A5042606552- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- IL-33, ST2, and ILC Pathways
- Aldose Reductase and Taurine
- Immunotherapy and Immune Responses
- Biochemical and Molecular Research
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Religion, Society, and Development
- Glycogen Storage Diseases and Myoclonus
- Immune cells in cancer
- Metabolism and Genetic Disorders
- Lysosomal Storage Disorders Research
- Cancer, Hypoxia, and Metabolism
- NF-κB Signaling Pathways
- Cardiac Ischemia and Reperfusion
- Mitochondrial Function and Pathology
- Cell Image Analysis Techniques
Centre for Genomic Regulation
2018-2024
Universitat Pompeu Fabra
2012-2024
Centro Nacional de Análisis Genómico
2024
Barcelona Biomedical Research Park
2012
IRCCS Materno Infantile Burlo Garofolo
2009
Palatine tonsils are secondary lymphoid organs (SLOs) representing the first line of immunological defense against inhaled or ingested pathogens. We generated an atlas human tonsil composed >556,000 cells profiled across five different data modalities, including single-cell transcriptome, epigenome, proteome, and immune repertoire sequencing, as well spatial transcriptomics. This census identified 121 cell types states, defined developmental trajectories, enabled understanding functional...
Toll-like receptors (TLRs) engage networks of transcriptional regulators to induce genes essential for antimicrobial immunity. We report that NFAT5, previously characterized as an osmostress responsive factor, regulates the expression multiple TLR-induced in macrophages independently osmotic stress. NFAT5 was induction key gene Nos2 (inducible nitric oxide synthase [iNOS]) response low and high doses TLR agonists but is required Tnf Il6 mainly under mild stimulatory conditions, indicating...
Abstract Palatine tonsils are secondary lymphoid organs representing the first line of immunological defense against inhaled or ingested pathogens. Here, we present a comprehensive census cell types forming human tonsil by applying single-cell transcriptome, epigenome, proteome and adaptive immune repertoire sequencing as well spatial transcriptomics, resulting in an atlas >357,000 cells. We provide glossary 121 annotated states, disentangle gene regulatory mechanisms that drive cells...
Abstract The ability of innate immune cells to respond pathogen-associated molecular patterns across a wide range intensities is fundamental limit the spreading infections. Studies on transcription responses pathogen-activated TLRs have often used relatively high TLR ligand concentrations, and less known about their regulation under mild stimulatory conditions. We had shown that factor NFAT5 facilitates expression antipathogen genes stimulation conditions corresponding low pathogen loads. In...
Abstract Single-cell transcriptomics allows the identification of cellular types, subtypes and states through cell clustering. In this process, similar cells are grouped before determining co-expressed marker genes for phenotype inference. The performance computational tools is directly associated to their accuracy, but lack an optimal solution challenges a systematic method comparison. Moreover, phenotypes from different studies challenging integrate, due varying resolution, methodology...
Late-onset Pompe disease (LOPD) is a rare genetic disorder caused by the deficiency of acid alpha-glucosidase leading to progressive cellular dysfunction owing accumulation glycogen in lysosome. The mechanism relentless muscle damage (a classic manifestation disease) has been studied extensively analysing whole-muscle tissue; however, little, if anything, known about transcriptional heterogeneity among nuclei within multinucleated skeletal cells. This first report application single-nucleus...