A5055428607- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neural Engineering
- Advanced Sensor and Energy Harvesting Materials
- Silk-based biomaterials and applications
- Bone Tissue Engineering Materials
- Osteoarthritis Treatment and Mechanisms
- Conducting polymers and applications
- Cellular Mechanics and Interactions
- Angiogenesis and VEGF in Cancer
- Axon Guidance and Neuronal Signaling
- Advanced Materials and Mechanics
- Pluripotent Stem Cells Research
- Graphene and Nanomaterials Applications
- Phosphodiesterase function and regulation
- Supercapacitor Materials and Fabrication
- Pancreatic function and diabetes
- Supramolecular Self-Assembly in Materials
- Wound Healing and Treatments
- Additive Manufacturing and 3D Printing Technologies
- Hydrogels: synthesis, properties, applications
- Polymer composites and self-healing
- Cell Image Analysis Techniques
Maastricht University
2016-2025
Inspire
2025
National MS Center Melsbroek
2023
Hasselt University
2023
University of Twente
2009-2020
Italian Institute of Technology
2012-2020
Scuola Superiore Sant'Anna
2010-2020
Piaggio Aerospace (Italy)
2012-2020
Center for Micro-BioRobotics
2012-2020
Piaggio (Italy)
2012-2013
Nanofibrous structures have long been used as scaffolds for tissue engineering (TE) applications, due to their favorable characteristics, such high porosity, flexibility, cell attachment and enhanced proliferation, overall resemblance native extracellular matrix (ECM). Such can be easily produced at a low cost via electrospinning (ESP), but generally cannot fabricated with regular and/or complex geometry, characterized by macropores uniform thickness. We present here novel technique direct...
The creation of skeletal muscle tissue in vitro is a major topic interest today the field biomedical research, due to lack treatments for loss traumatic accidents or disease. For this reason, intrinsic properties nanofibrillar structures promote cell adhesion, proliferation, and alignment presents an attractive tool regenerative medicine recreate organized tissues such as muscle. Electrospinning one processing techniques often used fabrication these nanofibrous combination synthetic natural...
Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS) characterized by focal inflammatory lesions and prominent demyelination. Even though currently available therapies are effective in treating initial stages disease, they unable to halt or reverse progression into progressive stage. Thus far, no repair-inducing treatments for MS patients. Hence, there an urgent need development new therapeutic strategies either targeting destructive immunological...
Cartilage tissue presents low self-repair capability and lesions often undergo irreversible progression. Structures obtained by engineering, such as those based in extrusion bioprinting of constructs loaded with stem cell spheroids may offer valuable alternatives for research therapeutic purposes. Human mesenchymal stromal (hMSC) can be chondrogenically differentiated faster more efficiently than single cells. This approach allows obtaining larger tissues a rapid, controlled reproducible...
Peripheral nerve damage results in the loss of sensorimotor and autonomic functions, which is a significant burden to patients. Furthermore, injuries greater than limiting gap length require surgical repair. Although autografts are preferred clinical choice, their usage impeded by limited availability, dimensional mismatch, sacrifice another functional donor nerve. Accordingly, guidance conduits, tubular scaffolds engineered provide biomimetic environment for regeneration, have emerged as...
Development of new nerve guides is required for replacing autologous grafts the repair long gap defects after injury. A guide comprised only electrospun fibers able to bridge a critical (15 mm) in rat animal model reported first time. The conduits are made poly(ethylene oxide terephthalate) and poly(butylene (PEOT/PBT), biocompatible copolymer composed alternating amorphous, hydrophilic terephthalate), crystalline, hydrophobic segments. These show suitable mechanical properties, high...
Smart polymeric biomaterials have been the focus of many recent biomedical studies, especially those with adaptability to defects and potential be implanted in human body. Herein we report a versatile straightforward method convert non-thermoresponsive hydrogels into thermoresponsive systems shape memory ability. As proof concept, polyurethane mesh was embedded within methacrylated chitosan (CHTMA), gelatin (GELMA), laminarin (LAMMA) or hyaluronic acid (HAMA) hydrogel network, which afforded...
