A5061756881- Glioma Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- MRI in cancer diagnosis
- Medical Imaging Techniques and Applications
- Epigenetics and DNA Methylation
- Advanced MRI Techniques and Applications
- Ferroptosis and cancer prognosis
- Single-cell and spatial transcriptomics
- Brain Tumor Detection and Classification
- Neural dynamics and brain function
- Vascular Malformations Diagnosis and Treatment
- Extracellular vesicles in disease
- Advanced Fluorescence Microscopy Techniques
- Advanced Memory and Neural Computing
- Cancer Genomics and Diagnostics
- Neuroscience and Neural Engineering
- Epilepsy research and treatment
- Traumatic Brain Injury and Neurovascular Disturbances
- MicroRNA in disease regulation
- Cardiac Arrest and Resuscitation
- Advanced Radiotherapy Techniques
- Trauma and Emergency Care Studies
- Cancer-related molecular mechanisms research
- Meningioma and schwannoma management
- Photoacoustic and Ultrasonic Imaging
Amsterdam Neuroscience
2019-2024
Amsterdam University Medical Centers
2019-2024
Vrije Universiteit Amsterdam
2019-2024
Cancer Center Amsterdam
2020-2023
University of Amsterdam
2023
Amsterdam UMC Location University of Amsterdam
2023
Addenbrooke's Hospital
2020-2021
University of Cambridge
2020-2021
Amsterdam UMC Location Vrije Universiteit Amsterdam
2012-2019
Jackson Laboratory
2017
Abstract Central nervous system tumours represent one of the most lethal cancer types, particularly among children 1 . Primary treatment includes neurosurgical resection tumour, in which a delicate balance must be struck between maximizing extent and minimizing risk neurological damage comorbidity 2,3 However, surgeons have limited knowledge precise tumour type prior to surgery. Current standard practice relies on preoperative imaging intraoperative histological analysis, but these are not...
Tumor segmentation of glioma on MRI is a technique to monitor, quantify and report disease progression. Manual the gold standard but very labor intensive. At present quality this not known for different stages disease, prior work has mainly focused treatment-naive glioblastoma. In paper we studied inter-rater agreement manual glioblastoma WHO grade II-III novices experts at three disease. We also impact inter-observer variation extent resection growth rate. 20 patients with IV (defined as...
Abstract Background Surgical resection and irradiation of diffuse glioma are guided by standard MRI: T2/fluid attenuated inversion recovery (FLAIR)–weighted MRI for non-enhancing T1-weighted gadolinium-enhanced (T1G) enhancing gliomas. Amino acid PET has been suggested as the new standard. Imaging combinations may improve amino PET. The aim study was to determine accuracy imaging detect infiltration. Methods We included 20 consecutive adults with newly diagnosed (7 astrocytomas, isocitrate...
Human cortical pyramidal neurons are large, have extensive dendritic trees, and yet unexpectedly fast input-output properties: Rapid subthreshold synaptic membrane potential changes reliably encoded in timing of action potentials (APs). Here, we tested whether biophysical properties voltage-gated sodium (Na
Distinguishing tumors from normal brain cells is important but challenging in glioma surgery due to the lack of clear interfaces between two. The ability label-free third harmonic generation (THG) microscopy combination with automated image analysis quantitatively detect infiltration fresh, unprocessed tissue real time assessed. THG images reveal increased cellularity grades II-IV samples 23 patients, as confirmed by subsequent hematoxylin and eosin histology. An quantification workflow...
Intratumoral heterogeneity is a hallmark of diffuse gliomas. DNA methylation profiling an emerging approach in the clinical classification brain tumors. The goal this study to investigate effects intratumoral on confidence.We used neuronavigation acquire 133 image-guided and spatially separated stereotactic biopsy samples from 16 adult patients with glioma (7 IDH-wildtype 2 IDH-mutant glioblastoma, 6 astrocytoma, 1 oligodendroglioma, 1p19q codeleted), which we characterized using arrays....
Glioma imaging, used for diagnostics, treatment planning, and follow-up, is currently based on standard magnetic resonance imaging (MRI) modalities (T1 contrast-enhancement gadolinium-enhancing gliomas T2 fluid-attenuated inversion recovery hyperintensity nonenhancing gliomas). The diagnostic accuracy of these techniques the delineation suboptimal.To assess advanced neuroimaging compared with MRI detection diffuse glioma infiltration within brain.A monocenter, prospective, observational...
