A5010939557- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Trace Elements in Health
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Biosimilars and Bioanalytical Methods
- Chromatin Remodeling and Cancer
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Advanced Fluorescence Microscopy Techniques
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- CAR-T cell therapy research
Max Planck Institute for Molecular Biomedicine
2021-2024
Institute for Research in Biomedicine
2024
Centre for Genomic Regulation
2020-2022
Helmholtz Zentrum München
2020
Institut de Biologia Evolutiva
2016-2017
Universitat Pompeu Fabra
2016-2017
There is considerable interest in understanding the effect of transposable elements (TEs) on embryonic development. Studies humans and mice are limited by difficulty working with mammalian embryos relative scarcity active TEs these organisms. The zebrafish an outstanding model for study vertebrate development, over half its genome consists diverse TEs. However, remain poorly characterized. Here we describe demography genomic distribution their expression throughout embryogenesis using bulk...
Transition from maternal to embryonic transcriptional control is crucial for embryogenesis. However, alternative splicing regulation during this process remains understudied. Using transcriptomic data human, mouse, and cow preimplantation development, we show that the stage of zygotic genome activation (ZGA) exhibits highest levels exon skipping diversity reported any cell or tissue type. Much temporary, leads disruptive noncanonical isoforms, occurs in genes enriched DNA damage response...
Mutations in NR2E3, a gene encoding an orphan nuclear transcription factor, cause two retinal dystrophies with distinct phenotype, but the precise role of NR2E3 rod and cone transcriptional networks remains unclear. To dissect function, we performed scRNA-seq retinas wildtype different Nr2e3 mouse models that show phenotypes similar to patients carrying mutations. Our results reveal populations are not homogeneous can be separated into sub-classes. We identify previously unreported pathway...
Abstract Organisms must cope with altered environmental conditions such as high concentrations of heavy metals. Stress response to metals is mediated by the metal-responsive transcription factor 1 (MTF-1), which conserved from Drosophila humans. MTF-1 binds metal elements (MREs) and changes expression target genes. kuzbanian (kuz ), a metalloendopeptidase that activates evolutionary Notch signaling pathway, has been identified an gene. We have previously putatively adaptive transposable...
Abstract Immune checkpoint inhibition (ICI) has revolutionized oncology, offering extended survival and long-term remission in previously incurable cancers. While highly effective tumors with high mutational burden, lowly mutated cancers, including pediatric malignancies, present low response rate limited predictive biomarkers. Here, we a framework for the identification validation of tumor-reactive T cells as biomarker to quantify ICI efficacy candidates personalized TCR-T cell therapy....
SUMMARY Mutations in NR2E3 cause two retinal dystrophies with a distinct phenotype. encodes an orphan nuclear transcription factor that contributes to photoreceptor cell fate determination by repressing cone while activating rod genes. To dissect function, we performed scRNA-seq the retinas of wild type and different Nr2e3 mouse models show phenotypes similar patients carrying mutations. Our results reveal populations are not homogeneous can be separated into sub- classes. We identify...
Abstract There is considerable interest in understanding the effect of transposable elements (TEs) on embryonic development. Studies humans and mice are limited by difficulty working with mammalian embryos, relative scarcity active TEs these organisms. Zebrafish an outstanding model for study vertebrate development over half its genome consists diverse TEs. However, zebrafish remain poorly characterized. Here we describe demography genomic distribution their expression throughout...
ABSTRACT Organisms must cope with altered environmental conditions such as high concentrations of heavy metals. Stress response to metals is mediated by the metal-responsive transcription factor 1 (MTF-1), which conserved from Drosophila humans. MTF-1 binds metal elements (MREs) and changes expression target genes. kuzbanian (kuz) , a metalloendopeptidase that activates evolutionary Notch signaling pathway, has been identified an gene. We have previously putatively adaptive transposable...
ABSTRACT The transition from maternal to embryonic transcriptional control is a crucial step in embryogenesis. However, how alternative splicing regulated during this process and it contributes early development unknown. Using transcriptomic data pre-implantation stages of human, mouse cow, we show that the stage zygotic genome activation (ZGA) exhibits highest levels exon skipping diversity reported for any cell or tissue type. Interestingly, much temporary, leads disruptive non-canonical...