The fabrication process of ceramic yttria-stabilized zirconia (YSZ) and nickel oxide nanofibers by electrospinning is reported. preparation hollow YSZ aligned nanofiber arrays also demonstrated. influence the parameters process, physicochemical properties spinning solutions, thermal treatment procedure on spinnability final microstructure fibers was determined. fiber diameter can be varied from hundreds nanometers to more than a micrometer controlling solution while grain size surface...
Supramolecular and dynamic biomaterials hold promise to recapitulate the time-dependent properties stimuli-responsiveness of native extracellular matrix (ECM). Host–guest chemistry is one most widely studied supramolecular bonds, yet binding characteristics host–guest complexes (β-CD/adamantane) in relevant have mostly focused on singular interactions or nondiscrete multivalent pendent polymers. The stepwise synergistic effect for formation remains relatively unreported. In this work, we...
Changes in actin structure and distribution are involved many cellular processes, such as differentiation, proliferation migration. Differences cell shape size make the analysis of difficult. Here, we have developed a Fiji macro that analyzes within cell, regardless or shape. The staining intensity is measured along an automatically drawn line over cell. data divided equal bins, making insensitive to changes We also created R script further processes acquired data. Together, final can be...
The field of human induced pluripotent stem cells (hiPSCs) has seen significant progress since the discovery reprogramming somatic using transcription factors Oct4, Sox2, Klf4, and c-Myc. hiPSCs are similar to embryonic in a primed state pluripotency potential differentiate into any adult cell type, offering versatile tool for research therapeutic applications. However, efficiency differentiation protocols generating complex structures with multiple types, like kidney organoids, remains...
ABSTRACT Cell culture of neuronal cells places high demands on the surface for these to adhere and grow on. Native extracellular matrix (ECM) proteins are often applied cell surface. The substrate is even more important when mechanical strain in culture. These will easily detach die, precluding study how factors affect cells. Mechanical are, example, eye disorder glaucoma, which characterized by loss retinal ganglion (RGCs), neurons that transfer visual information from retina via optic...
The F11 hybridoma, a dorsal root ganglion-derived cell line, was used to investigate the response of nociceptive sensory neurons nanotopographical guidance cues. This established this line as model peripheral neuron growth for tissue scaffold design. Cells were seeded on substrates cyclic olefin copolymer (COC) films imprinted via nanoimprint lithography (NIL) with grating pattern nano-scale grooves and ridges. Different ridge widths employed alter focal adhesion formation, thereby changing...
Abstract The extracellular matrix (ECM) is a three-dimensional (3D) structure composed of proteinaceous fibres that provide physical and biological cues to direct cell behaviour. Here, we build library hybrid collagen-polymer fibrous scaffolds with nanoscale dimensions screen them for their ability grow chondrocytes cartilage repair. Poly(lactic acid) poly (lactic-co-glycolic at two different monomer ratios (85:15 50:50) were incrementally blended collagen. Physical properties (wettability...
In vitro peripheral nerve models provide valuable tools to study neurobiology questions and assess drug performance, in a regenerative or pathology context. To this end, we have developed representative model of the that displays three-dimensional (3D) neural anisotropy myelination, which showcase here as simple low-cost platform for screening. The is composed three main parts, including rat primary Schwann cells (SCs) seeded onto an electrospun scaffold create bands Büngner (BoB), primed...
The dual role of macrophages in the healing process depends on macrophage ability to polarize into phenotypes that can propagate inflammation or exert anti-inflammatory and tissue-remodeling functions. Controlling scaffold geometry has been proposed as a strategy influence behavior favor positive host response implants. Here, we fabricated Polycaprolactone (PCL) scaffolds by Melt Electrowriting (MEW) investigate architecture modulate polarization. Primary human unpolarized (M0) polarized M1,...