A5081891613- Nitric Oxide and Endothelin Effects
- Atherosclerosis and Cardiovascular Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hormonal Regulation and Hypertension
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Eicosanoids and Hypertension Pharmacology
- Cardiovascular Disease and Adiposity
- Angiogenesis and VEGF in Cancer
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Adipokines, Inflammation, and Metabolic Diseases
- MicroRNA in disease regulation
- Air Quality and Health Impacts
- Smoking Behavior and Cessation
- Genomics, phytochemicals, and oxidative stress
- Aortic aneurysm repair treatments
- Cardiovascular Health and Disease Prevention
- Renin-Angiotensin System Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Estrogen and related hormone effects
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Axon Guidance and Neuronal Signaling
- Antiplatelet Therapy and Cardiovascular Diseases
Technische Universität Dresden
2015-2024
University Hospital Carl Gustav Carus
2015-2024
Queen's University Belfast
2024
Janssen (Belgium)
2012-2018
King's College London
2016
Centro Cardiologico Monzino
2016
Institute of Experimental Cardiology
2016
Imperial College London
2016
University of Florence
2016
Czech Academy of Sciences
2012
Endothelial dysfunction is an early step in the development of atherosclerosis. Increased formation superoxide anions by NADPH oxidase Nox1, 2, and 5 reduces nitric oxide availability can promote endothelial dysfunction. In contrast, recent evidence supports a vasoprotective role H2O2 produced main isoform Nox4. Therefore, we analysed impact genetic deletion Nox4 on atherosclerosis low-density lipoprotein receptor (Ldlr) knockout model.Ex vivo analysis function Mulvany myograph showed...
Autophagy-driven nuclear transfer of miR-126-5p guided by Mex3a enables direct inhibition caspase-3 and protects against atherosclerosis.
The retina is prone to oxidative stress from many factors which are also involved in the pathogenesis of degenerative diseases. In this study, we used application blue light as a physiological factor. aim study was identify major source intracellular ROS that mediates light-induced detrimental effects on cells may lead cytotoxicity. We hypothesized outer segments induced generation. photoreceptors, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox) enzymes and recently found...
Tobacco smoking and hemodynamic forces are key stimuli for the development of endothelial dysfunction. As an alternative to smoking, next generation tobacco nicotine products (NGP) now widely used. However, little is known about their potential pro-inflammatory atherogenic effects on endothelium. In this study, we analyzed parameters function after exposure aqueous smoke extracts (AqE) a heated product (HTP), electronic cigarette (e-cig), conventional (3R4F) pure nicotine. All experiments...
Tobacco smoking and hemodynamic forces are key stimuli in the development of endothelial dysfunction atherosclerosis. High laminar flow has an atheroprotective effect on endothelium leads to a reduced response cells cardiovascular risk factors compared regions with disturbed or low flow. We hypothesize that high could delay caused by cigarette smoking. Primary human were stimulated increasing dosages aqueous smoke extract (CSEaq). CSEaq cell viability dose-dependent manner. The main mediator...
Abstract Aims Physical activity is one of the most potent strategies to prevent endothelial dysfunction. Recent evidence suggests vaso-protective properties hydrogen peroxide (H2O2) produced by main NADPH oxidase isoform 4 (Nox4) in vasculature. Therefore, we hypothesized that Nox4 connects physical with effects. Methods and results Analysis function using Mulvany Myograph showed dysfunction wild-type (WT) as well C57BL/6J/ Nox4−/− (Nox4−/−) mice after 20 weeks on high-fat diet (HFD). Access...
Obesity and type 2 diabetes have become a major public health problem worldwide. Steroid hormone dysfunction appears to be linked development of obesity correction steroid abnormalities may offer new approaches therapy. We therefore analyzed plasma steroids in 15–16 week old obese diabetic <i>db/db</i> mice using liquid chromatography-tandem mass spectrometry. Lean <i>db/+</i> served as controls. <i>Db/db</i> developed obesity, hyperglycemia, hyperleptinemia, hyperlipidemia. Hepatic...
Abstract Monocytes exhibiting a pro-inflammatory phenotype play key role in adhesion and development of atherosclerotic plaques. As an alternative to smoking, next-generation tobacco nicotine products (NGP) are now widely used. However, little is known about their effects on monocytes. We investigated cell viability, anti-oxidant gene protein expression THP-1 monocytes after exposure aqueous smoke extracts (AqE) heated product (HTP), electronic cigarette (e-cig), conventional (3R4F) pure...
Endothelin-1 is a key regulator of vascular tone and blood pressure in health disease. We have recently found that ET-1 production human microvascular endothelial cells (HMECs) can be promoted by angiotensin II (Ang II) through novel mechanism involving octamer-binding transcription factor-1 (Oct-1), NADPH oxidase-2 (NOX2), superoxide anions. As the formation bioactive also depends on endothelin-converting enzyme-1 (ECE-1), we investigated transcriptional regulation
Respiratory viruses continue to present serious health challenges human wellness. Growing evidence suggests that the more severe and damaging effects symptoms of influenza, rhinovirus (RV), respiratory syncytial virus (RSV), COVID-19 may primarily result from their common ability disorganize body’s healthy immune response. The simultaneous over-stimulation several reactive oxygen species (ROS) pathways concurrent suppression bioavailable Nitic Oxide (NO) contribute an disbalance can lead...
Increasing evidence suggests that superoxide ions produced by NOX (nicotinamide adenine dinucleotide phosphate oxidases) mediate vascular effects of Ang II (angiotensin II) evoked atherogenic diets. Here, we analyzed the mechanism which NOX2 contributes to II-induced ET-1 (endothelin 1) production in human microvascular endothelial cells.The high-fat diet were compared between WT (wild type) and Nox2 (mouse gene)-deficient mice. expression cells vitro ELISA, reverse transcription...
The NADPH oxidase NOX4 that releases H
Sphingosine-1-phosphate (S1P) has emerged as a potent bioactive lipid with multiple functions in cardiovascular pathophysiology. Potential roles of S1P heart valve diseases and expression relevant receptors (S1P1, S1P2, or S1P3) tissue valvular interstitial cells (VICs), the major cell population essential maintenance structure, are currently unknown. Exposure to (62–2000 nM) cultured VICs from porcine aortic valves on culture polystyrene resulted contraction nodule formation. The...
Cord blood-derived endothelial colony-forming cells (CB-ECFCs) are a defined progenitor population with established roles in vascular homeostasis and angiogenesis, which possess low immunogenicity high potential for allogeneic therapy highly sensitive to regulation by reactive oxygen species (ROS). The aim of this study was define the precise role major ROS-producing enzyme, NOX4 NADPH oxidase, CB-ECFC vasoreparative function.In vitro migration (scratch-wound assay) tubulogenesis (tube...
Plasma aldosterone is elevated in type 2 diabetes and obesity experimental clinical studies can act to inhibit both glucose-stimulated insulin secretion by the β-cell signaling. Currently mineralocorticoid receptor antagonism best characterized treatment ameliorate aldosterone-mediated effects. A second alternative inhibition of synthase, an approach with protective effects on end-organ damage heart or kidney animal models. The effect synthase metabolic parameters not known. Therefore, male...
Background Rupture of abdominal aortic aneurysm (rAAA) is associated with high case fatality rates, and risk rupture increases the AAA diameter. Heme oxygenase-1 (gene HMOX1, protein HO-1) a stress-induced induction has protective effects in vessel wall. HMOX1-/- mice are more susceptible to angiotensin II-induced formation, but regulation human nonruptured ruptured only poorly understood. Our hypothesis proposed that HO-1 reduced lowering inversely Methods Results walls from patients...