A5067948367- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Prostate Cancer Diagnosis and Treatment
- PARP inhibition in cancer therapy
- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Estrogen and related hormone effects
- Protein Degradation and Inhibitors
- Ferroptosis and cancer prognosis
- Urologic and reproductive health conditions
- Cancer Genomics and Diagnostics
- Amino Acid Enzymes and Metabolism
- Meta-analysis and systematic reviews
- Health Sciences Research and Education
- Molecular Biology Techniques and Applications
- Cancer Treatment and Pharmacology
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- Ubiquitin and proteasome pathways
- Diabetes Treatment and Management
- Colorectal Cancer Treatments and Studies
- Fibroblast Growth Factor Research
- Brain Metastases and Treatment
- Urinary Tract Infections Management
Queen Elizabeth University Hospital
2020-2023
University of Glasgow
2016-2023
Cancer Research UK Scotland Institute
2016-2023
NHS Greater Glasgow and Clyde
2014-2022
Cancer Research UK
2020-2022
Colchester Hospital
2012-2016
NIHR Cambridge Biomedical Research Centre
2015
University of Cambridge
2015
Gartnavel General Hospital
2014
Abstract Despite the clinical success of Androgen Receptor (AR)-targeted therapies, reactivation AR signalling remains main driver castration-resistant prostate cancer (CRPC) progression. In this study, we perform a comprehensive unbiased characterisation LNCaP cells chronically exposed to multiple inhibitors (ARI). Combined proteomics and metabolomics analyses implicate an acquired metabolic phenotype common in ARI-resistant associated with perturbed glucose lipid metabolism. To exploit...
Significance Using an unbiased forward mutagenesis screen, we were able to successfully identify candidate genes that drive advanced and metastatic prostate cancer (CaP). Alterations of peroxisome proliferator-activated receptor gamma ( PPARG ), encoding a regulator crucial lipid metabolism, appear play role in the development CaP both humans mice.
We report fundamental insights into the validity, reliability and clinical feasibility of using heterogeneous Pd catalysts as implantable devices to accurately activate chemotherapy within a tumour.
5-Fluorouracil (5-FU) is an antineoplastic antimetabolite that widely administered to cancer patients by bolus injection, especially those suffering from colorectal and pancreatic cancer. Because of its suboptimal route administration dose-limiting toxicities, diverse 5-FU prodrugs have been developed confer oral bioavailability increase the safety profile chemotherapy regimens. Our contribution this goal presented herein with development a novel palladium-activated prodrug designed evade...
Abstract Single cell profiling by genetic, proteomic and imaging methods has expanded the ability to identify programmes regulating distinct states. The 3-dimensional (3D) culture of cells or tissue fragments provides a system study how such states contribute multicellular morphogenesis. Whether plated into 3D cultures give rise singular phenotype whether multiple biologically phenotypes arise in parallel is largely unknown due lack tools detect heterogeneity. Here we develop Traject3d...
Histological assessment of baseline chronic kidney injury may discriminate kidneys that are suitable for transplantation, but has not been validated appraisal donation after circulatory death (DCD) kidneys. 'Time-zero' biopsies 371 consecutive, solitary, deceased-donor transplanted at our center between 2006 and 2010 (65.5% DCD, 34.5% brain [DBD]) were reviewed degenerative scored using Remuzzi's classification. High scores correlated with donor age extended criteria donors (42% donors), the...
Article11 January 2022Open Access Source DataTransparent process THEM6-mediated reprogramming of lipid metabolism supports treatment resistance in prostate cancer Arnaud Blomme Corresponding Author [email protected] orcid.org/0000-0003-4183-8726 CRUK Beatson Institute, Garscube Estate, Glasgow, UK Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing - original draft, Project administration, review & editing Search for more papers by this...
Abstract Androgen deprivation therapy (ADT) is the standard of care for treatment nonresectable prostate cancer. Despite high efficiency, most patients ultimately develop lethal castration-resistant cancer (CRPC). In this study, we performed a comparative proteomic analysis three in vivo, androgen receptor (AR)-responsive orthograft models matched hormone-naïve and CRPC. Differential revealed that distinct molecular mechanisms, including amino acid (AA) fatty metabolism, are involved...
BackgroundObesity and aggressive prostate cancer (PC) may be linked, but how local peri-prostatic fat relates to tumour response following androgen deprivation therapy (ADT) is unknown.ObjectiveTo test if volume (PPFV) predicts ADT.Design, setting, participantsWe performed a retrospective study on consecutive patients receiving primary ADT. From staging pelvic magnetic resonance imaging scans, the PPFV was quantified with OsirixX 6.5 software. Statistical (univariate multivariate) analysis...
Research Article14 March 2018Open Access Source DataTransparent process Sprouty2 loss-induced IL6 drives castration-resistant prostate cancer through scavenger receptor B1 Rachana Patel Corresponding Author [email protected] orcid.org/0000-0003-2147-4080 Cancer UK Beatson Institute, Glasgow, Search for more papers by this author Janis Fleming Ernest Mui Institute of Sciences, Carolyn Loveridge Peter Repiscak Arnaud Blomme Victoria Harle Mark Salji Imran Ahmad Katy Teo Freddie C Hamdy...
