A5100362677- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Heat shock proteins research
- RNA and protein synthesis mechanisms
- ATP Synthase and ATPases Research
- RNA Research and Splicing
- Plant Molecular Biology Research
- Enzyme Production and Characterization
- Plant Reproductive Biology
- Microbial Community Ecology and Physiology
- Plant biochemistry and biosynthesis
- Plant Pathogens and Fungal Diseases
- Biofuel production and bioconversion
- Tissue Engineering and Regenerative Medicine
- Advanced Cellulose Research Studies
- Protist diversity and phylogeny
- Vibrio bacteria research studies
- Plant Genetic and Mutation Studies
- Light effects on plants
- Probiotics and Fermented Foods
- Genomics and Rare Diseases
- Plant Water Relations and Carbon Dynamics
- Congenital heart defects research
- TGF-β signaling in diseases
- Plant responses to elevated CO2
Memorial Sloan Kettering Cancer Center
2023-2024
University of Massachusetts Amherst
2015-2022
Seoul National University
2003-2022
Gyeongsang National University
2022
Amherst College
2017
University of Arizona
2012-2016
University of Rochester
2013
Seoul National University Hospital
2012
Hallym University
2010
Flowering is regulated by an integrated network of several genetic pathways in Arabidopsis. The key genes integrating multiple flowering are FT, SOC1 and LFY. To elucidate the interactions among these integrators, analyses were performed. FT share common upstream regulators CO, a component long day pathway, FLC, repressor autonomous vernalization pathways. However, soc1 mutation further delayed time pathway mutants including ft, demonstrating that acts partially independently FT. Although...
In the nitrogen-fixing symbiosis between legume hosts and rhizobia, bacteria are engulfed by a plant cell membrane to become intracellular organelles. model Medicago truncatula, internalization differentiation of Sinorhizobium (also known as Ensifer) meliloti is prerequisite for nitrogen fixation. The host mechanisms that ensure long-term survival differentiating (bacteroids) in this unusual association unclear. M. truncatula defective fixation4 (dnf4) mutant unable form productive...
The ubiquitous small heat shock proteins (sHSPs) are well documented to act in vitro as molecular chaperones prevent the irreversible aggregation of heat-sensitive proteins. However, vivo activities sHSPs remain unclear. To investigate two most abundant classes plant cytosolic (class I [CI] and class II [CII]), RNA interference (RNAi) overexpression lines were created Arabidopsis (Arabidopsis thaliana) shown have reduced enhanced tolerance, respectively, extreme stress. Affinity purification...
Stressful environments often lead to protein unfolding and the formation of cytotoxic aggregates that can compromise cell survival. The molecular chaperone heat shock (HSP) 101 is a disaggregase co-operates with small HSP (sHSP) HSP70 chaperones facilitate removal such essential for surviving severe stress. To better define how HSP101 protects plants, we investigated localization targets this in Arabidopsis (Arabidopsis thaliana). By following tagged GFP, discovered its intracellular...
Abstract Flowering traits in winter annual Arabidopsis thaliana are conferred mainly by two genes, FRIGIDA (FRI) and FLOWERING LOCUS C (FLC). FLC acts as a flowering repressor is regulated multiple pathways. We isolated an early-flowering mutant, suppressor of FRIGIDA3 (suf3), which also shows leaf serration, weak apical dominance, infrequent conversion the inflorescence shoot to terminal flower. The suf3 mutation caused decrease transcript level both FRI-containing line autonomous pathway...
The molecular chaperone heat shock protein101 (HSP101) is required for acquired thermotolerance in plants and other organisms. To identify factors that interact with HSP101 or are involved thermotolerance, we screened extragenic suppressors of a dominant-negative allele Arabidopsis thaliana HSP101, hot1-4. One suppressor, shot1 (for suppressor hot1-4 1), encodes mitochondrial transcription termination factor (mTERF)–related protein, one 35 mTERFs about which there limited functional data....
Brain organoids have the potential to improve our understanding of brain development and neurological disease. Despite importance organoids, effect vascularization on is largely unknown. The objective this study develop vascularized by assembling vascular spheroids with cerebral organoids.In study, were generated from non-adherent microwell culture system human umbilical vein endothelial cells, dermal fibroblasts cord blood derived mesenchymal stem cells. These used for fusion iPSCs induced...
Gremlin-1, a bone morphogenetic protein (BMP) antagonist, is overexpressed in various cancerous tissues but its role carcinogenesis has not been established. Here, we report that gremlin-1 binds cancer cell lines and this interaction inhibited by our newly developed antibody, GRE1. Gremlin-1 binding to cells was unaffected the presence of BMP-2, BMP-4, BMP-7. In addition, independent vascular endothelial growth factor receptor-2 (VEGFR2) expression on surface. Addition A549 induced...
Mitochondria play critical roles in generating ATP through oxidative phosphorylation (OXPHOS) and produce both damaging signaling reactive oxygen species (ROS). They have reduced genomes that encode essential subunits of the OXPHOS machinery. Mitochondrial Transcription tERmination Factor-related (mTERF) proteins are involved organelle gene expression, interacting with organellar DNA or RNA. We previously found mutations Arabidopsis thaliana mTERF18/SHOT1 enable plants to better tolerate...
As rapid changes in climate threaten global crop yields, an understanding of plant heat stress tolerance is increasingly relevant. Heat involves the coordinated action many cellular processes and particularly energy demanding. We acquired a knockout mutant generated knockdown lines Arabidopsis thaliana d subunit mitochondrial ATP synthase (gene name: ATPQ, AT3G52300, referred to hereafter as ATPd), peripheral stalk, used these investigate phenotypic significance this normal growth tolerance....
