A5065661947- Gut microbiota and health
- Brucella: diagnosis, epidemiology, treatment
- Diet and metabolism studies
- Burkholderia infections and melioidosis
- Salmonella and Campylobacter epidemiology
- Dietary Effects on Health
- Adipose Tissue and Metabolism
- Lipid metabolism and disorders
- Nutritional Studies and Diet
- Epigenetics and DNA Methylation
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Obstructive Sleep Apnea Research
- Tea Polyphenols and Effects
- Diet, Metabolism, and Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Phytochemicals and Antioxidant Activities
- Galectins and Cancer Biology
- Bariatric Surgery and Outcomes
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Peroxisome Proliferator-Activated Receptors
- Mycobacterium research and diagnosis
- Circadian rhythm and melatonin
- Bacteriophages and microbial interactions
- Fatty Acid Research and Health
Hospital Clínico Universitario Virgen de la Victoria
2012-2025
Instituto de Investigación Biomédica de Málaga
2015-2024
Universidad de Málaga
2010-2024
Biomedical Research Institute
2024
Roche (Spain)
2022
Centro de Investigación Biomédica en Red de Cáncer
2021
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2013-2020
Instituto de Salud Carlos III
2009-2020
Centro de Investigación Biomédica en Red
2009-2019
Escuela Nacional de Sanidad
2016
A recent study using a rat model found significant differences at the time of diabetes onset in bacterial communities responsible for type 1 modulation. We hypothesized that humans could also be linked to specific gut microbiota. Our aim was quantify and evaluate difference composition microbiota between children with healthy determine possible relationship glycemic level.A case-control carried out 16 children. The fecal bacteria investigated by polymerase chain reaction-denaturing gradient...
Several evidences indicate that gut microbiota is involved in the control of host energy metabolism.To evaluate differences composition rat models under different nutritional status and physical activity to identify their associations with serum leptin ghrelin levels.In a case study, forty male rats were randomly assigned one these four experimental groups: ABA group food restriction free access exercise; no exercise feed ad libitum without libitum. The fecal bacteria was investigated by...
Gut microbiota-related metabolites are potential clinical biomarkers for cardiovascular disease (CVD). Circulating succinate, a metabolite produced by both microbiota and the host, is increased in hypertension, ischemic heart disease, type 2 diabetes. We aimed to analyze systemic levels of succinate obesity, major risk factor CVD, its relationship with gut microbiome. explored association circulating specific metagenomic signatures cross-sectional prospective cohorts Caucasian Spanish...
This study evaluated the possible prebiotic effect of a moderate intake red wine polyphenols on modulation gut microbiota composition and improvement in risk factors for metabolic syndrome obese patients. Ten patients ten healthy subjects were included randomized, crossover, controlled intervention study. After washout period, consumed de-alcoholized over 30 day period each. The dominant bacterial did not differ significantly between groups after two periods. In patients, increased number...
We assessed whether intermittent hypoxia, which emulates one of the hallmarks obstructive sleep apnoea (OSA), leads to altered faecal microbiome in a murine model. In vivo partial pressure oxygen was measured colonic faeces during hypoxia four anesthetised mice. 10 mice were subjected pattern chronic (20 s at 5% O 2 and 40 room air for 6 h·day −1 ) weeks served as normoxic controls. Faecal samples obtained composition determined by 16S rRNA pyrosequencing bioinformatic analysis Quantitative...
OBJECTIVE Type 1 diabetes is associated with compositional differences in gut microbiota. To date, no microbiome studies have been performed maturity-onset of the young 2 (MODY2), a monogenic cause diabetes. Gut microbiota type diabetes, MODY2, and healthy control subjects was compared. RESEARCH DESIGN AND METHODS This case-control study 15 children 13 children. Metabolic potential factors modifying were controlled. Microbiome composition determined by 16S rRNA pyrosequencing. RESULTS...
