A5015735613- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Wound Healing and Treatments
- High Altitude and Hypoxia
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Diabetic Foot Ulcer Assessment and Management
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Cardiovascular and exercise physiology
- Bone Metabolism and Diseases
- Periodontal Regeneration and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Muscle metabolism and nutrition
- Cancer-related molecular mechanisms research
- Tendon Structure and Treatment
- Circular RNAs in diseases
- Bone health and treatments
- MicroRNA in disease regulation
- Shoulder Injury and Treatment
- Additive Manufacturing and 3D Printing Technologies
- Body Contouring and Surgery
- Hip and Femur Fractures
Brigham and Women's Hospital
2018-2025
Harvard University
2018-2025
Creative Commons
2021-2023
Texas Tech University Health Sciences Center
2023
Texas Tech University
2023
Huazhong University of Science and Technology
2022
Tongji Hospital
2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
Christie's
2022
Harvard University Press
2022
Diabetic wound (DW) therapy is currently a big challenge in medicine and strategies to enhance neurogenesis angiogenesis have appeared be promising direction. However, the current treatments failed coordinate simultaneously, leading an increased disability rate caused by DWs. Herein, whole-course-repair system introduced hydrogel concurrently achieve mutually supportive cycle of neurogenesis-angiogenesis under favorable immune-microenvironment. This can first one-step packaged syringe for...
Abstract At present, developing therapeutic strategies to improve wound healing in individuals with diabetes remains challenging. Exosomes represent a promising nanomaterial from which microRNAs (miRNAs) can be isolated. These miRNAs have the potential exert effects, and thus, determining contributions of specific circulating exosomes is great importance. In present study, exosomal are identified diabetic patients assessed for their roles context healing. A significant upregulation...
Acute and chronic wounds affect millions of people around the world, imposing a growing financial burden on patients hospitals. Despite application current wound management strategies, physiological healing process is disrupted in many cases, resulting impaired healing. Therefore, more efficient easy-to-use treatment modalities are needed. In this study, we demonstrate benefit vivo printed, growth factor-eluting adhesive scaffolds for full-thickness porcine model. A custom-made handheld...
Abstract Extremity skeletal muscle injuries result in substantial disability. Current treatments fail to recoup function, but properly designed and implemented tissue engineering regenerative medicine techniques can overcome this challenge. In study, a nanoengineered, growth factor‐eluting bioink that utilizes Laponite nanoclay for the controlled release of vascular endothelial factor (VEGF) GelMA hydrogel supportive adhesive scaffold be crosslinked vivo is presented. The delivered with...
Diabetic wounds remain a great challenge for clinicians due to the multiple bacterial infections and oxidative damage. Exosomes, as an appealing nanodrug delivery system, have been widely applied in treatment of diabetic wounds. Endovascular cells are important component vascular wall. Herein, we investigated effects HUCMSCs HUC-Exos (exosomes secreted by HUCMSCs) on wound healing. In this study, HUVECs were coincubated with HUCMSCs, utilized vitro vivo experiments verify their roles...
Poor cellular spreading, proliferation, and infiltration, due to the dense biomaterial networks, have limited success of most thick hydrogel-based scaffolds for tissue regeneration. Here, inspired by whipped cream production widely used in pastries, foam bioinks are developed bioprinting scaffolds. Upon cross-linking, a multiscale interconnected porous structure, with pores ranging from few several hundreds micrometers, is formed within printed constructs. The effect process parameters on...
Fracture healing is a complex process involving various cell types, cytokines, and mRNAs. Here, we report the roles of circRNA AFF4/miR-7223-5p/PIK3R1 axis during fracture healing. We found that increased expression PIK3R1 directly associated with augmented proliferation decreased apoptosis MC3T3-E1 cells. Furthermore, miR-7223-5p targeted PI3KR1 inhibited while promoting apoptosis. CircRNA AFF4 acted as sponge miR-7223-5p, thereby inhibiting Local injection into femoral sites promoted in...
Emerging evidence highlights the role of long noncoding RNA (lncRNA) KCNQ1OT1 in fracture healing. Osteoblast proliferation, migration, and survival are pivotal during this process. In study, we aimed to improve our understanding regulatory lncRNA osteoblast survival. We searched gene expression omnibus databases LncBase Experimental V.2 identify key microRNAs (miRNAs) targets KCNQ1OT1. MiR-701-3p was selected as a differentially expressed miRNA immunoprecipitation assays were performed...