This study identified the optimal tracer kinetic model for quantification of dynamic O-(2-[18F]fluoroethyl)-L-tyrosine ([18F]FET) positron emission tomography (PET) studies in seven patients with diffuse glioma (four glioblastoma, three lower grade glioma). The performance more simplified approaches was evaluated by comparison compartment model. Additionally, relationship cerebral blood flow—determined [15O]H2O PET—was investigated. reversible two-tissue Agreement analysis binding potential...
Positron emission tomography (PET) is increasingly used to guide local treatment in glioma. The purpose of this study was a direct comparison two potential tracers for detecting glioma infiltration, O-(2-[18F]-fluoroethyl)-L-tyrosine ([18F] FET) and [11C] choline.Eight consecutive patients with newly diagnosed diffuse underwent dynamic choline [18F] FET PET scans. Preceding craniotomy, multiple stereotactic biopsies were obtained from regions inside outside abnormalities. Biopsies assessed...
Quantitative parametric images of O-(2-[ 18 F]fluoroethyl)-L-tyrosine kinetics in diffuse gliomas could be used to improve glioma grading, tumour delineation or the assessment uptake distribution this positron emission tomography tracer. In study, several and tumour-to-normal maps were compared terms accuracy region averages (when results from nonlinear regression a reversible two-tissue compartment plasma input model) image noise using 90 min dynamic scan data acquired seven patients with...
New technologies developed to improve survival outcomes for glioblastoma (GBM) continue have limited success. Recently, image-guided dose painting (DP) radiotherapy has emerged as a promising strategy increase local control rates. In this study, we evaluate the practical application of multiparametric MRI model glioma infiltration DP in GBM by measuring its conformity, feasibility, and expected clinical benefits against standard care treatment.Maps tumor probability were generated from...
Glioblastoma (GBM) patients have a dismal prognosis. Tumours typically recur within months of surgical resection and post-operative chemoradiation. Multiparametric magnetic resonance imaging (mpMRI) biomarkers promise to improve GBM outcomes by identifying likely regions infiltrative tumour in probability (TP) maps. These could be treated with escalated dose via dose-painting radiotherapy achieve higher rates control. Crucial the technical validation using is repeatability derived...
ABSTRACT Glioma intratumoral heterogeneity enables adaptation to challenging microenvironments and contributes universal therapeutic resistance. Here, we integrated 914 single-cell DNA methylomes, 55,284 transcriptomes, bulk multi-omic profiles across 11 adult IDH-mutant or IDH-wild-type gliomas delineate sources of heterogeneity. We found that local methylation instability, epimutation burden, was elevated in more aggressive tumors, reflected variability, linked with transcriptional...
Abstract Diffuse gliomas are the most prevalent primary malignant brain tumors in adults and characterized by invasive growth poor prognosis. Tumor cells infiltrate parenchyma spread to distant sites. The aim of this study is characterize spatial heterogeneity, mode, direction tumor development identify key areas for localized treatment. Neuronavigation was employed collect n=134 image-guided samples from n=16 adult patients with diffuse gliomas, including n=7 IDH wild-type (IDHwt) n=9...
Abstract BACKGROUND Central nervous system (CNS) tumors are the most lethal category of tumors, particularly among children. Most commonly, first line treatment CNS is neurosurgical resection tumor. During this procedure a delicate balance must be struck between extent and risk comorbidity. Ideally, surgical plan based on detailed classification However often unknown at start surgery. Current practice consists preoperative imaging intraoperative diagnosis achieved by rapid histological...
Abstract Human cortical pyramidal neurons are large, have extensive dendritic trees, and yet surprisingly fast input-output properties: rapid subthreshold synaptic membrane potential changes reliably encoded in timing of action potentials (APs). Here, we tested whether biophysical properties voltage-gated sodium (Na + ) potassium (K currents human can explain their properties. Na K exhibited more depolarized voltage-dependence, slower inactivation faster recovery from compared with mouse...
Abstract Intratumoral heterogeneity is a hallmark of diffuse gliomas. We used neuronavigation to acquire 133 image-guided and spatially-separated stereotactic biopsy samples from 16 adult patients with glioma, which we characterized using DNA methylation arrays. Samples were obtained regions without imaging abnormalities. Methylation profiles analyzed devise three-dimensional reconstruction genetic epigenetic heterogeneity. Molecular aberrations indicated that tumor was found outside...