A5061113579- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Neuroscience and Neural Engineering
- Microfluidic and Bio-sensing Technologies
- Electrohydrodynamics and Fluid Dynamics
- Advanced X-ray and CT Imaging
- Radiation Effects in Electronics
- Cardiomyopathy and Myosin Studies
- Chemotherapy-induced cardiotoxicity and mitigation
- Cardiac electrophysiology and arrhythmias
- Radiomics and Machine Learning in Medical Imaging
- Cell Image Analysis Techniques
- Artificial Intelligence in Healthcare and Education
University of Twente
2020-2025
Max Planck University of Twente Center for Complex Fluid Dynamics
2022-2025
Chan Heart Rhythm Institute
2022
Sensors (United States)
2022
Cardiovascular disease is often associated with cardiac remodeling, including fibrosis, which may lead to increased stiffness of the heart wall. This in turn cause subsequent failure myocytes, however response these cells substrate largely unknown. To investigate contractile human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) stiffness, we generated a stable transgenic cell line expressing fusion protein α-Actinin and fluorescent mRubyII previously characterized NKX2.5-GFP reporter...
Organoids are engineered 3D miniature tissues that defined by their organ-like structures, which drive a fundamental understanding of human development. However, current organoid generation methods associated with low production throughputs and poor control over size function including due to merging, limits clinical industrial translation. Here, we present microfluidic platform for the mass lumenogenic embryoid bodies functional cardiospheres. Specifically, apply triple-jet in-air...
Advanced in vitro models that recapitulate the structural organization and function of human heart are highly needed for accurate disease modeling, more predictable drug screening, safety pharmacology. Conventional 3D Engineered Heart Tissues (EHTs) lack heterotypic cell complexity culture under flow, whereas microfluidic Heart-on-Chip (HoC) general configuration contractile readouts. In this study, an innovative user-friendly HoC model is developed to overcome these limitations, by...
Engineered heart tissues (EHTs) have shown great potential in recapitulating tissue organization, functions, and cell-cell interactions of the human vitro . Currently, multiple EHT platforms are used by both industry academia for different applications, such as drug discovery, disease modelling, fundamental research. The tissues’ contractile force, one main hallmarks function maturation level cardiomyocytes, can be read out from optically tracking movement elastic pillars induced tissues....
Cardiomyocytes derived from human pluripotent stem cells (hPSC-CMs) hold a great potential as in vitro models for studying heart disease and drug safety screening. Nevertheless, their associated immaturity relative to the adult myocardium limits utility cardiac research. In this study, we describe development of platform generating three-dimensional engineered tissues (EHTs) hPSC-CMs measurement force while under mechanical electrical stimulation. The modular versatile EHT presented here...
Cardiotoxicity remains a major cause of drug withdrawal, partially due to lacking predictability animal models. Additionally, risk cardiotoxicity following treatment cancer patients is limiting. It unclear which will develop heart failure therapy. Human pluripotent stem cell (hPSC)-derived cardiomyocytes present an unlimited source and may offer individualized solutions this problem. We developed platform predict molecular functional aspects cardiotoxicity. Our can discriminate between the...
The use of Engineered Heart Tissues (EHT) as in vitro model for disease modeling and drug screening has increased, they provide important insight into the genetic mechanisms, cardiac toxicity or responses. Consequently, this highlighted need a standardized, unbiased, robust automatic way to analyze hallmark physiological features EHTs. In study we described validated standalone application EHTs an automatic, robust, unbiased way, using low computational time. “ EHT Analysis ” contains two...
Abstract The high rate of drug withdrawal from the market due to cardiovascular toxicity or lack efficacy, economic burden, and extremely long time before a compound reaches market, have increased relevance human in vitro models like (patient‐derived) pluripotent stem cell (hPSC)‐derived engineered heart tissues (EHTs) for evaluation efficacy compounds at early phase development pipeline. Consequently, EHT contractile properties are highly relevant parameters analysis cardiotoxicity, disease...
The synchronization of the electrical and mechanical coupling assures physiological pump function heart, but life-threatening pathologies may jeopardize this equilibrium. Recently, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have emerged as a model for personalized investigation because they can recapitulate diseased traits, such compromised capacity or circuit disruption. This research avails hiPSC-CMs showcases innovative techniques to study properties well their...
Introduction: Cardiotoxicity is one of the main adverse effects cancer therapy and has been extensively assessed in animal models which are not predictive for drug-induced cardiotoxicity humans. Human pluripotent stem cell (hPSC)-derived cardiomyocytes provide a reliable source human have already proven valuable studies. We present here versatile screening platform, utilizing hPSC-cardiomyocytes, assessing different aspects cardiotoxicity. Methods Results: A well-known group anticancer drugs...