A5035668668- Genomics and Phylogenetic Studies
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Protist diversity and phylogeny
- RNA Research and Splicing
- MicroRNA in disease regulation
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Neuroinflammation and Neurodegeneration Mechanisms
- Plant Reproductive Biology
- Plant and Fungal Species Descriptions
- Retinal Development and Disorders
- Antibiotic Resistance in Bacteria
- Neurogenesis and neuroplasticity mechanisms
- Genetic and phenotypic traits in livestock
- Tea Polyphenols and Effects
- Plant Diversity and Evolution
- Renal and related cancers
- RNA modifications and cancer
- Circular RNAs in diseases
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Microbial bioremediation and biosurfactants
- Immune Cell Function and Interaction
Bioscience (China)
2024
SINOPEC Research Institute of Safety Engineering Co., Ltd.
2024
Chinese Academy of Agricultural Sciences
2015-2024
Agricultural Genomics Institute at Shenzhen
2020-2024
Ministry of Agriculture and Rural Affairs
2020-2024
Sinopec (China)
2024
Dali University
2024
Wuhan University
2019-2023
Renmin Hospital of Wuhan University
2019-2023
Merck (Germany)
2021-2022
Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative comparison RNA transcript abundance. Useful data from this depend on fitting to theoretical curves that allow computation mRNA levels. Calculating accurate levels requires important parameters such as reaction efficiency and fractional cycle number at threshold (CT) be used; however, many algorithms currently in use estimate these parameters. Here we describe an...
Abstract Tea is an economically important plant characterized by a large genome, high heterozygosity, and species diversity. In this study, we assemble 3.26-Gb high-quality chromosome-scale genome for the ‘Longjing 43’ cultivar of Camellia sinensis var. . Genomic resequencing 139 tea accessions from around world used to investigate evolution phylogenetic relationships accessions. We find that hybridization has increased heterozygosity wide-ranging gene flow among populations with spread...
Rationale: Ureteral obstruction-induced hydronephrosis is associated with renal fibrosis and progressive chronic kidney disease (CKD). Exosome-mediated cell-cell communication has been suggested to be involved in various diseases, including fibrosis. However, little known regarding how exosomes regulate obstructed kidneys. Methods: We first examined the secretion of UUO (unilateral ureteral obstruction) mouse kidneys TGF-β1-stimulated tubular epithelial cells (NRK-52E). Exosomes from NRK-52E...
Brn3b/Brn-3.2/POU4f2 is a POU domain transcription factor that essential for retinal ganglion cell (RGC) differentiation, axonal outgrowth and survival. Our goal was to establish link between Brn3b the downstream events leading RGC differentiation. We sought determine both number types of genes depend on their expression. RNA probes from wild-type Brn3b-/- E14.5, E16.5 E18.5 mouse retinas were hybridized microarray containing 18,816 retina-expressed cDNAs. At we identified 87 whose...
Across several cancers, IL18 receptor accessory protein (IL18RAP) is abnormally expressed, and this abnormality related to tumor immunity heterogeneous clinical outcomes. In study, based on bioinformatics analysis, we discovered that IL18RAP the human microenvironment promotes various immune cells infiltration. Additionally, multiple immunofluorescence staining revealed with increased expression of IL18RAP, number infiltrated M1 macrophages increased. This finding was confirmed by coculture...
Whole-genome genotyping (WGG) stands as a pivotal element in genomic-assisted plant breeding. Nevertheless, sequencing-based approaches for WGG continue to be costly, primarily owing the high expenses associated with library preparation and laborious protocol. During prior development of foreground background integrated by sequencing (FBI-seq), we discovered that any sequence-specific primer (SP) inherently possesses capability amplify massive array stable reproducible non-specific PCR...
Abstract Tea is an economically important plant characterized by a large genome size and high heterozygosity species diversity. In this study, we assembled 3.26 Gb high-quality chromosome-scale for tea using the ‘Longjing 43’ cultivar of Camellia sinensis var. . Population resequencing 139 accessions from around world was used to investigate evolution reveal phylogenetic relationships among accessions. With spread cultivation, hybridization has increased wide-ranging gene flow populations....
Renal ischaemia/reperfusion (I/R) injury may induce kidney damage and dysfunction, in which oxidative stress apoptosis play important roles. Long noncoding RNAs (lncRNAs) microRNAs (miRNAs) are reported to be closely related renal I/R, but the specific molecular mechanism is still unclear. The purpose of this research was explore regulatory effect lncRNA TUG1 on I/R injury. This revealed that hypoxia/reperfusion (H/R) vitro, expression level upregulated, levels were negatively correlated...
Hollyhock (Alcea rosea (Linn). Cavan) is an herbaceous flowering plant with significant applications in urban greening, soil remediation, and traditional medicine. However, its genetic diversity molecular characteristics at the population level have not been explored yet. Here, phenotypic of 162 hollyhock accessions from China revealed extensive variation among 11 traits strong correlations between several quantitative traits. Whole-genome re-sequencing 32 randomly chosen identified...
Abstract The leaf angle (LA), plant height (PH), and ear (EH) are key architectural traits influencing maize ( Zea mays L.) yield. However, their genetic determinants have not yet been well‐characterized. Here, we developed a advanced backcross‐nested association mapping population in Henan Agricultural University (HNAU‐NAM1) comprised of 1,625 BC 1 F 4 /BC 2 lines. These were obtained by crossing diverse set 12 representative inbred lines with the common GEMS41 line, which then genotyped...