A5057473795- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Immune responses and vaccinations
- Hematopoietic Stem Cell Transplantation
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Biosimilars and Bioanalytical Methods
- Immune Response and Inflammation
- Antimicrobial Resistance in Staphylococcus
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Chronic Lymphocytic Leukemia Research
Friedrich-Alexander-Universität Erlangen-Nürnberg
2020-2025
Universitätsklinikum Erlangen
2022
Paul Ehrlich Institut
2020
The pathogenesis of severe COVID-19 reflects an inefficient immune reaction to SARS-CoV-2. Here we analyze, at the single cell level, plasmablasts egressed into blood study dynamics adaptive response in patients requiring intensive care. Before seroconversion SARS-CoV-2 spike protein, peripheral display a type 1 interferon-induced gene expression signature; however, following seroconversion, lose this signature, express instead signatures induced by IL-21 and TGF-β, produce mostly IgG1 IgA1....
RNA vaccines are efficient preventive measures to combat the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. High levels of neutralizing SARS-CoV-2 antibodies an important component vaccine-induced immunity. Shortly after initial two mRNA vaccine doses, immunoglobulin G (IgG) response mainly consists proinflammatory subclasses IgG1 and IgG3. Here, we report that several months second vaccination, SARS-CoV-2–specific were increasingly composed noninflammatory IgG4,...
TRIANNI mice carry an entire set of human immunoglobulin V region gene segments and are a powerful tool to rapidly isolate monoclonal antibodies. After immunizing these with DNA encoding the spike protein SARS-CoV-2 boosting protein, we identified 29 hybridoma antibodies that reacted protein. Nine neutralize infection at IC50 values in subnanomolar range. ELISA-binding studies sequence analyses revealed one cluster three clonally related neutralizing target receptor-binding domain compete...
Abstract In clinical situations, peripheral blood accessible CD3 + CD4 CXCR5 T-follicular helper (T FH ) cells may have to serve as a surrogate indicator for dysregulated germinal center responses in tissues. To determine the heterogeneity of T versus tonsils, CD45RA – both origins were sorted. Transcriptomes, TCR repertoires and cell-surface protein expression analysed by single-cell RNA sequencing, flow cytometry immunohistochemistry. Reassuringly, all blood-circulating T-cell...
ABSTRACT TRIANNI mice carry an entire set of human immunoglobulin V region gene segments and are a powerful tool to rapidly generate monoclonal antibodies. After immunizing these against the spike protein SARS-CoV-2, we identified 29 hybridoma antibodies that reacted with SARS-CoV-2 protein. Nine neutralized infection at IC50 values in subnanomolar range. ELISA-binding studies DNA sequence analyses revealed one cluster clonally related neutralizing target receptor-binding domain compete...
Despite continuous exposure and development of specific immunity, Staphylococcus aureus (Sa) remains one the leading causes severe infections worldwide. Although innate immune defense mechanisms are well understood, role T cell response has not been fully elucidated. Here, we demonstrate that Sa its major virulence factors protein A (SpA) induce human regulatory cells (Tregs), key players in tolerance. In PBMC MoDC / co-cultures CD4+CD25+CD127dim Tregs were induced upon stimulation with to a...
Abstract Here we have analyzed the dynamics of adaptive immune response triggered by SARS-CoV-2 in severely affected COVID-19 patients, as reflected activated B cells egressing into blood, at single cell level. Early on, before seroconversion to spike protein, peripheral displayed a type 1 interferon-induced gene expression signature. After seroconversion, lost this signature, expressed IL-21- and TGF-β-induced signatures, mostly IgG1 IgA1. In sustained reaction until day 59, shifted IgA2,...
Abstract Repeated mRNA vaccinations are an efficient tool to combat the SARS-CoV-2 pandemic. High levels of neutralizing SARS-CoV-2-antibodies important component vaccine-induced immunity. Shortly after first or second vaccine dose, IgG response mainly consists pro-inflammatory isotypes IgG1 and IgG3 is driven by T helper (Th) 1 cells. Here, we report that several months vaccination, SARS-CoV-2-specific antibodies were increasingly composed non-inflammatory IgG2 particularly IgG4, which...
The germinal center (GC) reaction is crucial for somatic hypermutation, affinity maturation, and the selection of high-affinity B cells, all which are hallmarks humoral immune response. Understanding distinct roles various cell genes essential elucidating mechanisms within GC reaction. Traditionally, studying gene function in involved generating knock-out mice, a highly time-consuming method that necessitates complex vectors. advent Clustered Regularly Interspaced Short Palindromic Repeats...