacy. However, their expression pattern and potential roles pituitary are yet unclear. To explore mRNAs lncRNAs that regulate of genes involved prolificacy, we used stranded specific RNA-seq to profile transcriptome (lncRNA mRNA) high prolificacy (genotype FecB BB, litter size = 3; H) low B+; 1; L). Our results showed 57 differentially expressed...
Chronic obstructive pulmonary disease (COPD) is a progressive lung that primarily caused by cigarette smoke (CS)-induced chronic inflammation. In this study, we investigated the function and mechanism of action long non-coding RNA (lncRNA) taurine-up-regulated gene 1 (TUG1) in CS-induced COPD. We found expression TUG1 was significantly higher sputum cells tissues patients with COPD as compared to non-smokers, negatively correlated percentage predicted forced expiratory volume second....
Spermatogenesis is an important biological process in male reproduction. The interaction between germ cells and somatic during spermatogenesis, necessary for reproductive activities. This cellular heterogeneity has made it difficult to profile distinct cell types at different stages of development. Here, we present the first comprehensive, unbiased single-cell transcriptomic study sheep spermatogenesis using 10× genomics single sequencing (scRNA-seq). We collected scRNA-seq data from 11 772...
Abstract Dual blocker therapy (DBT) has the enhanced antitumor benefits than monotherapy. Yet, few effective biomarkers are developed to monitor response. Herein, we investigate DBT longitudinal plasma proteome profiling including 113 samples from 22 patients who received anti-PD1 and anti-CTLA4 therapy. The results show immune response cholesterol metabolism upregulated after first cycle. Notably, is activated in disease non-progressive group (DNP) during Correspondingly, clinical indicator...
Epitope-based vaccine is a promising strategy for therapeutic vaccination against Helicobacter pylori (H. pylori) infection. A multivalent subunit containing various antigens from H. superior to univalent vaccine. However, whether epitope-based in pylori, remains unclear. In this study, named CWAE urease, neutrophil-activating protein (NAP), heat shock 60 (HSP60) and adhesin (HpaA) was constructed based on mucosal adjuvant cholera toxin B (CTB), Th1-type NAP, multiple copies of selected Th...
Ovulation rate and litter size are important reproductive traits in sheep with high economic value. Recent work has revealed a potential link between DNA methylation prolificacy. However, genome-wide study that sought to identify sites involved prolificacy indicated it is still unknown. Here, we aimed investigate the profiles of Hu ovaries by comparing high-prolificacy group (HP, three for at least 2 consecutive lambings) low (LP, one using deep whole-genome bisulfite sequencing (WGBS)....
Objective: To investigate how microRNA-190 (miR-190) regulates FOXP2 genes in gastric cancer (GC) cell line SGC7901. Methods: We identified that miR-190 could target by using dual luciferase enzyme assay. Precursor fragment transfection of was performed with GC SGC7901 and human mucosal GES-1. expression detected reverse transcription-polymerase chain reaction (RT-PCR) protein measured Western blotting. Results: FOXP2-3'-untranslated region (UTR) group significantly decreased as compared...
Ki67 is a commonly used marker of cancer cell proliferation, and has significant prognostic value in breast cancer. In spite its clinical importance, assessment remains challenge, as current manual scoring methods have high inter- intra-user variability. A major reason for this variability selection bias, that different observers will score regions the same tumor. Here, we developed an automated method eliminates by using whole-slide analysis to identify tumor with highest proliferative...
Nasal-type natural killer/T-cell lymphoma (NKTCL) is an aggressive malignancy with poor survival outcomes that relatively resistant to chemotherapy. N6-Methyladenosine (m6A) modification, the most prevalent modification of eukaryotic messenger RNA, involved in progression various tumors. However, it unclear whether has a physiological role NKTCL development. To address this question, we probed its function and molecular mechanisms NKTCL. Initially, demonstrated Wilms' tumor 1-associated...
Zygotic genome activation (ZGA) is a critical biological step in mammalian early embryo development. However, ZGA initiation sheep and the related sophisticated RNA metabolism remains largely unknown. Here, we observed extensive alterations gene expression DNA methylation patterns, along with elevated levels of polymerase II (RNAPII) its phosphorylation at serine 2 (RNAPII-Ser2P) 16-cell stage. Moreover, embryos were blocked stage when treated α-Amanitin, indicating that initiated vitro...
Abstract Spermatogenesis can be affected by nutrition, which operates through normal physiological processes changing the testicular mass and hormone levels profoundly. However, little is known regarding how testis development regulated long noncoding RNA (lncRNA). In this study, we investigated effects of high-grain (HG) feeding on during sexual maturation mediated lncRNA. The HG diet group showed an increase in growth (GH), insulin-like factor-1 (IGF-1) testosterone (T) levels, number...
As an important type of noncoding RNA molecules, long non-coding RNAs (lncRNAs) act as versatile players in various biological processes. However, little is known about lncRNA regulators during sheep muscle growth. To explore functional lncRNAs growth, we systematically investigated using strand-specific Ribo-Zero sequencing at three key developmental stages Hu sheep. A total 6924 were obtained, and the differentially expressed genes screened from (control vs. experiment) fetus lamb, lamb...
Abstract The factor that binds to the inducer of short transcripts‐1 (FBI-1) is a transcription suppressor and an important proto‐oncogene plays multiple roles in carcinogenesis therapeutic resistance. In present work, our results indicated FBI-1 enhanced resistance triple-negative breast cancer (TNBC) cells chemotherapeutic agents by repressing expression micoRNA-30c targeting pregnane X receptor (PXR). was positively related PXR its downstream drug resistance-related genes TNBC tissues....
Mitotic count, PhH3, and MIB-1 are used as measures of the proportion proliferating malignant cells in surgical pathology. They highlight different stages cell cycle, but little is known about how this affects their counts. This study assesses strength correlations attempts to determine relationship between them. Proliferation counts for forty-nine consecutive cases invasive breast carcinomas were analyzed, with same tumor area on each stain counted using digital image analysis. The...