A5016780517- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Advanced Chemical Sensor Technologies
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Ion Channels and Receptors
- Neuroscience and Neuropharmacology Research
- RNA and protein synthesis mechanisms
- Cardiac electrophysiology and arrhythmias
- Insect Utilization and Effects
- Fermentation and Sensory Analysis
- Mast cells and histamine
- Venomous Animal Envenomation and Studies
- Tea Polyphenols and Effects
- Circadian rhythm and melatonin
- Polyamine Metabolism and Applications
- Magnesium in Health and Disease
- Essential Oils and Antimicrobial Activity
- Neuroendocrine regulation and behavior
- Nutrition, Genetics, and Disease
- Calcium signaling and nucleotide metabolism
- Plant Reproductive Biology
- Regulation of Appetite and Obesity
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2015-2024
Technical University of Munich
2018
Leibniz Institute for Neurobiology
2014
German Institute of Human Nutrition
2002-2008
Johns Hopkins University
1998
Howard Hughes Medical Institute
1998
Johns Hopkins Medicine
1998
Freie Universität Berlin
1990-1996
The recognition of odorants by olfactory receptors represents the first stage in odor discrimination. Here, we report generation an expression library containing a large and diverse repertoire mouse receptor sequences transmembrane II–VII region. From this library, 80 chimeric were tested against 26 after transfection into HEK-293 cells. Three identified to respond micromolecular concentrations carvone, (−) citronellal, limonene, respectively. We also found that I7 receptor, unlike rat...
Morphological, electrophysiological, and biochemical properties of H9c2 cells, a permanent cell line derived from rat cardiac tissue, were studied. Although the lectin binding pattern revealed similar sugar residues in surface coat cells isolated cardiocytes, heart-specific morphological structures could not be detected cells. Under physiological ionic conditions, exhibited an outwardly rectifying, transient K+ current. When this current component was blocked by Ba2+ Cs+, we observed inward...
The prohibitin (PHB)-domain proteins are membrane that regulate a variety of biological activities, including mechanosensation, osmotic homeostasis, and cell signaling, although the mechanism this regulation is unknown. We have studied two members large protein family, MEC-2, which needed for touch sensitivity in Caenorhabditis elegans, Podocin, involved function filtration barrier mammalian kidney, find both bind cholesterol. This binding requires PHB domain (including palmitoylation sites...
Abstract Our cellular immune system has to cope constantly with foodborne substances that enter the bloodstream postprandially. Here, they may activate leukocytes via specific but yet mostly unknown receptors. Ectopic RNA expression out of gene families chemosensory receptors, i.e., ∼400 ORs, ∼25 TAS2R bitter-taste and TAS1R umami- sweet-taste receptor dimers by which we typically detect substances, been reported in a variety peripheral tissues unrelated olfaction or taste. In present study,...
Abstract TAAR1 and TAAR2 are necessary for biogenic amine-induced in vitro activation of PMN migration, Th-cell cytokine secretion, B-cell immunoglobulin E production. Certain amines, such as 2-PEA, TYR, or T1AM, modulate blood pressure, cardiac function, brain monoaminergic systems, olfaction-guided behavior by specifically interacting with members a group rhodopsin-like receptors, TAAR. A receptor that is absent from olfactory epithelia but had long been identified the variety peripheral...
FSGS is a CKD with heavy proteinuria that eventually progresses to ESRD. Hereditary forms of have been linked mutations in the transient receptor potential cation channel, subfamily C, member 6 ( TRPC6 ) gene encoding nonselective channel. Most these cause gain-of-function phenotype, leading calcium–triggered podocyte cell death, but underlying molecular mechanisms are unclear. We studied effect disease-related using tridimensional silico modeling tetrameric TRPC6. Our results indicated G757...
Olfactory receptors are the largest group of orphan G protein-coupled with an infinitely small number agonists identified out thousands odorants. The de-orphaning olfactory receptor (OR) is complicated by its combinatorial odorant coding and thus requires large scale screening establishing receptor-specific profiles. Here, we report on stable reconstitution OR-specific signaling in HeLa/Olf cells via protein alphaolf adenylyl cyclase type-III to Ca2+ influx-mediating cyclic nucleotide-gated...
Fenamates are N-phenyl-substituted anthranilic acid derivatives clinically used as non-steroid anti-inflammatory drugs in pain treatment. Reports describing fenamates tools to interfere with cellular volume regulation attracted our attention based on interest the role of volume-modulated transient receptor potential (TRP) channels TRPM3 and TRPV4.Firstly, we measured blocking potencies selectivities TRPV4 well TRPC6 TRPM2 by Ca(2+) imaging heterologous HEK293 cell system. Secondly, further...