Abstract Background Prostate cancer is highly prevalent worldwide. Androgen deprivation therapy (ADT) remains the treatment of choice for incurable prostate cancer, but majority patients develop disease recurrence following ADT. There therefore an urgent need early detection resistance. Methods Isogenic androgen-responsive (CWR22Res) and castration-resistant (22Rv1) human cells were implanted into anterior lobes in CD-1 Nu mice to generate orthografts. Castrated bearing CWR22Res 22Rv1...
Salvage therapeutic options for biochemical failure after primary radiation-based therapy include radical prostatectomy, cryoablation, high-intensity focused ultrasound (HIFU), brachytherapy (for post-EBRT patients) and androgen deprivation (ADT). ADT salvage prostate cryoablation (SPC) are two commonly considered treatment RRPC. However, there is an urgent need high-quality clinical studies to support evidence-based decisions on choice. Our study aims determine the feasibility of...
There is a clinical need to improve assessment of biopsy-naïve patients for the presence clinically significant prostate cancer (PCa). In this study, we investigated whether robust integration expression data from urinary extracellular vesicle RNA (EV-RNA) with urine proteomic metabolites can accurately predict PCa biopsy outcome. Urine samples collected within Movember GAP1 Biomarker study (n = 192) were analysed by both mass spectrometry-based urine-proteomics and NanoString...
Abstract BRF1 is a rate-limiting factor for RNA Polymerase III-mediated transcription and elevated in numerous cancers. Here, we report that levels of associate with poor prognosis human prostate cancer. In vitro studies cancer cell lines demonstrated transient overexpression increased proliferation whereas the downregulation reduced mediated cycle arrest. Consistent our clinical observations, Pten -deficient mouse ( Δ/Δ Tg ) model accelerated carcinogenesis shortened survival. tumours,...
Extracellular signal-regulated protein kinase 5 (ERK5) has been implicated during development and carcinogenesis. Nkx3.1-mediated Cre expression is a useful strategy to genetically manipulate the mouse prostate. While grossly normal at birth, we observed an unexpected phenotype of spinal protrusion in Nkx3.1:Cre;Erk5
Prostate cancer (PCa) is the second most common tumour diagnosed in men. Tumoral heterogeneity PCa creates a significant challenge to develop robust prognostic markers and novel targets for therapy. An analysis of gene regulatory networks (GRNs) may provide insight into progressive PCa. Herein, we exploited graph-based enrichment score integrate data from GRNs identified preclinical prostate orthografts differentially expressed genes clinical resected We active regulons (transcriptional...
Pelvic nodal metastasis in prostate cancer impacts patient outcome negatively.
Castration-resistant prostate cancer (CRPC) is incurable and remains a significant worldwide challenge (Oakes Papa, 2015). Matched untargeted multi-level omic datasets may reveal biological changes driving CRPC, identifying novel biomarkers and/or therapeutic targets. Untargeted RNA sequencing, proteomics, metabolomics were performed on xenografts derived from three independent sets of hormone naive matched CRPC human cell line models local, lymph node, bone metastasis grown as murine...
To test for evidence of statin-mediated effects in patients with castration-resistant prostate cancer (CRPC) as post-diagnosis use statins is associated favourable survival outcome.
Abstract Background Risk of biochemical recurrence (BCR) in localised prostate cancer can be stratified using the 5‐tier Cambridge Prognostic Group (CPG) or 3‐tier European Association Urology (EAU) model. Active surveillance is current recommendation if CPG1 EAU low‐risk criteria are met. We aimed to determine contemporary rates upgrading, upstaging and BCR after radical prostatectomy for disease. Methods A database all robotic‐assisted laparoscopic prostatectomies (RALPs) performed Glasgow...
Prior to any large-scale basic science or clinical research project being funded, it is important for researchers gather preliminary data. This essential providing evidence the feasibility of projects and helping design larger-scale studies. When gathering data one needs consider how many are required, this work be funded where when will generated. Most importantly should ensure that planned collection meaningful, serve its intended purpose follow principles good practice.
INTRODUCTION The objective of the study was to compare bacterial fallout during vascular prosthesis insertion and orthopaedic major joint replacement performed in conventional laminar flow ventilation, respectively. MATERIALS AND METHODS A prospective single-centre case control 21 consecutive elective procedures involving prosthetic graft 24 replacements were tested for degree using agar settle plates. Preparation time, waiting time total procedure duration collected at surgery, colony...
Abstract Androgen-deprivation therapy (ADT) is the standard of care for treatment non-resectable prostate cancer (PCa). Despite high efficiency, most patients ultimately develop lethal castration-resistant (CRPC). In this study, we perform a comparative proteomic analysis three in vivo, androgen receptor (AR)–driven, orthograft models CRPC. Differential reveals that distinct molecular mechanisms, including amino acid (AA) and fatty (FA) metabolism, are involved response to ADT between...