Real-time PCR is a widely used tool for the diagnosis of many infectious diseases. However, little information exists about influences different factors involved in on amplification efficiency. The aim this study was to analyze effect boiling as DNA preparation method efficiency process real-time human brucellosis with serum samples. Serum samples from 10 patients were analyzed by SYBR green I LightCycler-based and using obtain DNA. prepared lysis bacteria isolated did not prevent presence...
A single-step PCR assay with genus-specific primers for the amplification of a 223-bp region sequence encoding 31-kDa immunogenetic Brucella abortus protein (BCSP31) was used rapid diagnosis human brucellosis. We examined peripheral blood from 47 patients, total 50 cases brucellosis, and group 60 control subjects, composed patients febrile syndromes several etiologies other than asymptomatic subjects seropositive antibodies, healthy subjects. Diagnosis brucellosis established in 35 (70%) by...
The health benefits associated with the consumption of polyphenol-rich foods have been studied in depth, however, full mechanism action remains unknown.
The low-grade inflammation observed in obesity has been associated with a high-fat diet, though this relation is not fully understood. Bacterial endotoxin, produced by gut microbiota, may be the linking factor. However, confirmed obese patients. To study relationship between diet and bacterial we analyzed postprandial endotoxemia morbidly patients after fat overload. endotoxin levels were determined serum chylomicron fraction at baseline 3 h overload 40 their related degree of insulin...
Gut microbiota composition has been reported as a factor linking host metabolism with the development of cardiovascular diseases (CVD) and intestinal immunity. Such gut shown to aggravate CVD by contributing production trimethylamine-N-oxide (TMAO), which is pro-atherogenic compound. Treg cells expressing transcription Forkhead box protein P3 (FoxP3) play an essential role in regulation immune responses commensal have atheroprotective role. However, aim this study was analyze on...
Alterations of gut microbiome have been proposed to play a role in metabolic disease, but the major determinants microbiota composition remain ill defined. Nutritional and sex hormone challenges, especially during early development, shown permanently alter adult female phenotype contribute disturbances. In this study, we implemented large-scale analyses fecal samples from groups rats sequentially subjected various obesogenic manipulations, including perturbations by means neonatal...
Intermittent hypoxia (IH) mimicking obstructive sleep apnea (OSA) significantly modifies gut microbiota in mice. However, whether these IH-induced microbiome changes are reversible after restoring normal oxygenation (the equivalent of effective OSA therapy) is unknown. The aim this study was to investigate composition and circulating endotoxemia a post-IH normoxic period mouse model OSA. Ten mice were subjected IH (40 sec 21% O2-20 5% O2) for 6 h/day w 10 breathing air (NM) used as controls....
Obesity is considered an important factor that increases the risk of colorectal cancer (CRC). So far, association gut microbiota with both obesity and has been described independently. Nevertheless, a specific obesity-related microbial profile linked to CRC development not identified. The aim this study was determine composition in fecal samples from patients (OB-CRC) without (L-CRC) compared present non-obese healthy controls (L-HC), order unravel possible relationship between...
The exposome, defined as the cumulative measure of external exposures and associated biological responses throughout lifespan, has emerged in recent years a cornerstone biomedical sciences. Metabolomics stands out here one most powerful tools for investigating interplay between genetic background, exogenous, endogenous factors within human health. However, to address complexity novel methods are needed characterize metabolome. In this work, we have optimized validated multianalyte...
Abstract Context Metabolic dysfunction–associated steatotic liver disease (MASLD) is characterized by the intracellular lipid accumulation in hepatocytes. Excess caloric intake and high-fat diets are considered to significantly contribute MASLD development. Objective To evaluate hepatic serum fatty acid (FA) composition patients with different stages of MASLD, their relationship FA dietary MASLD-related risk factors. Methods This was a case–control study obesity undergoing bariatric surgery...
ABSTRACT We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for diagnosis human brucellosis. found that high concentrations leukocyte and heme compounds inhibit PCR. These inhibitors be efficiently suppressed by increasing number washings to four or five decreasing amount total 2 4 μg, thereby avoiding false-negative results.