Abstract Decellularized extracellular matrix scaffold, widely utilized for organ engineering, often undergoes decomposition after transplantation and produces byproducts that cause inflammation, leading to clinical failure. Here we propose a strategy using nano-graphene oxide modify the biophysical properties of decellularized liver scaffolds. Notably, demonstrate scaffolds crosslinked with show high resistance enzymatic degradation via direct inhibition metalloproteinase activity increased...

In cancer immunotherapy, chimeric antigen receptors (CARs) targeting specific antigens have become a powerful tool for cell-based therapy. CAR-natural killer (NK) cells offer selective anticancer lysis with reduced off-tumor toxicity compared to CAR-T cells, which is beneficial in the heterogeneous milieu of solid tumors. tumor microenvironment (TME) glioblastoma (GBM), pericytes not only support growth but also contribute immune evasion, underscoring their potential as therapeutic targets...

Abstract With the rapidly growing demand for mesenchymal stem cell (MSC) therapy, numerous strategies using MSCs different diseases have been studied and reported. Because of their immunosuppressive properties, are commonly used as an allogeneic treatment. However, many donors who could potentially be used, it is important to understand capacity therapeutic usage with donor-to-donor heterogeneity. In this study, we aimed investigate a promising strategy critical limb ischemia. We evaluated...

Brain organoids have the potential to improve our understanding of brain development and neurological disease. Despite importance organoids, effect vascularization on is largely unknown. The objective this study develop vascularized by assembling vascular spheroids with cerebral organoids.In study, were generated from non-adherent microwell culture system human umbilical vein endothelial cells, dermal fibroblasts cord blood derived mesenchymal stem cells. These used for fusion iPSCs induced...

A correlation between COVID-19 and Alzheimer's disease (AD) has been proposed recently. Although the number of case reports on neuroinflammation in patients increased, studies SARS-CoV-2 neurotrophic pathology using brain organoids have restricted recapitulation those phenotypes due to insufficiency immune cells absence vasculature. Cerebral pericytes endothelial cells, major components blood-brain barrier, express viral entry receptors for response systemic inflammation including direct...

Solar ultraviolet (sUV) exposure is known to cause skin damage. However, the pathological mechanisms of sUV on hair follicles have not been extensively explored. Here, we established a model sUV-exposed and its appendages using human induced pluripotent stem cell-derived organoids with planar morphology containing follicles. Our closely recapitulated several symptoms photodamage, including barrier disruption, extracellular matrix degradation, inflammatory response. Specifically, structural...

Brain organoids have emerged as a novel model system for neural development, neurodegenerative diseases, and human-based drug screening. However, the heterogeneous nature immature neuronal development of brain generated from pluripotent stem cells pose challenges. Moreover, there are no previous reports three-dimensional (3D) hypoxic injury cells. Here, we self-organized 3D human adult dermal fibroblast-derived Radial glial in these exhibited characteristics cerebral cortex trend, including...

Three-dimensional (3D) bioprinting is a promising technology to establish 3D in vitro hepatic model that holds great potential toxicological evaluation. However, current models, the central area suffers from hypoxic conditions, resulting slow and weak metabolism of drugs toxins. It remains challenging predict accurate drug effects bioprinted models. Here, we constructed hexagonal construct incorporated spinning condition with continuous media stimuli. Under HepG2 cells exhibited enhanced...

Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that characterized by loss of motor neurons and degeneration neuromuscular junctions. To improve progression, previous studies have suggested many options shown beneficial effects in diseases, especially stem cell therapy. In this study, we used repeated intramuscular transplantation human umbilical cord blood-derived mesenchymal cells (hUCB-MSCs) observed positive on muscle atrophy oxidative stress. an vivo...

Abstract The lymphatic vessel (LV) plays an important role in cancer biology as a major route for tumor metastasis. Whereas, recent oncoimmunological approaches have focused its immune surveillance. In response to the emerging topics of vascular biology, physiologically relevant human cell‐based vitro model is high demand. This study introduces 3D LV within microenvironment (TIME) using injection‐molded plastic array culture platform (Lymph‐IMPACT). Through spontaneous capillary flow‐driven...

Although the generation of ETV2-induced endothelial cells (iECs) from human fibroblasts serves as a novel therapeutic strategy in regenerative medicine, process is inefficient, resulting incomplete iEC angiogenesis. Therefore, we employed chromatin immunoprecipitation (ChIP) sequencing and identified molecular mechanisms underlying ETV2-mediated transdifferentiation to efficiently produce iECs retaining appropriate functionality long-term culture. We revealed that majority ETV2 targets are...

