A5004451013- DNA Repair Mechanisms
- Lipid Membrane Structure and Behavior
- Spectroscopy and Quantum Chemical Studies
- Plant Genetic and Mutation Studies
- Protein Structure and Dynamics
- DNA and Nucleic Acid Chemistry
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Microbial Metabolic Engineering and Bioproduction
- Cancer therapeutics and mechanisms
- Calcium signaling and nucleotide metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Sirtuins and Resveratrol in Medicine
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Historical and Contemporary Political Dynamics
- Parasitic Infections and Diagnostics
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
- Phytochemicals and Medicinal Plants
- Biotin and Related Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Adipose Tissue and Metabolism
Washington University in St. Louis
2021-2025
University of Iowa
2015-2019
Material Sciences (United States)
2010-2013
Kyoto University
2010-2012
Indian Institute of Science Bangalore
2003
Abstract Male C57BL/6J mice raised on high fat diet (HFD) become prediabetic and develop insulin resistance sensory neuropathy. The same given low doses of streptozotocin are a model type 2 diabetes (T2D), developing hyperglycemia, severe diabetic peripheral neuropathy involving motor neurons. Because suggestions that increased NAD + metabolism might address glycemic control be neuroprotective, we treated T2D with nicotinamide riboside (NR) added to HFD. NR improved glucose tolerance,...
Highlights•Postpartum liver circulates NAD metabolites to increase mammary NAD+ and NADP+ >20-fold•NR supplementation superinduces prolactin, biosynthetic programs, lactation•Weanlings of NR-fed mothers are hypoglycemia resistant advanced in motor learning•Adult offspring retain striking physical behavioral advantagesSummaryConditions metabolic stress dysregulate the metabolome. By restoring NAD, nicotinamide riboside (NR) provides resistance such conditions. We tested hypotheses that...
Interactions between membrane protein interfaces in lipid bilayers play an important role folding but quantification of the strength these interactions has been challenging. Studying dimerization ClC-type transporters offers a new approach to problem, as individual subunits adopt stable and functionally verifiable fold that constrains system two states – monomer or dimer. Here, we use single-molecule photobleaching analysis measure probability ClC-ec1 subunit capture into liposomes during...
UvrD-family helicases are superfamily 1A motor proteins that function during DNA replication, recombination, repair, and transcription. UvrD family monomers translocate along single-stranded (ss) but need to be activated by dimerization unwind in the absence of force or accessory factors. However, prior structural studies have only revealed monomeric complexes. Here, we report first structures a dimeric helicase, Mycobacterium tuberculosis UvrD1, both free bound junction. In each structure,...
Mycobacterium tuberculosis (Mtb) causes and, during infection, is exposed to reactive oxygen species and nitrogen intermediates from the host immune response that can cause DNA damage. UvrD-like proteins are involved in repair replication belong SF1 family of helicases use ATP hydrolysis catalyze unwinding. In Mtb, there two enzymes, where UvrD1 most closely related other members. Previous studies have suggested exclusively monomeric; however, it well known Escherichia coli UvrD members...
UvrD-family helicases are superfamily 1A motor proteins that function during DNA replication, recombination, repair, and transcription. UvrD family monomers translocate along single stranded (ss) but need to be activated by dimerization unwind in the absence of force or accessory factors. However, prior structural studies have only revealed monomeric complexes. Here, we report first structures a dimeric helicase,
UvrD-family helicases are superfamily 1A motor proteins that function during DNA replication, recombination, repair, and transcription. UvrD family monomers translocate along single stranded (ss) but need to be activated by dimerization unwind in the absence of force or accessory factors. However, prior structural studies have only revealed monomeric complexes. Here, we report first structures a dimeric helicase, _Mycobacterium tuberculosis_ UvrD1, both free bound junction. In each...
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In one-cell embryos of C. elegans, contraction an actomyosin network drives flows cell cortex from posterior to anterior.These anteriorly transport the small GTPase Rho, which is a positive regulator contractility.This raises question how feedback loop, between flow-driven concentration Rho and Rho-regulated contraction, prevented.We found that RhoGAP proteins RGA-3/4 colocalizes with actomyosin.Expression fusion RGA-3 GAP domain F-actin binding MOE rescued lethality rga-3/4 RNAi.These...
Abstract Mycobacterium tuberculosis is the causative agent of Tuberculosis. During host response to infection, bacterium exposed both reactive oxygen species and nitrogen intermediates that can cause DNA damage. It becoming clear damage in Mtb related actinobacteria function via distinct pathways as compared well-studied model bacteria. For example, we have previously shown repair helicase UvrD1 activated for processive unwinding redox-dependent dimerization. In addition, mycobacteria...