A5079354397- Immune Response and Inflammation
- Immune Cell Function and Interaction
- NF-κB Signaling Pathways
- Prostate Cancer Treatment and Research
- interferon and immune responses
- T-cell and B-cell Immunology
- Chromosomal and Genetic Variations
- TGF-β signaling in diseases
- Oil Palm Production and Sustainability
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- Renal and related cancers
- Epigenetics and DNA Methylation
- Gastric Cancer Management and Outcomes
- Ion channel regulation and function
- Metastasis and carcinoma case studies
- Tannin, Tannase and Anticancer Activities
- Colorectal Cancer Screening and Detection
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Radiomics and Machine Learning in Medical Imaging
- Nuclear Structure and Function
- Neuropeptides and Animal Physiology
- Ion Channels and Receptors
- Signaling Pathways in Disease
B2 (Slovenia)
2024-2025
University of Trento
2020-2022
AREA Science Park
2021
University of Trieste
2021
Helmholtz Zentrum München
2014-2016
Max Planck Society
2015
Max Planck Institute of Biochemistry
2010-2013
Abstract MALT1 channels proximal T-cell receptor (TCR) signalling to downstream pathways. With MALT1A and MALT1B two conserved splice variants exist we demonstrate here that alternative splicing supports optimal activation. Inclusion of exon7 in facilitates the recruitment TRAF6, which augments scaffolding function, but not protease activity. Naive CD4 + T cells express almost exclusively expression is induced by TCR stimulation. We identify hnRNP U as a suppressor inclusion. Whereas...
The substitution of one amino acid in the Roquin protein by sanroque mutation induces a dramatic autoimmune syndrome mice. This is believed to occur through ectopic expression inducible T cell co-stimulator (ICOS) and unrestrained differentiation follicular helper cells, which induce spontaneous germinal center reactions self-antigens. In this study, we demonstrate that tissue-specific ablation or B entire hematopoietic system, epithelial cells transplanted thymi did not cause autoimmunity....
Background: Colorectal cancer (CRC) is characterized by the uncontrolled growth of malignant colonic or rectal crypt epithelium. About 85% CRCs evolve through a stepwise progression from advanced precancerous adenoma lesions. A better understanding evolution to carcinoma can provide window opportunity not only for early detection and therapeutic intervention but potentially also prevention strategies. Methods: This study investigates heterogeneous methylation, copy-number alteration (CNA),...
Abstract Background: Advanced precancerous lesions (APL), precursors to colorectal cancer (CRC), progress from low-grade dysplasia (LGD) high-grade (HGD) through a stepwise accumulation of genomic alterations. Those alterations include mutations, structural variants, and altered methylation patterns, driving dysplastic cells carcinogenic transformation. Herein, we present comprehensive analysis differential DNA profiles classify severity in adenomas. Methods: Whole-genome sequencing...
Abstract Background: Colorectal cancer (CRC) progresses from normal tissue to advanced precancerous lesions (APLs) and ultimately malignancy, driven by stepwise genetic epigenetic alterations. A key challenge in managing CRC is the risk of overdiagnosis APLs, which can lead unnecessary interventions. Identifying molecular signatures that accurately distinguish between low-grade dysplasia (LGD) high-grade (HGD) crucial for optimizing assessment clinical decision-making. This study integrates...
Abstract The IκB kinase (IKK) complex acts as the gatekeeper of canonical NF-κB signaling, thereby regulating immunity, inflammation and cancer. It consists catalytic subunits IKKα IKKβ regulatory subunit NEMO/IKKγ. Here, we show that ubiquitin binding domain (UBAN) in NEMO is essential for IKK/NF-κB activation response to TNFα, but not IL-1β stimulation. By screening a natural compound library identified an anthraquinone derivative inhibitor NEMO-ubiquitin (iNUB). Using biochemical NMR...
Abstract Therapy resistance is a major roadblock in oncology. Exacerbation of molecular dysfunctions typical cancer cells have proven effective twisting oncogenic mechanisms to lethal conditions, thus offering new therapeutic avenues for treatment. Here, we demonstrate that selective agonists Transient Receptor Potential cation channel subfamily M member 8 (TRPM8), characteristic the prostate epithelium frequently overexpressed advanced stage III/IV cancers (PCa), sensitize therapy...
Article7 March 2022Open Access Source DataTransparent process Intra-epithelial non-canonical Activin A signaling safeguards prostate progenitor quiescence Francesco Cambuli Corresponding Author [email protected] The Armenise-Harvard Laboratory of Cancer Biology & Genetics, Department Cellular, Computational and Integrative (CIBIO), University Trento, Italy Medicine, Genetics Development, Urology, Systems Biology, Herbert Irving Comprehensive Center, Columbia Medical New York, NY, USA...
21q22.2–3 deletion is the most common copy number alteration in prostate cancer (PCa). The genomic rearrangement results androgen-dependent de novo expression of ETS-related gene (ERG) cells, a condition promoting tumor progression to advanced stages disease. Interestingly, ERG characterizes 5–30% precursor lesions – High Grade Prostatic Intraepithelial Neoplasia (HGPIN) - where its role remains unclear. Here, by combining organoids technology with Click-chemistry coupled Mass Spectrometry,...
Replication-deficient recombinant adenoviruses are potent vectors for the efficient transient expression of exogenous genes in resting immune cells. However, most leukocytes refractory to adenoviral transduction as they lack coxsackie/adenovirus receptor (CAR). To circumvent this obstacle, we generated R26/CAG-CARΔ1(StopF) (where R26 is ROSA26 and CAG CMV early enhancer/chicken β actin promoter) knock-in mouse line. This strain allows monitoring situ Cre recombinase activity through CARΔ1....
Abstract Background Over the last forty years, North America and Europe have witnessed a notable surge in cases of esophageal adenocarcinoma (EAC). This malignancy typically arises from Barrett esophagus (BE), condition characterized by replacement normal squamous epithelium with specialized metaplastic cells. Our study aims to identify specific methylation markers capable distinguishing between BE patients that will not progress EAC. Methods We conducted differential expression analysis on...
Abstract Liquid biopsies and cell-free DNA (cfDNA) analysis have emerged as novel non-invasive diagnostic tools in oncology. Analysis of cfDNA methylation status has been proposed promising approach for several purposes, robust methylation-detecting technologies are primary importance the clinical implementation assays. Signal-C is an accurate NGS-based methyl-sensitive assay detecting colorectal cancer (CRC) currently under validation. In this study we assessed influence different...
Background: Colorectal cancer (CRC) is characterized by uncontrolled growth of malignant colonic or rectal crypt epithelium. About 85% CRCs evolve through a stepwise progression from dysplastic colorectal adenomas. Better understanding the evolution adenoma to carcinoma can provide window opportunity not only for early detection and therapeutic intervention but potentially also prevention strategies. Methods: This study investigates heterogeneous methylation, copy number alteration (CNA),...
Abstract The healthy prostate is a relatively quiescent tissue. Yet, epithelium overgrowth common condition during ageing, associated with urinary dysfunction and tumorigenesis. For over thirty years, TGF-β ligands have been known to induce cytostasis in large variety of epithelia, but the intracellular pathway mediating this signal prostate, as well its relevance for quiescence, remained elusive. Here, using mouse organoids model epithelial progenitors, we found that intra-epithelial...