Abstract As modeling of atherosclerosis requires recapitulating complex interactions with vasculature and immune cells, previous in vitro models have limitations due to their insufficient 3D vascular structures. However, induced pluripotent stem cell‐derived blood vessel organoids (BVOs) are applicable for diseases, containing multiple cell types, including endothelial smooth muscle cells self‐assembled into a structure. Atherosclerotic BVOs microenvironment associated atherogenesis, such as...

Recent advances have shown the direct reprogramming of mouse and human fibroblasts into induced neural stem cells (iNSCs) without passing through an intermediate pluripotent state. Thus, strategy possibly provides a safe homogeneous cellular platform. However, applications iNSCs for regenerative medicine are limited by restricted availability cell sources. Human umbilical cord blood (hUCB) hold great potential in that immunotyped hUCB units can be immediately obtained from public banks....

Abstract The inhibition of tumor angiogenesis is considered a promising strategy to impede cancer progression, as the vasculature associated with tumors plays crucial role in supplying blood and facilitating metastasis. Therefore, vitro replication vascularized essential for comprehending pathology discerning mechanisms governing cell proliferation, metastasis, response pharmaceutical interventions. In this study, we fabricated tumor-on-a-chip recapitulate pathological features solid tumors....

Abstract In cancer immunotherapy, targeting specific antigens with chimeric antigen receptors (CARs) has emerged as a potent tool for cell-based therapy. After being accessed by CARs, the selective anticancer lysis CAR-natural killer (NK) cells through ‘missing-self’ and ‘induced-self’ mechanisms can mitigate off-tumor toxicity, contrast to CAR-T cells. This specificity be advantageous in heterogeneous milieu of solid tumors. tumor microenvironment glioblastoma (GBM), pericytes not only...

Hematopoietic stem and progenitor cells (HSPCs) are responsible for the lifetime dynamics of hematopoiesis, as they well known their self-renewing ability multipotency to differentiate into all types blood cells, including both myeloid lymphoid lineages. However, due limited amount accessibility, there is a strong need search out alternative methods produce HSPCs. In this review, we suggest induced pluripotent (iPSCs) new viable source HSPC production because these have potential self-renew...

Abstract Graphene quantum dots (GQDs) have been found to promote the efficient differentiation of stem cells into a variety cell types. However, their bioactivity and signaling mechanisms in hepatic lineage not fully investigated. Here, we report that hepatoblast (HB) rate can be promoted by using Ca 2+ -rich coffee bean (CB)-derived GQDs, where incorporated calcium ions activate insulin-like growth factor 1 receptor (IGF1R)-protein kinase B (AKT). We also human pluripotent (hPSC)-derived...

Abstract Colorectal cancer is one of leading cause cancer-related deaths. Therefore, there have been various attempts to cure the by developing new efficient anti-cancer therapy in addition surgical resection and chemotherapy. In this study, we investigated effects fenbendazole, an anti-helminthic drug, both colon cells patient-derived tumor organoids. Notably, employed 3D organoid models because 2D-cultured cell lines were not able recapitulate physiology solid tumors. We first observed...

In article number 2000195, Noo Li Jeon and co-workers reconstruct a 3D human lymphatic vessel network in self-organizing manner within high-throughput plastic array culture platform. With the capability of culturing multiple types cells comprising tumor immune microenvironment, model shows potential for its application studying roles vessels cancer therapeutics.

Tumor angiogenesis is regarded as a promising target for limiting cancer progression because tumor-associated vasculature supplies blood and provides path metastasis. Thus, in vitro recapitulation of vascularized tumors critical to understand the pathology identify mechanisms by which tumor cells proliferate, metastasize, respond drugs. In this study, we microengineered spheroid (VTS) model reproduce pathological features solid tumors. We first generated cell-EC hybrid spheroids with...

This study attempted to explore a beauty salon operation plan by investigating hairdressers’ perception of hygiene in the COVID-19 pandemic and analyzing differences depending on work patterns. For this, questionnaire survey was performed against managers from across country. A total 311 copies were used for final analysis, collected data analyzed, using SPSS WIN 21.0. To examine subjects’ general characteristics patterns, frequency analysis conducted. era analyze such cross-tabulation...