A5102930256- Obstructive Sleep Apnea Research
- Neuroscience of respiration and sleep
- Cancer, Hypoxia, and Metabolism
- Periodontal Regeneration and Treatments
- Osteoarthritis Treatment and Mechanisms
- Cardiovascular and Diving-Related Complications
- Sleep and Wakefulness Research
- High Altitude and Hypoxia
- Mesenchymal stem cell research
- Adipose Tissue and Metabolism
- Respiratory Support and Mechanisms
- Gut microbiota and health
- Shoulder Injury and Treatment
- Platelet Disorders and Treatments
- Neonatal Respiratory Health Research
- Extracellular vesicles in disease
- Oral and gingival health research
- Tissue Engineering and Regenerative Medicine
- Allergic Rhinitis and Sensitization
- Cardiovascular Function and Risk Factors
- Cardiovascular Disease and Adiposity
- MicroRNA in disease regulation
- Immune cells in cancer
- Immune Cell Function and Interaction
- Chronic Obstructive Pulmonary Disease (COPD) Research
Universidad Cardenal Herrera CEU
2019-2024
Agència de Qualitat i Avaluació Sanitàries de Catalunya
2021
Centro de Investigación Biomédica en Red
2021
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2010-2019
Universitat de Barcelona
2013-2019
Hospital Clínic de Barcelona
2009-2019
Centre for Biomedical Network Research on Rare Diseases
2017-2019
Zero to Three
2016
Oregon State University
2011-2015
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2009-2014
We assessed whether intermittent hypoxia, which emulates one of the hallmarks obstructive sleep apnoea (OSA), leads to altered faecal microbiome in a murine model. In vivo partial pressure oxygen was measured colonic faeces during hypoxia four anesthetised mice. 10 mice were subjected pattern chronic (20 s at 5% O 2 and 40 room air for 6 h·day −1 ) weeks served as normoxic controls. Faecal samples obtained composition determined by 16S rRNA pyrosequencing bioinformatic analysis Quantitative...
Evidence from patients and animal models suggests that obstructive sleep apnea (OSA) may increase the risk of Alzheimer's disease (AD) AD is associated with reduced brain tissue stiffness.To investigate whether intermittent hypoxia (IH) alters cortex stiffness in mutant mice exposed to IH mimicking OSA.Six-eight month old (B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J) wild-type (WT) littermates were subjected (21% O2 40 s 5% 20 s; 6 h/day) or normoxia for 8 weeks. After euthanasia, (E) 200-μm slices was...
Obstructive sleep apnoea (OSA) is associated with higher cancer incidence, tumour aggressiveness and mortality, as well greater severity of infections, which have been attributed to an immune deregulation. We studied the expression programmed cell death (PD)-1 receptor its ligand (PD-L1) on cells from patients OSA, consequences immune-suppressing activity. report that PD-L1 was overexpressed monocytes PD-1 CD8 + T-cells in a severity-dependent manner. overexpression were induced both human...
Intermittent hypoxia (IH) mimicking obstructive sleep apnea (OSA) significantly modifies gut microbiota in mice. However, whether these IH-induced microbiome changes are reversible after restoring normal oxygenation (the equivalent of effective OSA therapy) is unknown. The aim this study was to investigate composition and circulating endotoxemia a post-IH normoxic period mouse model OSA. Ten mice were subjected IH (40 sec 21% O2-20 5% O2) for 6 h/day w 10 breathing air (NM) used as controls....
To test the hypotheses that dynamic changes in brain oxygen partial pressure (PtO(2)) response to obstructive apneas or intermittent hypoxia differ from those other organs and PtO(2) is a source of oxidative stress.Prospective controlled animal study.University laboratory.98 Sprague-Dawley rats.Cerebral cortex, skeletal muscle, visceral fat tissues were exposed anesthetized animals subjected either (apneic hypoxic events 15 s each 60 events/h) for 1 h.Arterial saturation (SpO(2)) presented...
Abstract Background Mesenchymal stromal/stem cells (MSCs) and MSC-derived extracellular vesicles (MSC-EVs) hold promise as a disease modifying treatment in osteoarthritis (OA). Obesity, its associated inflammation, contribute to OA development metabolic represents specific significant group of the patient population. Given their immunomodulatory properties, MSC MSC-EVs are especially interesting for this patients therapeutic option. Here, we were first compare efficacy MSCs mild model taking...
