A5051876677- Protein Hydrolysis and Bioactive Peptides
- Biochemical effects in animals
- Consumer Attitudes and Food Labeling
- Insect Utilization and Effects
- RNA Research and Splicing
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Nutrition, Genetics, and Disease
- Protease and Inhibitor Mechanisms
- Neurogenetic and Muscular Disorders Research
- Renin-Angiotensin System Studies
- Pineapple and bromelain studies
- GDF15 and Related Biomarkers
- Amyotrophic Lateral Sclerosis Research
- Cardiac Ischemia and Reperfusion
- Plant Genetic and Mutation Studies
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Quantum Dots Synthesis And Properties
- Angiogenesis and VEGF in Cancer
- Blood Coagulation and Thrombosis Mechanisms
- Hormonal Regulation and Hypertension
- Lipoproteins and Cardiovascular Health
- Macrophage Migration Inhibitory Factor
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Mitochondrial Function and Pathology
Technische Universität Dresden
2008-2024
West Los Angeles College
2020
University Hospital Carl Gustav Carus
2015-2019
Universidad Nacional de La Plata
2010
Düsseldorf University Hospital
2008
Heinrich Heine University Düsseldorf
2005-2008
Abstract Aims Angiotensin‐converting enzyme inhibitors are treatment of choice in hypertensive patients. Clinically used exhibit a structural similarity to naturally occurring peptides. This study evaluated antihypertensive and cardioprotective effects ACE ‐inhibiting peptides derived from food proteins spontaneously rats. Methods Results Isoleucine–tryptophan ( vitro IC 50 for = 0.7 μ m ), whey protein hydrolysate containing an augmented fraction isoleucine–tryptophan, or captopril was...
The potential of hypoallergenic (HA) infant milk formulas containing hydrolyzed proteins as main constituents to inhibit angiotensin-converting enzyme (ACE) in vitro was investigated. Seven commercially available HA products designed for babies up 4 months showed a potent inhibition ACE vitro, with IC50 values ranging between 3.2 and 68.5 mg nitrogen/L. For six samples conventional milk-based three breast samples, no observed. Inhibitory did not correlate the degree hydrolysis. Using...
The generation of prostaglandin E2 (PGE2) is significantly increased in acute myocardial ischemia and reperfusion. PGE2, addition to other prostaglandins, protects the reperfused ischemic myocardium. It has been hypothesized that this cardioprotection mediated by E-type receptors Gi-coupled EP3 subtype.We tested hypothesis generating transgenic (tg) mice with cardiospecific overexpression receptor. According ligand binding, a 40-fold receptor was achieved membranes prepared from tg hearts...
AimsProstaglandin E2 (PGE2) has been shown to mediate anti-ischaemic effects and cardiomyocyte hypertrophy there is evidence for an involvement of the prostaglandin EP3-receptor subtype. This study focuses on EP3-mediated hypertrophic action investigates intracellular signalling pathways subtype in murine heart.
A new proteolytic preparation from Vasconcellea quercifolia ("oak leaved papaya") latex containing several cysteine endopeptidases with high activity has been obtained. The specific of the enzymatic (VQ) was higher than that Carica papaya latex. VQ able to coagulate milk and hydrolyze caseins then could be used produce cheeses and/or casein hydrolysates. Ion exchange chromatography allowed isolation a protease, named quercifoliain I, homogeneous when analyzed by SDS−PAGE, IEF MALDI-TOF-MS....
Plasma aldosterone is elevated in type 2 diabetes and obesity experimental clinical studies can act to inhibit both glucose-stimulated insulin secretion by the β-cell signaling. Currently mineralocorticoid receptor antagonism best characterized treatment ameliorate aldosterone-mediated effects. A second alternative inhibition of synthase, an approach with protective effects on end-organ damage heart or kidney animal models. The effect synthase metabolic parameters not known. Therefore, male...
Abstract Motoneurons are one of the highest energy demanding cell types and a primary target in Amyotrophic lateral sclerosis (ALS), debilitating lethal neurodegenerative disorder without currently available effective treatments. Disruption mitochondrial ultra-structure, transport metabolism is commonly reported phenotype ALS models can critically affect survival proper function motor neurons. However, how changes metabolic rates contribute to progression not fully understood yet. Here we...
Rats were fed a diet containing 0.3% (w/w) captan for 2, 4 and 12 weeks compared with controls, rats 0.02% N-2-acetylaminofluorene (AAF). Cell loss was evaluated from the drop of previously [14C] labeled DNA synthesis expressed as fractional incorporation [3H] dThd into hepatic testicular DNA. The feeding AAF produced in liver cell stimulation (+ 348%); testes, but an inhibition (- 84%) occurred. continuous no cytotoxic effect on increased between 2 100%). Then, induction seemed to reach...
Motoneurons are one of the most energy-demanding cell types and a primary target in Amyotrophic lateral sclerosis (ALS), debilitating lethal neurodegenerative disorder without currently available effective treatments. Disruption mitochondrial ultrastructure, transport, metabolism is commonly reported phenotype ALS models can critically affect survival proper function motor neurons. However, how changes metabolic rates contribute to progression not fully understood yet. Here, we utilize...
Objective: Angiotensin-Converting Enzyme (ACE)-inhibitors are treatment of choice in hypertensive patients. In former studies we revealed the ACE-inhibitory activity and antihypertensive effect natural dipeptide isoleucine-tryptophan (IW) its on cardiac remodelling coronary flow reserve spontaneously rats. The aim this project was to determine volunteers ACE-inhibiting bioavailability IW after oral intake whey hydrolysate. A second study influence combined IW-containing hydrolysate with...
Abstract Peptides with angiotensin‐converting enzyme (ACE)‐inhibitory and antihypertensive effects are suggested as innovative food additives to prevent or treat hypertension. Currently, these substances isolated from proteins following nonselective hydrolysis a mixture of ACE‐inhibitory peptides other protein fragments. This study presents an biotechnological method, based on recombinant DNA technology that was established specifically produce the dipeptide isoleucine‐tryptophan. In first...
Mitochondria are unique organelles that have their own genome (mtDNA) and perform various pivotal functions within a cell. Recently, evidence has highlighted the role of mitochondria in process stem cell differentiation, including differentiation neural cells (NSCs). Here we studied importance mtDNA function early NSCs two culture models: CGR8-NS line was derived from embryonic by lineage selection technique, primary were isolated day 14 mouse fetal forebrain. We detected dramatic increase...
Abstract Aims In the pathogenesis of several diseases, neo‐angiogenesis is increased (e.g. tumour growth). The peptide L‐glutamyl‐L‐tryptophan (EW/IM862) has been claimed to exhibit inhibitory effects on growth in vivo. However, potential role natural peptides with respect anti‐angiogenic properties unsettled. current study explores dipeptides WL, EW, IW and WE. Methods Results Using a bottom‐up strategy, we first evaluated VEGFR‐2 signalling quantified their different angiogenesis assays....
The present data are related to the research article entitled "Whey peptide isoleucine–tryptophan inhibits expression and activity of matrix metalloproteinase-2 in rat aorta" [1]. Here we on removal endothelium from aorta, dependent aortic relaxation inhibition pro-MMP2 by di-peptide (IW). Experiments were performed endothelial cells (EC) smooth muscle (SMC) vitro, along with isolated aorta ex vivo. stimulated angiotensin II (ANGII) or I (ANGI). ACE was inhibited treatment either IW...