A5105929697- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- Lysosomal Storage Disorders Research
- Molecular Biology Techniques and Applications
- Mitochondrial Function and Pathology
- Nanoparticle-Based Drug Delivery
- Glycosylation and Glycoproteins Research
- Nanoplatforms for cancer theranostics
- Cancer, Hypoxia, and Metabolism
- interferon and immune responses
- Photoacoustic and Ultrasonic Imaging
- Gold and Silver Nanoparticles Synthesis and Applications
- Advancements in Transdermal Drug Delivery
- Galectins and Cancer Biology
- Cancer Research and Treatments
- Endometrial and Cervical Cancer Treatments
- RNA Interference and Gene Delivery
- Systemic Sclerosis and Related Diseases
- Metabolism and Genetic Disorders
- Plant and Fungal Interactions Research
- Photodynamic Therapy Research Studies
- Calcium signaling and nucleotide metabolism
- Animal Disease Management and Epidemiology
IRCCS Materno Infantile Burlo Garofolo
2007-2024
University of Trieste
2013
Cervical cancer is one of the most common cancers affecting women worldwide. There are an estimated 570.000 new cases cervical each year and conventional treatments can cause severe side effects. In this work, we developed a platform for vaginal administration lipophilic drugs treatment. We formulated mucoadhesive cubosomes delivery curcumin, drug treatment, to increase its bioavailability local absorption. This study tests use assesses their potential efficiency using CAM (chick embryo...
In this work, iron/silica/gold core-shell nanoparticles (Fe3O4@SiO2@Au NPs) characterized by magnetic and optical properties have been synthesized to obtain a promising theranostic platform. To improve their biocompatibility, the obtained multilayer entrapped in polymeric micelles, decorated with folic acid moieties, tested vivo for photoacoustic resonance imaging detection of ovarian cancer.
Photodynamic therapy (PDT) is a potential synergistic approach to chemotherapy for treating ovarian cancer, the most lethal gynecologic malignancy. Here we used M13 bacteriophage as targeted vector efficient photodynamic killing of SKOV3 and COV362 cells. The phage was refactored (M13r) display an EGFR binding peptide in its tip that frequently overexpressed cancer. conjugated with chlorin e6 (Ce6), one widely photosensitizers (M13r-Ce6). new platform, upon irradiation, generated ROS by type...
The exact role of malignant ascites in the development intraperitoneal metastases remains unclear, and mechanisms by which extracellular vesicles (EVs) promote tumor progression pre-metastatic niche have not been fully discovered. In this study, we characterized from high-grade epithelial ovarian cancer patients. Small-EVs (30-150 nm) were isolated two sources-the bulk ascitic fluid-derived cell cultures-and assessed with a combination imaging, proteomic profiling, protein expression...
Endometriosis is a chronic inflammatory condition characterized by the presence of endometrium-like tissue outside uterus, primarily affecting pelvic organs and tissues. In this study, we explored platelet activation in endometriosis. We utilized STRING database to analyze functional interactions among proteins previously identified small extracellular vesicles (EVs) isolated from peritoneal fluid endometriosis patients controls. The bioinformatic analysis indicated enriched signaling...
Hypertrophic cardiomyopathy (HCM) is the most frequent autosomal dominant genetic heart muscle disease and common cause of sudden cardiac death in young people (under 30 y age), who are often unaware their underlying condition. Genetic screening now considered a fundamental tool for clinical management HCM families. However, high heterogeneity makes very expensive. Here, we propose new high-throughput genotyping method based on 96-well sequencing plate analysis 8 HCM-causing sarcomeric genes...
In ovarian cancer, ascites represent the microenvironment in which platelets extravasate to play their role disease progression. We aimed develop an assay measure ascites' platelet activation. enriched small extracellular vesicles (EVs) (40-200 nm) from of high-grade epithelial cancer patients (n = 12) using precipitation with polyethylene glycol, and we conducted single-particle phenotyping analysis by nano-flow cytometry after labelling ultra-centrifugation. Atomic force microscopy...
Chimerism status evaluation of post-allogeneic hematopoietic stem cell transplantation samples is essential to predict post-transplant relapse. The most commonly used technique capable detecting small increments chimerism quantitative real-time PCR. Although this method already in several laboratories, previously described protocols often lack sensitivity and the amount DNA required for each analysis too high. In present study, we compared a novel semi-nested allele-specific PCR (sNAS-qPCR)...
The coronavirus disease (COVID-19) is responsible for more than 5 million deaths worldwide, with respiratory failure being the most common clinical presentation. COVID-19 complications still present a considerable burden on healthcare systems, and signs of post-COVID syndrome are concerns potential long-term damages. An increasing body evidence highlights extracellular vesicles' (EVs) relevance in modulating inflammation cell death diseases related to these processes. Several types EVs-based...
In many mitochondrial diseases, different clinical manifestations are related to tissue-specific distribution of mutated DNA (mtDNA). this study, we describe an assay for the determination mtDNA copy number in small samples, using standard polymerase chain reaction (PCR) followed by SYBR Green real-time allelic-specific PCR [amplification refractory mutation system-quantitative (ARMS-qPCR)]. To assess degree heteroplasmy a patient harboring 2 cosegregating mutations (4415A>G and 9922A>C)...
Abstract Endometriosis is a chronic inflammatory condition characterized by the presence of endometrium-like tissue outside uterus, primarily affecting pelvic organs and tissues. In this study, we explored platelet activation in endometriosis. We utilized STRING database to analyze functional interactions among proteins previously identified small extracellular vesicles (EVs) isolated from peritoneal fluid endometriosis patients controls. The bioinformatic analysis indicated enriched...
In some cell systems, small extracellular vesicles bearing PD-L1 (PD-L1+ sEVs) have been shown to be able suppress T-cell immunity. We herein investigated whether a distinct profile of PD-L1+ sEVs exists in human follicular fluid (FF). Single-particle interferometric reflectance imaging sensing combined with single-particle antibody capture and immunofluorescence labelling were used determine the expression colocalization CD63, CD81, CD9, derived from FF women undergoing fertility treatments...
Abstract Endometriosis is a gynecological disease characterized by the presence of endometrial tissue in abnormal locations, leading to severe symptoms, inflammation, pain, organ dysfunction, and infertility. Surgical removal endometriosis lesions crucial for improving pain fertility outcomes, with goal complete lesion removal. Incomplete surgery often results recurring symptoms need additional interventions. This study aims explore biological significance PARP-1, EpCAM, FRα as potential...