Key food odorants are the most relevant determinants by which we detect, recognize, and hedonically evaluate aroma of foods beverages. Odorants detected our chemical sense olfaction, comprising a set approximately 400 different odorant receptor types. However, specific activity patterns representing percepts or beverages, as well key agonist profiles single-odorant receptors, largely unknown. We aimed to establish comprehensive 2 unrelated, broadly tuned OR1A1 OR2W1, that had been associated...
The detection of key food odorants appears to be an important capability odorant receptors. Here, thiols occupy outstanding position among the 230 known because their very low odor thresholds. Members homologous series 3-mercapto-2-methylalkan-1-ols have been described as onion or constituents and are detected at logarithmically different 3-Mercapto-2-methylpentan-1-ol being only within this also has lowest threshold. Most typically activate combinations receptors, which may narrowly broadly...
Furanones formed during the Maillard reaction often are natural aroma-determining compounds found in numerous foods. Prominent economically relevant representatives structural homologues Furaneol and sotolone, which important flavoring because of their distinct caramel- seasoning-like odor qualities. These, however, cannot be predicted by odorants' molecular shape, rather receptors' activation parameters help to decipher encoding quality. Here, qualities sotolone suggested an at least two...
With approximately 400 encoding genes in humans, odorant receptors (ORs) are the largest subfamily of class A G protein-coupled (GPCRs). Despite its high relevance and representation, odorant-GPCRome is structurally poorly characterized: no experimental structures available, low sequence identity ORs to experimentally solved GPCRs a significant challenge for their modeling. Moreover, receptive range most unknown. The receptor OR5K1 was recently comprehensively characterized terms cognate...
With ∼400 olfactory G protein-coupled receptors (GPCR), humans sensitively perceive ∼230 key aroma compounds as best natural agonists of ∼10000 food volatiles. An understanding odorant coding, thus, critically depends on the knowledge about interactions chemicals and their mixtures with cognate receptors. Genetically designed test cell systems enable screening, deorphaning, characterization single (OR). This study shows for aroma-specific quantitative butter recombinate, its components,...
Sulfur-containing compounds within a physiological relevant, natural odor space, such as the key food odorants, typically constitute group of volatiles with lowest thresholds. The observation that certain metals, copper, potentiate smell sulfur-containing, metal-coordinating odorants led to hypothesis their cognate receptors are metalloproteins. However, experimental evidence is sparse—so far, only one human odorant receptor, OR2T11, and few mouse receptors, have been reported be activated...
Mammals perceive a multitude of odorants by their chemical sense olfaction, high-dimensional stimulus-detection system, with hundreds narrowly or broadly tuned receptors, enabling pattern recognition the brain. Cognate receptor-agonist information, however, is sparse, and role odorant receptors for encoding odor quality remains elusive. Here, we screened IL-6-HaloTag®-OR2W1 haplotypes against 187 out 230 defined key food using GloSensor™ system in HEK-293 cells, yielding 48 new agonists....
Clarifying the function of sensory active TRP (transient receptor potential) channels in non-sensory tissue is growing interest, especially with regard to food ingredients nutritionally relevant concentrations. The study hypothesizes TRPV1 agonist [6]-gingerol facilitate cellular immune responses primary human neutrophils, after treatment 50 nM, a concentration that can be reached circulation habitual dietary intake.qRT-PCR analyses reveal high abundancy channel RNA expression types...
A pharmacological classification of receptor-activated nonselective cation channels has not been possible because the lack specific and potent blockers. In dibutyryl-cAMP-differentiated HL-60 cells, we recently identified ATP- N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP)-stimulated currents that were blocked by an organic inhibitor receptor-mediated Ca2+ entry, 1-beta-[3-(4-methoxyphenyl)-propoxy]-4-methoxyphenethyl-1H-imidazole hydrochloride (SK&F 96365), with IC50 about 3 microM....
The assignment of cognate odorant/agonist pairs is a prerequisite for an understanding odorant coding at the receptor level. However, identification new ligands receptors (ORs) in cell-based assays has been challenging, due to their individual and rather sub-optimal plasma membrane expression, as compared with other G protein-coupled receptors. Accessory proteins, such chaperone RTP1S, or Ric8b, have improved surface expression least portion ORs. Typically, recombinant ORs carry N-terminal...