The transmigration and extravasation of leukocytes across the endothelium that lines vessel wall occurs in distinct multisteps first comprising rolling over endothelial cells, resulting a tightly controlled very complex system leukocyte trafficking transmigration. Vascular cells are an important target proinflammatory cytokines modulating many genes involved cell adhesion, thrombosis, inflammatory responses. This study examined whether enzogenol blunts transendothelial migration monocytes...
It is well-established that plants are able to acclimate temperatures above or below the optimal temperature for their growth.Here, we provide protocols assays can be used quantitatively qualitatively assess relative ability of acquire tolerance high stress.The hypocotyl elongation assay described was developed screen mutants defective in acquisition extreme stress, and other were further characterize mutant transgenic heat processes at growth stages.Although details application Arabidopsis...
Dollar spot, caused by the ascomycete fungus Clarireedia (formerly Sclerotinia), is one of most resource-demanding diseases on amenity turfgrasses in North America. Differential resistance to succinate dehydrogenase inhibitor (SDHI) fungicide class, conferred singular point mutations SdhB, SdhC, and SdhD subunits enzyme (SDH), has been reported dollar spot as well many other plant-pathogenic fungal diseases. Four unique were previously from field isolates collected two different cool-season...
Despite recent advancements in our understanding of genetic etiology and its molecular physiological consequences, it is not yet clear what features determine the inheritance pattern a disease. To address this issue, we conducted whole exome sequencing analysis to characterize variants 1,180 Korean patients with neurological symptoms. The diagnostic yield for definitive pathogenic variant findings was 50.8%, after including 33 cases (5.9%) additionally diagnosed by reanalysis. Of patients,...
Heat shock protein 101 (HSP101) in plants, and bacterial yeast orthologs, is essential for thermotolerance. To investigate thermotolerance mechanisms involving HSP101, we performed a suppressor screen Arabidopsis thaliana of missense HSP101 allele (hot1-4). hot1-4 plants are sensitive to acclimation heat treatments that otherwise permissive null mutants, indicating the toxic. We report one (shot2, 2) has mutation conserved residue CLEAVAGE STIMULATION FACTOR77 (CstF77), subunit...
The MADS box gene, AGAMOUS-LIKE 20 (AGL20), integrates environmental and endogenous flowering signals in Arabidopsis thaliana. In order to determine if its role is conserved other plants, we isolated AGL20 orthologs from Brassica campestris, Cardamine flexuosa Draba nemorosa. putative amino acid sequences of the were 94 97% identical. We analyzed phenotype expression level ortholog C. flexuosa, a long day plant that does not respond vernalization. CaAGL20 was more highly expressed days than...
The mitochondrial genome, which encodes genes essential for respiration and cellular homeostasis, is the target of abundant highly diverse somatic alterations in cancers. Somatic to DNA (mtDNA) nearly always arise heteroplasmically, producing heterogeneous ensembles mtDNA within single cells. Here, we review new insights derived from exponential increases genomic sequencing data that have uncovered nature of, selective pressure for, functional consequences cancer-associated alterations. As...
Abstract Mitochondria play critical roles in eukaryotes ATP generation through oxidative phosphorylation (OXPHOS) and also produce both damaging signaling reactive oxygen species (ROS). Originating from endosymbiosis, mitochondria have their own reduced genomes that encode essential subunits of the OXPHOS machinery. MTERF (Mitochondrial Transcription tERmination Factor-related) proteins been shown to be involved organelle gene expression by interacting with organellar DNA or RNA...
Summary The mitochondrial genome encodes essential machinery for respiration and metabolic homeostasis but is paradoxically among the most common targets of somatic mutation in cancer genome, with truncating mutations respiratory complex I genes being over-represented 1 . While DNA (mtDNA) have been associated both improved worsened prognoses several tumour lineages 1–,3 , whether these are drivers or exert any functional effect on biology remains controversial. Here we discovered that...
Gamma-aminobutyric acid (GABA) can be converted from monosodium glutamate (MSG) by decarboxylase (GAD).GABA is known to a four-carbon non-protein amino existing in the human brain and eye.Recent studies have shown that GABA effective accelerating hypotension anxiety relief, along with acting as diuretic, antidiabetic, antidepressant humans other mammals.Since low activity of cell preparations has been major limitation commercial enzyme synthesis, it worthwhile look for methods obtain...
Abstract Background Heat Shock Protein 101 (HSP101) in plants and orthologs bacteria (Caseinolytic peptidase B, ClpB) yeast (Hsp104) are essential for thermotolerance. To investigate molecular mechanisms of thermotolerance involving HSP101, we performed a suppressor screen Arabidopsis thaliana semi-dominant, missense HSP101 allele, hot1-4 (A499T). Plants carrying the mutation more heat-sensitive than an null mutant ( hot1-3 ), indicating toxicity allele. Results We report that one shot2, 2 )...
Abstract Lysophosphatidic acid (LPA) has been suggested to regulate lymphocyte entry into lymph nodes because autotaxin (ATX), the enzyme that generates LPA, is constitutively expressed by node high endothelial venules. However very little known about effects of LPA on T cell migration and homing. We studied (16:0 18:1, 1-10 µM) naïve mouse CD4+ cells. Using chemotaxis assays, we found induces chemorepulsion (1.5±0.5 fold away from 1 µM n=26, p <0.0001) but not chemotaxis. In...
Cuttings based on water media are relatively low-cost and easy to manage compared those soil media. Nevertheless, species that root out in very few. Water-medium rooted cuttings not widely used. In media, the survival rate of is high, but many cases, they only form callus while rooting. If with formed a medium were survive after being transplanted soil, water-medium could be used as an important means propagating landscape tree species. this study, Ternstroemia japonica having various...