A5017455465- Gut microbiota and health
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- Microbial Community Ecology and Physiology
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- COVID-19 Clinical Research Studies
- Bacterial Infections and Vaccines
- Inflammatory Bowel Disease
- Immune responses and vaccinations
- Salivary Gland Disorders and Functions
- Dermatology and Skin Diseases
- Mass Spectrometry Techniques and Applications
- Immune Cell Function and Interaction
- Eosinophilic Esophagitis
- T-cell and B-cell Immunology
- Gastrointestinal motility and disorders
- Barrier Structure and Function Studies
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Protein Structure and Dynamics
- Advanced Biosensing Techniques and Applications
- Adolescent and Pediatric Healthcare
- Microscopic Colitis
Technische Universität Berlin
2019-2025
German Rheumatism Research Centre
2019-2025
Charité - Universitätsmedizin Berlin
2024
Leibniz Association
2020-2023
The pathogenesis of severe COVID-19 reflects an inefficient immune reaction to SARS-CoV-2. Here we analyze, at the single cell level, plasmablasts egressed into blood study dynamics adaptive response in patients requiring intensive care. Before seroconversion SARS-CoV-2 spike protein, peripheral display a type 1 interferon-induced gene expression signature; however, following seroconversion, lose this signature, express instead signatures induced by IL-21 and TGF-β, produce mostly IgG1 IgA1....
The field of structural biology is increasingly focusing on studying proteins in situ, i.e., their greater biological context. Cross-linking mass spectrometry (CLMS) contributing to this effort, typically through the use (MS)-cleavable cross-linkers. Here, we apply popular noncleavable cross-linker disuccinimidyl suberate (DSS) human mitochondria and identify 5518 distance restraints between protein residues. Each restraint or interactions provides information within mitochondria. Comparing...
IgA-coated fractions of the intestinal microbiota Crohn's disease (CD) patients have been shown to contain taxa that hallmark compositional dysbiosis in CD microbiomes. However, correlation between other cellular properties bacteria and has not explored further, especially for features are directly driven by host immune-system, e.g. expression surface sugars bacteria. By sorting sequencing lectin-stained from healthy controls, we found were distinct bacteria, but still displayed specific...
The commensal microflora provides a repertoire of antigens that illicit mucosal antibodies. In some cases, these antibodies can cross-react with host proteins, inducing autoimmunity, or other microbial antigens. We demonstrate the oral microbiota induce salivary anti-SARS-CoV-2 Spike IgG via molecular mimicry. Anti-Spike in saliva correlated enhanced abundance Streptococcus salivarius 1 month after vaccination. Several human bacteria, including S. salivarius, were recognized by...
Polystyrene beads are broadly applied in flow cytometry. Implementing bead-based assays mass cytometry is desired but hampered by the lack of an elemental label required for their detection. In this study, we introduce stable osmium tetroxide labeling as a universal approach generating functionalized readily detectable We demonstrate utility osmium-labeled signal spillover compensation cytometry, and, strikingly, application quantitative Ab-binding capacity combined with high-dimensional...
Juvenile Idiopathic Arthritis (JIA) comprises diverse chronic inflammatory conditions driven by malfunction of the immune system. The intestinal microbiota is considered a crucial environmental factor correlating with diseases, and for JIA certain alterations in have already been described.
Abstract Receptor occupancy, the ratio between amount of drug bound and total receptor on single cells, is a biomarker for treatment response to therapeutic monoclonal antibodies. occupancy traditionally measured by flow cytometry. However, spectral overlap in cytometry limits number markers that can be simultaneously. This restricts assays analysis major cell types, although rare populations are potential relevance. We therefore developed assay suitable mass Measuring more than currently...
Short-read 16 S rRNA gene sequencing is the dominating technology to profile microbial communities in different habitats. Its uncontested taxonomic resolution paved way for major contributions field. Sample measurement and analysis, that is, sequencing, rather slow-in order of days. Alternatively, flow cytometry can be used microbiota various sources within a few minutes per sample. To keep up with high speed, we developed open source-analyzing tool FlowSoFine. validate ability distinguish...
Abstract Here we have analyzed the dynamics of adaptive immune response triggered by SARS-CoV-2 in severely affected COVID-19 patients, as reflected activated B cells egressing into blood, at single cell level. Early on, before seroconversion to spike protein, peripheral displayed a type 1 interferon-induced gene expression signature. After seroconversion, lost this signature, expressed IL-21- and TGF-β-induced signatures, mostly IgG1 IgA1. In sustained reaction until day 59, shifted IgA2,...
Abstract The commensal microflora is a source for multiple antigens that may induce cross-reactive antibodies against host proteins and pathogens. However, whether bacteria can SARS-CoV-2 remains unknown. Here we report several contribute to the generation of IgA receptor-binding domain (RBD) Spike protein. We identified unexposed individuals with RBD-binding at their mucosal surfaces. Conversely, neutralising monoclonal anti-RBD recognised distinct bacterial species. Some these bacteria,...
ZUSAMMENFASSUNG Entzündungsprozesse über die Ernährung zu regulieren, birgt das attraktive Potenzial, medikamentöse Behandlungen unterstützen oder deren Reduktion ermöglichen, Entzündungsschübe unterbinden und Familien der Betroffenen in Behandlung integrieren. Dabei besteht derzeit vor allem Chance, mittels Darmflora Patient*innen beeinflussen, im Fokus. Die hat einen besonderen Einfluss auf menschliche Gesundheit, da sie durch ständige Interaktion mit dem Immunsystem Darm Immunantworten...