A5076762332- Neuropeptides and Animal Physiology
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Neuroendocrine regulation and behavior
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Nutrition and Health
- Coccidia and coccidiosis research
- Reproductive Physiology in Livestock
- Agriculture and Biological Studies
- Reproductive biology and impacts on aquatic species
- Botanical Research and Chemistry
- Glycosylation and Glycoproteins Research
- Potato Plant Research
- Transgenic Plants and Applications
- Animal health and immunology
- Plant Reproductive Biology
- Food Industry and Aquatic Biology
- Agricultural Productivity and Crop Improvement
- Hair Growth and Disorders
- Animal Genetics and Reproduction
- T-cell and Retrovirus Studies
- Hypothalamic control of reproductive hormones
- Herpesvirus Infections and Treatments
- Soybean genetics and cultivation
- Rabbits: Nutrition, Reproduction, Health
University of Bari Aldo Moro
2003-2014
Ludwig-Maximilians-Universität München
2004
University of Veterinary Medicine Vienna
2004
Agrobiogen (Germany)
2004
Complex recombinant antibody fragments for modulation of immune function such as tumor cell destruction have emerged at a rapid pace and diverse anticancer strategies are being developed to benefit patients. Despite improvements in molecule design expression systems, the quantity stability, e.g., single-chain antibodies produced culture, is often insufficient treatment human disease, costs scale-up, labor, fermentation facilities prohibitive. The ability yield mg/ml levels scale-up...
The aim of the present study was to determine relationship progesterone (P4), bovine pregnancy-associated glycoprotein-1 (bPAG-1) and nitric oxide (NO) levels with late embryonic (LEM; day 28 42) early fetal mortalities (EFM; > 42 56) in dairy cows. Transrectal ultrasonography (6–8 MHz) performed 100 Holstein-Friesian cows at days 28, 56 after artificial insemination (AI; 0) diagnose pregnancy monitor fate embryo. After ultrasound scanning each cow, a milk sample collected for assessment P4...
The development of fertilizing ability in sperm cells is associated with changes the plasma membrane. However, to date exact nature sequentially activated primary receptors and channels signal transduction pathways derived from these remains elusive. We analyzed expression localization μ-opioid receptossr equine spermatozoa. A transcript corresponding third extracellular loop that selectively binds μ agonists was amplified, sequenced compared known sequences humans, rats cattle....
Abstract Here we characterize the antitumor activity of a recombinant bispecific single‐chain antibody isolated from serum cloned transgenic cows. The antibody, termed r28M, is directed to melanoma‐associated proteoglycan, also expressed on glioblastoma cells, and human CD28. Bound tumor r28M induced exceedingly efficient supraagonistic T‐cell activation via CD28 molecule without an additional stimulus TCR/CD3 complex. In vitro , T cells NK contributed cell killing after r28M‐mediated...
The presence of the mu-opioid receptor and type glycosylation in third extra-cellular loop this was investigated isthmus mare oviduct during oestrus by means immunoblotting immunohistochemistry combined with enzymatic (N-glycosidase F O-glycosidase) chemical (beta-elimination) treatments. Immunoblotting analysis showed that consists two peptides molecular weights around 65 50 kDa. After N-deglycosylation N-glycosidase an additional immunoreactive peptide observed at 30 KDa. cleavage...
Endogenous opioid peptides (EOP), through G-protein-coupled receptors, control metabolism and many physiological pathological conditions. Once EOP are linked to their above all µ-opioid receptor (MOR), a block of the Ca2+ channel occurs (Sciorsci et al. 2000 Immunopharm. Immunotox. 22, 575–626). The disruption homeostasis interferes with Ca2+-mediated/dependent actions. Our previous studies demonstrated presence MOR in human, bovine, equine oocytes, sperm cells several species (equine,...
Abstract The purposes of the present study were to examine effect naloxone, a mu‐opioid receptor ( MOR ) antagonist, on porcine oocyte maturation and embryo development. gene was expressed in germinal vesicle GV metaphase II M ‐ oocytes, one‐, four‐cell stage embryos blastocysts. In blastocysts, mainly inner cell mass ICM cells. Supplementation 10 −8 mol/L naloxone vitro IVM medium increased rate P < 0.05). However, −4 reduced 0.05) compared with control. presence during had no effects...
Embryonic stem cells can become any tissue in the body, excluding a placenta. Growth factors, hormones, and neurotransmitters have been implicated regulation of their fate. Because various neural precursors express functional neurotransmitter receptors, as G-protein-coupled it is anticipated that they are involved cell fate decisions. Moreover, high level endogenous opioids linked to receptor above all μ opioid receptors (MOR) has shown interfere with normal calcium metabolism activity...