A5056080212- Nerve injury and regeneration
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Nerve Injury and Rehabilitation
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Venomous Animal Envenomation and Studies
- Aquaculture disease management and microbiota
- Bone Tissue Engineering Materials
- Peripheral Nerve Disorders
- Neurogenesis and neuroplasticity mechanisms
- Additive Manufacturing and 3D Printing Technologies
- Glioma Diagnosis and Treatment
- Nanoplatforms for cancer theranostics
Biomedical Research Networking Center on Neurodegenerative Diseases
2022-2024
Universitat Autònoma de Barcelona
2021-2024
Instituto de Salud Carlos III
2022-2023
Centro de Investigación Biomédica en Red
2022
Peripheral nerve damage results in the loss of sensorimotor and autonomic functions, which is a significant burden to patients. Furthermore, injuries greater than limiting gap length require surgical repair. Although autografts are preferred clinical choice, their usage impeded by limited availability, dimensional mismatch, sacrifice another functional donor nerve. Accordingly, guidance conduits, tubular scaffolds engineered provide biomimetic environment for regeneration, have emerged as...
Despite advances in microsurgery, full functional recovery of severe peripheral nerve injuries is not commonly attained. The sheep appears as a good preclinical model since it presents nerves with similar characteristics to humans. In this study, we induced 5 or 7 cm resection the peroneal and repaired an autograft. Functional evaluation was performed monthly. Electromyographic ultrasound tests were at 6.5 9 months postoperation (mpo). No significant differences found between groups respect...
Decellularized nerve allografts are an alternative to autograft for repairing severe injuries, since they have higher availability and do not induce rejection. In this study, we assessed the regenerative potential of a novel decellularization protocol human rat nerves resections, compared gold standard autograft. A 15-mm gap in sciatic was repaired with decellularized allograft (DC-RA), xenograft (DC-HX), or fresh (AG). Electrophysiology tests were performed monthly evaluate muscle...
Decellularized nerve allografts (DC) are an alternative to autografts (AG) for repairing severe peripheral injuries. We have assessed a new DC provided by VERIGRAFT. The decellularization procedure completely removed cellularity while preserving the extracellular matrix. first in 15 mm gap sciatic of rats, showing slightly delayed but effective regeneration. Then, we assayed 70 peroneal sheep compared with AG. Evaluation regeneration and functional recovery was performed clinical,...
Abstract Engineered grafts constitute an alternative to autologous transplant for repairing severe peripheral nerve injuries. However, current clinically available solutions have substantial limitations and are not suited the repair of long defects. A novel design conduit is presented here, which consists a chitosan porous matrix embedding 3D‐printed poly‐ε‐caprolactone mesh. These materials selected due their high biocompatibility, safe degradability, ability support regeneration process....
BACKGROUND AND OBJECTIVES: Peripheral nerve injuries resulting in a defect require surgical repair. The gold standard of autograft (AG) has several limitations, and therefore, new alternatives must be developed. main objective this study was to assess regeneration through long gap injury (50 mm) the peroneal sheep with decellularized allograft (DCA). METHODS: A 5-cm made repaired using an AG or DCA. Functional tests were performed once month electrophysiology echography evaluations at 6.5 9...
Antimicrobial peptides (AMPs) are regarded as a promising alternative to traditional antibiotics in the face of ever-increasing resistance. Our research has focused on crotalicidin (Ctn), an AMP from snake venom, and fragment thereof, Ctn[15-34], with improved vitro antimicrobial anticancer activities remarkable serum stability. As retroenantio versions both AMPs maintained favorable profiles, this work we evaluate vivo efficacy na-tive-sequence their counterparts murine infection model...
Antimicrobial peptides (AMPs) are regarded as a promising alternative to traditional antibiotics in the face of ever-increasing resistance. However, many AMPs fail progress into clinics due unexpected difficulties found preclinical vivo phases. Our research has focused on crotalicidin (Ctn), an AMP from snake venom, and fragment thereof, Ctn[15-34], with improved vitro antimicrobial anticancer activities remarkable serum stability. As retroenantio versions both maintained favorable profiles,...
Abstract Background and Aims Cell culture is a fundamental experimental tool for understanding cell physiology. However, translating these findings to in vivo settings has proven challenging. Replicating donor tissue conditions, including oxygen levels, crucial achieving meaningful results. Nevertheless, conditions are often overlooked, particularly the context of chemotherapy‐induced neurotoxicity. Methods In this study, we investigated role levels primary neuronal cultures by comparing...
Background: Decellularized human nerves overcome the limitations of current treatments for large peripheral nerve injuries. However, use decellularized requires an "off-the-shelf" availability useful and actual clinical application. In this study, we addressed preservation native matrix in integrative approach tissue scaffold production. Materials Methods: For analysis, used histological examination immunofluorescence to examine structure, biomechanical assays evaluate tensile strength...
3D-Printed Nerve Conduits Engineered nerve conduits (NCs) efficacy in restoring limiting gap lesions is still poor, due to low biomimicry. In article 2300136, Silvestro Micera, Xavier Navarro, and co-workers present an NC design that for the first time combines 3D-printing freeze-drying techniques yield with tunable physiochemical properties. NCs restore of rat's sciatic can be therefore considered alternative autologous transplant. Image credit: Dr. Alessio Tommasetti, D'Arc Studio.