Obstructive sleep apnea (OSA) is characterized by intermittent hypoxia and oxidative stress. However, it unknown whether mimicking OSA modifies male fertility. We tested the hypothesis that fertility reduced chronic in a mouse model. Case-control comparison murine University research laboratory. Eighteen F1 (C57BL/6xCBA) mice. Mice were subjected to pattern of periodic (20 sec at 5% O2 followed 40 room air) 6 h/day for 60 days or normoxia. After this period, mice performed mating trial...
OSA increases atrial fibrillation (AF) risk and is associated with poor AF treatment outcomes. However, a causal association not firmly established the mechanisms involved are poorly understood. The aims of this work were to determine whether chronic obstructive sleep apnea (OSA) induces an pro-arrhythmogenic substrate explore mesenchymal stem cells (MSC) able prevent it in rat model OSA. A custom-made setup was used mimic recurrent OSA-like airway obstructions rats. OSA-rats (n = 16)...
Abstract An adverse role for obstructive sleep apnea (OSA) in cancer epidemiology and outcomes has recently emerged from clinical animal studies. In animals, intermittent hypoxia (IH) mimicking OSA promotes tumor malignancy both directly via host immune alterations. We hypothesized that IH could potentiate aggressiveness through activation of the cyclooxygenase-2 (COX-2) pathway concomitant increases prostaglandin E2 (PGE 2 ). The contribution COX-2 IH-induced enhanced was assessed using...
ABSTRACT Background and objective In obstructive sleep apnoea (OSA), intermittent hypoxia (IH) compromises immune surveillance through the upregulation of programmed cell death‐1 (PD‐1) receptor its ligand (PD‐L1). Because risk OSA‐related cancer depends on age, we assessed PD‐L1/PD‐1 expression in middle‐aged older patients with OSA as well a murine model. Methods PD‐L1 was studied 41 severe 40 healthy volunteers (HV), divided into two groups (≤55 >55 years age). We used flow cytometry,...
BACKGROUND: Tissue hypoxia-reoxygenation characterizes obstructive sleep apnea (OSA), a very prevalent respiratory disease associated with increased cardiovascular morbidity and mortality. Experimental studies indicate that intermittent hypoxia (IH) mimicking OSA induces oxidative stress inflammation in heart tissue at the cell molecular levels. However, it remains unclear whether IH modifies passive stiffness of cardiac extracellular matrix (ECM). AIM: To investigate multiscale changes...
Cognitive impairment is one of the main consequences obstructive sleep apnea (OSA) and usually attributed in part to oxidative stress caused by intermittent hypoxia cerebral tissues. The presence oxygen-reactive species brain tissue should be produced deoxygenation-reoxygenation cycles which occur at level during recurrent apneic events. However, how changes arterial blood oxygen saturation (SpO2) repetitive apneas translate into partial pressure (PtO2) has not been studied. objective this...
To test the hypotheses that brain oxygen partial pressure (PtO2) in response to obstructive apneas changes with age and it might lead different levels of cerebral tissue oxidative stress. Prospective controlled animal study. University laboratory. Sixty-four male Wistar rats: 32 young (3 mo old) aged (18 mo). Protocol 1: Twenty-four animals were subjected (50 apneas/h, lasting 15 sec each) or sham procedure for 50 min. 2: Forty rats 4 h. Real-time PtO2 measurements performed using a...
We investigate the effects of intermittent hypoxia (IH), a characteristic feature obstructive sleep apnea (OSA), on renal cancer progression in an animal and cell model. An vivo mouse model (Balb/c, n = 50) kidney was used to assess effect IH tumor growth, metastatic capacity, angiogenesis immune response. vitro tested RENCA cells, macrophages endothelial cells. Tumor circulating vascular growth factor (VEGF) content associated their phenotype were assessed tumor. In vitro, VEGF expression...
Chronic intermittent hypoxia (CIH) is a major determinant of the cardiovascular morbidity associated with obstructive sleep apnoea (OSA), and magnitude CIH impact may be influenced by ageing. Here, we assessed role ageing in early structural remodelling induced severe murine model OSA.Cardiovascular was young (2 months old, n = 20) aged (18 C57BL/6 female mice exposed to (20% O2 for 40 s, 5% 20 s) or normoxia (room air) 8 weeks (6 h/day).Early vascular observed as illustrated intima-media...
Abstract Study Objectives Chronic intermittent hypoxia (CIH) is a major determinant in obstructive sleep apnea cardiovascular morbidity and this effect influenced by age. The objective of the present study was to assess differential molecular mechanisms at gene-level expression involved remodeling induced CIH according chronological Methods Two- 18-month-old mice (N = 8 each) were subjected or normoxia for weeks. Total messenger RNA (mRNA) extracted from left ventricle myocardium aortic...