Abstract Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease affecting upper and lower motor neurons. Dysfunction death of neurons are closely related to the modified astrocytic environment. Astrocytic endfeet, lining blood–brain barrier (BBB), enriched in two proteins, aquaporin‐4 (AQP4) inwardly rectifying potassium channel (Kir) 4.1. Both channels important for maintainance functional BBB lining. In this study, expression levels AQP4 Kir4.1 were first time...

The acid-sensitive ion channel 1 (ASIC1α or BNaC2a) is the most abundant of all mammalian proton-gated channels and one that has broadest distribution in nervous system. Hallmarks ASIC1α are gating by external protons rapid desensitization. In sensory neurons ASIC1 may constitute a nociceptor for pain induced local acidification, whereas central it modulate synaptic activity. To gain insight into functional roles ASIC1, we cloned examined properties evolutionarily distant species toadfish...

Two newly synthesized 4‐hydroxycoumarin bidentate ligands (L1 and L2) their palladium(II) complexes (C1 C2) were screened for biological activities, in vitro vivo . Structures of new compounds established based on elemental analysis, 1 H NMR, 13 C IR spectroscopic techniques. The obtained tested antioxidative cytotoxic activities results pointed to selective antiradical activity towards • OH -• OOH radicals anti‐ABTS (2,2 ′ ‐Azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) cation radical)...

The serum- and glucocorticoid-induced kinase-1 (sgk1) increases the activity of a number epithelial ion channels transporters. present study examines distribution subcellular localization sgk1 protein in rat kidney regulation levels expression induced by steroids. results indicate that expresses predominantly isoform with restricted to thick ascending limb Henle, distal convoluted, connecting cortical collecting tubules. Within cells, strongly associates microsomal fraction homogenates it...

Abstract We report the utilization of Third-Harmonic Generation microscopy for label-free live cell imaging lipid droplets in hypha filamentous fungus Phycomyces blakesleeanus . THG images showed bright spherical features dispersed throughout cytoplasm control conditions and a transient increase number after complete nitrogen starvation. Colocalization analysis lipid-counterstained disclosed that cytoplasmic particles were droplets. Particle Size Analysis Image Correlation Spectroscopy used...

In the present report, by using a patch clamp technique, we provide, to our knowledge, first detailed description of an anionic channel from filamentous fungi. The characterized channel, outwardly rectifying (ORAC), is most prominent feature cell membrane fungus Phycomyces blakesleeanus in absence energizing substrates. unitary conductance 11.3 +/- 0.4 pS. It strong voltage dependence open-channel probability (zdelta; gating charge 2.1 0.1), and activated depolarization. values time...

The effect of extremely low frequency magnetic fields (50 Hz, 0.5 mT) -ELF-MF, on phosphate metabolism has been studied in the isolated ganglions garden snail Helix pomatia, after 7 and 16 days exposure to ELF-MF.The influence ELF-MF level compounds intracellular pH was monitored by 31 P NMR spectroscopy.Furthermore, activity enzymes involved turnover, total ATPases, Na + /K -ATPase acid phosphatase measured.The snails for period shifted toward more alkaline conditions, increased...

Increasing resistance of fungal strains to known fungicides has prompted identification new candidates for among substances previously used other purposes. We have tested the effects anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic (NFA) on growth, energy metabolism anionic current mycelium fungus Phycomyces blakesleeanus. Both significantly decreased growth respiration mycelium, but complete inhibition was only achieved by 100 500 µM NFA respiration, respectively....

A bstract : Fish ASIC1 (fASIC1) cloned from Opsanus tau , unlike the rat ASICs, requires Ca 2+ in extracellular preconditioning solution (pH 7.4) to be activated. Here we show that fASIC1 is interacting with same way as mammalian ASICs: increasing proportion of channels available for activation by stabilizing closed state channel; process released; H + compete binding site making gating mechanism both and dependent; stabilizes desensitized state; blocks affinity block also modulated . The...