A major impediment preventing normal wound healing is insufficient vascularization, which causes hypoxia, poor metabolic support, and dysregulated physiological responses to injury. To combat this, the delivery of angiogenic factors, such as vascular endothelial growth factor (VEGF), has been shown provide modest improvement in healing. Here, importance specialty systems explored controlling bed drug distribution consequently improving rate quality. Two intradermal systems, miniaturized...
Abstract Interleukin‐10 (IL‐10) displays well‐documented anti‐inflammatory effects, but its effects on osteoblast differentiation have not been investigated. In this study, we found IL‐10 negatively regulates microRNA‐7025‐5p (miR‐7025‐5p), the down‐regulation of which enhances differentiation. Furthermore, through luciferase reporter assays, evidence that insulin‐like growth factor 1 receptor (IGF1R) is a miR‐7025‐5p target gene positively vivo studies indicated pre‐injection leads to...
Abstract Background Postmenopausal bone loss, mainly caused by excessive resorption mediated osteoclasts, has become a global public health burden. Metformin, hypoglycemic drug, been reported to have beneficial effects on maintaining health. However, the role and underlying mechanism of metformin in ovariectomized (OVX)-induced loss is still vague. Results In this study, we demonstrated for first time that administration alleviated postmenopausal women mice, based reduced markers, increased...
Infection by pathogenic bacteria presents a serious public health issue due to high morbidity and death rates. To aid infective wound closure healing, variety of anti-bacterial biomaterials with optimized for multiple functions including drug delivery, biosensing, or tissue engineering have been designed manufactured. Ti3C2 gold nanoparticle (Au NPs) were chosen as the building blocks self-assembling biomaterial. The materials characterized, then anti-biofilm antibacterial properties...
The roles of long non-coding RNAs (lncRNAs) and micro (miRNAs) as regulators mRNA expression in various diseases have recently been reported. Osteoblast differentiation is the vital process which mediates bone formation fracture healing. In present study, we found microRNA-6979-5p (miR-6979-5p) to be most differentially expressed miRNA between normal calluses mice, overexpression miR-6979-5p was negatively associated with osteoblast differentiation. Through luciferase assays, evidence that...
Abstract Aging is associated with loss of skeletal muscle regeneration. Differentially regulated vascular endothelial growth factor (VEGF)A aging may partially underlies this regenerative capacity. To assess the role VEGFA in regeneration, young (12–14 weeks old) and old C57BL/6 mice (24,25 months are subjected to cryoinjury tibialis anterior (TA) induce The average cross‐sectional area (CSA) regenerating myofibers 33% smaller as compared ( p < 0.01) mice, which correlates a two‐fold...
Bone mesenchymal stem cells (BMSCs) play an important role in maintaining the dynamic balance of bone metabolism. Recent studies have reported that a decrease osteogenic function MSCs is strongly associated with osteoporosis. Melatonin neuroendocrine hormone produced pineal gland and essential physiological regulation. This study aimed at exploring effect melatonin on osteoblastic differentiation elucidate underlying mechanisms. We isolated BMSCs from rat marrow demonstrated improved by...
Abstract Volumetric muscle loss (VML) is a segmental of skeletal which commonly heals with fibrosis, minimal regeneration, and strength. Treatment options for these wounds promote functional recovery are currently lacking. This study was designed to investigate whether the collagen‐GAG scaffold (CGS) promotes following VML. A total 66 C57/Bl6 mice were used in three‐stage experiment. First, 24 animals split into three groups underwent sham injury or unilateral quadriceps VML without CGS...
Background. Machine perfusion (MP) has evolved as a promising approach for the ex situ preservation in organ transplantation. However, literature on use of MP human vascularized composite allografts is scarce. The aim this study was to evaluate effects hypothermic with an acellular perfusate upper extremities and compare current gold standard static cold storage (SCS). Methods. Six were assigned either (n = 3) or SCS conditions 24 h. MP-extremities perfused oxygenated Steen solution at...
Therapeutic strategies that successfully combine two techniques-autologous micrografting and biodegradable scaffolds-offer great potential for improved wound repair decreased scarring. In this study we evaluate the efficacy of a novel modification collagen-glycosaminoglycan (collagen-GAG) scaffold with autologous micrografts using murine dorsal model. db/db mice underwent full thickness 1.0 cm2dorsal excision were treated collagen-GAG (CGS group), modified + MG group) or simple occlusive...