A5013588618- Heat shock proteins research
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Connexins and lens biology
- Chemotherapy-induced organ toxicity mitigation
- Liver Disease Diagnosis and Treatment
- Amyotrophic Lateral Sclerosis Research
- Yersinia bacterium, plague, ectoparasites research
- Bee Products Chemical Analysis
- Cellular transport and secretion
- Biochemical effects in animals
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Seaweed-derived Bioactive Compounds
- Chronic Kidney Disease and Diabetes
- Ziziphus Jujuba Studies and Applications
- Curcumin's Biomedical Applications
- RNA regulation and disease
- Chromatography in Natural Products
- Sperm and Testicular Function
- Neurological and metabolic disorders
- Paraoxonase enzyme and polymorphisms
- TGF-β signaling in diseases
- thermodynamics and calorimetric analyses
- Physiological and biochemical adaptations
Tanta University
2013-2025
University of Jordan
2025
University of Arizona
2004-2019
University of Massachusetts Amherst
2018
National Institutes of Health
2005
Small Heat Shock Proteins (sHSPs) are a diverse family of molecular chaperones that prevent protein aggregation by binding clients destabilized during cellular stress. Here we probe the architecture and dynamics complexes formed between an oligomeric sHSP client employing unique mass spectrometry strategies. We observe over 300 different stoichiometries interaction, demonstrating ensemble structures underlies protection these confer to unfolding clients. This astonishing heterogeneity not...
Small heat shock proteins (sHSPs) are a ubiquitous class of molecular chaperones that interacts with substrates to prevent their irreversible insolubilization during denaturation. How sHSPs interact remains poorly defined. To investigate the role conserved C-terminal alpha-crystallin domain versus variable N-terminal arm in substrate interactions, we compared two closely related dodecameric plant sHSPs, Hsp18.1 and Hsp16.9, four chimeras these which all or part was switched. The efficiency...
The ubiquitous small heat shock proteins (sHSPs) are well documented to act in vitro as molecular chaperones prevent the irreversible aggregation of heat-sensitive proteins. However, vivo activities sHSPs remain unclear. To investigate two most abundant classes plant cytosolic (class I [CI] and class II [CII]), RNA interference (RNAi) overexpression lines were created Arabidopsis (Arabidopsis thaliana) shown have reduced enhanced tolerance, respectively, extreme stress. Affinity purification...
The small heat shock proteins (sHSPs) are a ubiquitous class of ATP-independent chaperones believed to prevent irreversible protein aggregation and facilitate subsequent renaturation in cooperation with ATP-dependent chaperones. Although sHSP chaperone activity has been studied extensively vitro, understanding the mechanism function requires identification that substrates vivo. We have used both immunoprecipitation affinity chromatography recover 42 specifically interact Synechocystis...
The small heat shock proteins (sHSPs) and alpha-crystallins are highly effective, ATP-independent chaperones that can bind denaturing client to prevent their irreversible aggregation. One model of sHSP function suggests the oligomeric sHSPs activated client-binding form by dissociation at elevated temperatures dimers or other sub-oligomeric species. Here we examine this in a comparison structure chaperone activity two conserved classes cytosolic plants, class I (CI) II (CII) proteins. Like...
Oligomeric proteins assemble with exceptional selectivity, even in the presence of closely related proteins, to perform their cellular roles. We show that most by gene duplication an oligomeric ancestor have evolved avoid hetero-oligomerization and this correlates acquisition distinct functions. report how coassembly is avoided two small heat-shock protein paralogs. A hierarchy assembly, involving intermediates are populated only fleetingly at equilibrium, ensures selective oligomerization....
Small Hsps (sHsps) and the structurally related eye lens alpha-crystallins are ubiquitous stress proteins that exhibit ATP-independent molecular chaperone activity. We studied activity of dodecameric wheat TaHsp16.9C-I, a class I cytosolic sHsp from plants only eukaryotic for which high resolution structure is available, along with protein TaHsp17.8C-II, represents evolutionarily distinct II plant sHsps. Despite available structural information on there minimal data its activity, likewise,...
Small heat shock proteins (sHSPs) and the related alpha-crystallins are ubiquitous chaperones linked to neurodegenerative diseases, myopathies, cataract. To better define their mechanism of chaperone action, we used hydrogen/deuterium exchange mass spectrometry (HXMS) monitor conformational changes during complex formation between structurally defined sHSPs, pea PsHsp18.1, wheat TaHsp16.9, heat-denatured model substrates malate dehydrogenase (MDH) firefly luciferase. Remarkably, found that...