Studies of ion currents in filamentous fungi are a prerequisite for forming complete understanding their physiology. Cytoplasmic droplets (CDs), obtained from sporangiophores Phycomyces blakesleeanus, model system that enables the characterization native membrane, including mediated by channels not yet molecularly identified. Osmotically activated anionic current with outward rectification (ORIC) is dominant membrane cytoplasmic under conditions hypoosmotic stimulation. We have previously...

Abstract Studying the membrane physiology of filamentous fungi is key to understanding their interactions with environment and crucial for developing new therapeutic strategies disease-causing pathogens. However, plasma has been inaccessible a micron-sized patch-clamp pipette pA current recordings due rigid chitinous cell wall. Here, we report first femtosecond IR laser nanosurgery wall fungi, which enabled measurements on protoplasts released from hyphae. A reproducible highly precise...

In the photosensitive MB neuron in left parietal ganglion of Helix pomatia, onset light prolongs significantly (by about 40%) duration action potential. The broadening potential after was found to be due its calcium component and could not induced blocking Ca(2+) channels by Cd(2+) Pb(2+) absence medium. effect both compounds reversible. It that CdCl(2) exhibited a more intense than PbCl(2).

Abstract Ion channels are vital components of filamentous fungi signaling in communication with their environment. We exploited the ability apical region growing sporangiophores Phycomyces blakesleeanus to form membrane-enveloped cytoplasmic droplets (CDs), examine ion currents native plasma membrane. In hypoosmotic conditions, dominant current CDs is ORIC, an osmotically activated, anionic, outwardly rectified, fast inactivating instantaneous that we have previously characterized. Here,...

Abstract Ion channels are vital components of filamentous fungi signaling in communication with their environment. We exploited the ability apical region growing sporangiophores Phycomyces blakesleeanus to form membrane-enveloped cytoplasmic droplets (CDs), examine ion currents native plasma membrane. In hypoosmotic conditions, dominant current CDs is ORIC, an osmotically activated, anionic, outwardly rectified, fast inactivating instantaneous that we have previously characterized. Here,...

Abstract Studying the membrane physiology of filamentous fungi is key to understanding their interactions with environment and crucial for developing new therapeutic strategies disease-causing pathogens. However, plasma has been inaccessible micron sized patch-clamp pipette pA currents recordings due rigid chitinous cell wall. We report here first femtosecond IR laser nanosurgery wall fungi, enabling measurements on protoplasts released from hyphae. A reproducible highly precise (diffraction...

The success of patch-clamp giga-ohm seal formation on filamentous fungi Phycomyces blakesleeanus protoplasts was investigated to evaluate their usefulness in ion channel studies fungi. Protoplasts were obtained by laser-mediated nanosurgery the cell wall stained with Calcofluor White. To enable a successful formation, it is critical prevent regeneration. Since integrity responses involve kinase-dependent pathways, we aimed sufficiently reduce intracellular ATP availability using respiratory...

Adaptive changes in mitochondrial morphology are associated with the function and metabolic fitness of eukaryotic cells. We previously described young hyphae filamentous fungus Phycomyces blakesleeanus a dramatic effect an increase ambient temperature during growth: 3°C warmer environment compared control 22°C resulted appearance long elongated (“tubular”) mitochondria accompanied by lipid droplet density. Here, we examined how cooler (18°C) growth affects P. grown at 22°C. used two-photon...

Abstract We report the utilization of Third-Harmonic Generation (THG) microscopy, using home built nonlinear laser scanning microscope, for label-free live cell imaging lipid droplets in hypha filamentous fungus Phycomyces blakesleeanus . THG microscopy images show bright spherical features dispersed throughout cytoplasm control conditions and a transient increase number after complete nitrogen starvation. that predominant source signal imaged are test side by two methods quantification...

Unlike the neurons in CNS, peripheral have certain intrinsic regenerative capacity. After injury, can switch to a cellular “state for growth”, with expression profiles similar early developmental stages. We looked at changes of morphometric parameters induced young treatments that adult growth-stimulatory effect. The experimental compared control were: BpV (phen), an inhibitor PTEN; and bFGF, basic fibroblast growth factor. neurite was measured on cultured dissociated dorsal root ganglia...