A5082493852- PI3K/AKT/mTOR signaling in cancer
- Cellular transport and secretion
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Garlic and Onion Studies
- Ubiquitin and proteasome pathways
- Signaling Pathways in Disease
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Protein Kinase Regulation and GTPase Signaling
- Mitochondrial Function and Pathology
Kettering University
2024
University of Glasgow
2022-2023
Cancer Research UK Scotland Institute
2022-2023
Abstract Single cell profiling by genetic, proteomic and imaging methods has expanded the ability to identify programmes regulating distinct states. The 3-dimensional (3D) culture of cells or tissue fragments provides a system study how such states contribute multicellular morphogenesis. Whether plated into 3D cultures give rise singular phenotype whether multiple biologically phenotypes arise in parallel is largely unknown due lack tools detect heterogeneity. Here we develop Traject3d...
The glycocalyx component and sialomucin podocalyxin (PODXL) is required for normal tissue development by promoting apical membranes to form between cells, triggering lumen formation. Elevated PODXL expression also associated with metastasis poor clinical outcome in multiple tumor types. How presents this duality effect remains unknown. We identify an unexpected function of as a decoy receptor galectin-3 (GAL3), whereby the PODXL-GAL3 interaction releases GAL3 repression integrin-based...
ARF GTPases are central regulators of membrane trafficking that control local identity and remodeling facilitating vesicle formation. Unraveling their function is complicated by the overlapping association ARFs with guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), numerous interactors. Through a functional genomic screen three-dimensional (3D) prostate cancer cell behavior, we explore contribution GTPases, GEFs, GAPs, interactors to collective invasion. This...
Abstract Dysregulation of the PI3K/AKT pathway is a common occurrence in high‐grade serous ovarian carcinoma (HGSOC), with loss tumour suppressor PTEN HGSOC being associated poor prognosis. The cellular mechanisms how contributes to are largely unknown. We here utilise time‐lapse imaging spheroids coupled machine learning approach classify phenotype loss. deficiency induces PI(3,4,5)P 3 ‐rich and ‐dependent membrane protrusions into extracellular matrix (ECM), resulting collective invasion...
The mitochondrial genome, which encodes genes essential for respiration and cellular homeostasis, is the target of abundant highly diverse somatic alterations in cancers. Somatic to DNA (mtDNA) nearly always arise heteroplasmically, producing heterogeneous ensembles mtDNA within single cells. Here, we review new insights derived from exponential increases genomic sequencing data that have uncovered nature of, selective pressure for, functional consequences cancer-associated alterations. As...
Abstract Dysregulation of the PI3K/AKT pathway is a common occurrence in ovarian carcinomas. Loss tumour suppressor PTEN high-grade serous carcinoma (HGSOC) associated with patient subgroup poor prognosis. The cellular mechanisms how loss contributes to HGSOC are largely unknown. We utilise long-term time-lapse imaging spheroids coupled machine learning approach classify phenotype loss. deficiency does not affect proliferation but rather induces PI(3,4,5)P 3 -rich and -dependent membrane...
Abstract The glycocalyx component and sialomucin Podocalyxin (PODXL) is required for normal tissue development by promoting apical membranes to form between cells, triggering lumen formation. Elevated PODXL expression also associated with metastasis poor clinical outcome in multiple tumour types. How presents this duality effect remains unknown. We identify an unexpected function of as a decoy receptor Galectin-3 (GAL3), whereby the PODXL-GAL3 interaction releases GAL3 repression...