A5037783602- Gut microbiota and health
- Pharmaceutical and Antibiotic Environmental Impacts
- Effects and risks of endocrine disrupting chemicals
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Probiotics and Fermented Foods
- Digestive system and related health
- Clostridium difficile and Clostridium perfringens research
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Toxic Organic Pollutants Impact
- Protein Hydrolysis and Bioactive Peptides
- Cytomegalovirus and herpesvirus research
- Drug Transport and Resistance Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Clinical Nutrition and Gastroenterology
- Silk-based biomaterials and applications
- Microscopic Colitis
- Agriculture Sustainability and Environmental Impact
- Microplastics and Plastic Pollution
- Air Quality and Health Impacts
- Acute Lymphoblastic Leukemia research
- Ginseng Biological Effects and Applications
German Rheumatism Research Centre
2021-2025
Helmholtz Centre for Environmental Research
2019-2024
Research Center Borstel - Leibniz Lung Center
2013
HAW Hamburg
2013
Universität Hamburg
2013
IgA-coated fractions of the intestinal microbiota Crohn's disease (CD) patients have been shown to contain taxa that hallmark compositional dysbiosis in CD microbiomes. However, correlation between other cellular properties bacteria and has not explored further, especially for features are directly driven by host immune-system, e.g. expression surface sugars bacteria. By sorting sequencing lectin-stained from healthy controls, we found were distinct bacteria, but still displayed specific...
Many functions in host–microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on composition and functionality gut microbiota. To analyze these effects, we cultivated model community SIHUMIx bioreactors order to determine varying (TT) structure function. After five days continuous cultivation, investigated influence different medium TT 12 h, 24 48 h. For profiling microbial community, applied flow...
Diverse intestinal microbiota is frequently used in vitro bioreactor models to study the effects of diet, chemical contaminations, or medication. However, reproducible cultivation fecal challenging and resultant communities behave highly dynamic. To approach issue reproducibility models, we established an model community reduced complexity, SIHUMIx, as a valuable for use. The development SIHUMIx was monitored over time with methods covering cellular molecular level. We microbial flow...
Glyphosate is the world's most widely used herbicide, and its potential side effects on intestinal microbiota of various animals, from honeybees to livestock humans, are currently under discussion. Pigs among abundant animals worldwide an impact glyphosate their function can have serious consequences health, not mention economic effects. Recent studies that addressed microbiota-disrupting focused microbial taxonomy but lacked functional information. Therefore, we chose experimental design...
The prevalence of inflammatory bowel disease (IBD) is rising globally; however, its etiology still not fully understood. Patient genetics, immune system, and intestinal microbiota are considered critical factors contributing to IBD. Preclinical animal models crucial better understand the importance individual factors. Among these, dextran sodium sulfate (DSS) colitis model most widely used. DSS treatment induces gut inflammation dysbiosis. However, exact mode action remains unclear. To...
Mucosal-associated invariant T-cells (MAIT) can react to metabolites of the vitamins riboflavin and folate which are produced by human gut microbiota. Since several studies showed that pesticide chlorpyrifos (CPF) glyphosate (GLP) impair microbiota, present study was undertaken investigate impact CPF GLP treatment on metabolism microbiota resulting bacteria-mediated modulation MAIT cell activity. Here, Bifidobacterium adolescentis (B. adolescentis), Lactobacillus reuteri (L. reuteri),...
Recent research has demonstrated that MAIT cells are activated by individual bacterial or yeasts species possess the riboflavin biosynthesis pathway. However, little is known about cell activating potential of microbial communities and contribution community members. Here, we analyze a human intestinal model (SIHUMIx) as well microbiota after bioreactor cultivation. We determined SIHUMIx members to investigated whether stress can influence their potential. The was directly related relative...
Oral uptake is the primary route of human bisphenol exposure, resulting in an exposure intestinal microbiota and intestine-associated immune cells. Therefore, we compared impact A (BPA), F (BPF) S (BPS) on (i) microbiota, (ii) microbiota-mediated immunomodulatory effects (iii) direct mucosal-associated invariant T (MAIT) cells vitro. We acutely exposed fecal Bacteroides thetaiotaomicron Escherichia coli to BPA its analogues BPF BPS referring European tolerable daily intake (TDI), i.e. 2.3...
Bisphenol S (BPS) is an industrial chemical used in the process of polymerization polycarbonate plastics and epoxy resins thus can be found various plastic products thermal papers. The microbiota disrupting effect BPS on community structure microbiome has already been reported, but little known how affects bacterial activity function. To analyze these effects, we cultivated simplified human intestinal (SIHUMIx) bioreactors at a concentration 45 µM BPS. By determining biomass, growth SIHUMIx...
Traditional kefir, which is claimed for health-promoting properties, made from natural grain-based while commercial kefirs are of defined mixtures microorganisms. Here, approaches described how to discriminate and traditional kefirs. These two groups were characterized by in-depth analysis on the taxonomic functional level. Cultivation-independent targeted qPCR as well next-generation sequencing (NGS) proved a completely different microbial composition in While kefirs, Lactobacillus...
Glyphosate is one of the most widely used herbicides and it suspected to affect intestinal microbiota through inhibition aromatic amino acid synthesis via shikimate pathway. In vitro microbiome bioreactors are increasingly as model systems investigate effects on consequently methods for quantitation glyphosate its degradation product aminomethylphosphonic (AMPA) in required.An optimized protocol enables analysis both AMPA by simple extraction with methanol:acetonitrile:water (2:3:1) without...
One route of human exposure to environmental chemicals is oral uptake. This primarily true for that may leach from food packaging materials, such as bisphenols and phthalate esters. Upon ingestion, these compounds are transported along the intestinal tract, where they can be taken up into blood stream or distributed mucosal sites. At sites, immune cells in peripheral exposed potentially modulating cell functions. In present study, we investigated impact three common two esters on...
Equine sarcoid is a topically accessible model for the evaluation of anticancer peptides acting by physical membrane disruption avoiding complexity systemic application. We aim at evaluating and improving natural host defence as lead structures, where we focus on cationic amphipathic peptide NK‐2. Cytotoxicity tests, fluorescence microscopy chip‐based biosensor, which enabled real‐time monitoring cell metabolism, were applied. Cancer killing was dynamic with an initial phase increased...
Human health and the human microbiome are inevitably intertwined, increasing their relevance in clinical research. However, collection, transportation storage of faecal samples may introduce bias due to methodological differences, especially since postal shipping is a common practise large-scale cohort studies. Using four different Omics layer, we determined structural (16S rRNA sequencing, cytometric microbiota profiling) functional integrity (SCFAs, global metabolome) relation...