ABSTRACT Cyclophosphamide (CYP) is an extensively used immunosuppressive drug and chemotherapeutic agent for various malignancies. Nevertheless, its use limited due to adverse effects, including nephrotoxicity. Saxagliptin a DPP4 inhibitor, while cilostazol serves as antiplatelet agent. Their nephroprotective effects arise from antioxidant anti‐inflammatory properties. This study investigated the potential protective of Cilostazol in rats with kidney damage induced by CYP. Five equal groups...
To investigate the mechanism of small heat shock protein (sHsp) function, unbiased by current models sHsp chaperone activity, we performed a screen for mutations Synechocystis Hsp16.6 that reduced ability to provide thermotolerance in vivo . Missense at 17 positions throughout and C-terminal truncation 5 aa were identified, representing largest collection mutants impaired function Ten mutant proteins purified tested alterations native oligomeric structure vitro activity. These biochemical...
Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron degenerative disease. TDP-43 (TAR DNA-binding protein 43) and FUS (fused in sarcoma) are aggregation-prone RNA-binding proteins that ALS can mislocalize to the cytoplasm of affected cells, often forming cytoplasmic aggregates process. Such mislocalization aggregation implicated pathology, though mechanism(s) toxicity remains unclear. Recently, we determined endocytic function aids turnover (i.e., degradation) reduces toxicity. Here,...
The cardiotoxic effect of chemotherapeutic agents as cisplatin has become a major issue recently. Interference with mitochondrial dynamics, biogenesis, redox status, and apoptosis are the most possible underlying mechanisms. Semaglutide is human glucagon-like peptide-1 receptor agonist (GLP-1R), which used primarily for treatment DM. Various recent studies have investigated (GLP-1R) role in cardiovascular diseases due to antiapoptotic antioxidant effects. current study aimed investigate...
Abstract Growing evidence supports the role of gut-kidney axis and persistent mitochondrial dysfunction in pathogenesis diabetic nephropathy (DN). Ulinastatin (UTI) has a potent anti-inflammatory effect, protecting kidney gut barrier sepsis, but its effect on DN yet to be investigated. This study aimed assess potential mitigating UTI investigate possible involvement homeostasis this effect. Forty male Wistar rats were divided equally into four groups: normal; UTI-treated control; untreated...
Introduction. Vigabatrin (VGB) is an antiepileptic drug that acts to irreversibly inhibit the γ-aminobutyric acid (GABA) transaminase enzyme, elevating GABA levels. Broad studies have established long-term treatment and/or high doses of VGB lead variable visual defects. However, little attention has been paid its other side effects, especially those demonstrating cerebellar involvement. Sodium glucose-linked co-transporter 2 (SGLT2) inhibitors are antidiabetic agents with protective effects...
It is well-established that plants are able to acclimate temperatures above or below the optimal temperature for their growth.Here, we provide protocols assays can be used quantitatively qualitatively assess relative ability of acquire tolerance high stress.The hypocotyl elongation assay described was developed screen mutants defective in acquisition extreme stress, and other were further characterize mutant transgenic heat processes at growth stages.Although details application Arabidopsis...
This study aimed to examine the protective effect of celastrol on testicular dysfunction in diabetic rats and potential underlying mechanisms. All included were divided into four groups: a control group treated with sodium citrate buffer vehicle), celastrol-treated group, streptozotocin (STZ)-induced following insulin resistance, group. Serum glucose, triglyceride, total cholesterol, high-density lipoprotein interleukin (IL)-1β, tumor necrosis factor-alpha, testosterone levels measured. In...
Abstract Obesity is linked to reproductive disorders. Novel neuropeptide phoenixin demonstrated many therapeutic actions. In this study, we aim evaluate phoenixin’s potential effect in obesity-induced infertility through modulating mitochondrial dynamics. Ninety adult female rats were divided 4 groups: (I), fed with normal pellet diet; (II), given phoenixin; (III), high-fat diet. Rats that developed obesity and 2 (III-A), received no further treatment; (III-B), phoenixin. Our results showed...
The canonical function of small heat-shock proteins (sHSPs) is to interact with destabilized under conditions cellular stress. While the breadth interactions made by many sHSPs well-known, there currently little knowledge about what structural features interactors form basis for their recognition. Here, we have identified 83 in vivo sole sHSP cyanobacterium Synechocystis sp. PCC 6803, HSP16.6, reflective stable associations soluble conditions. By performing bioinformatic analyses on these...