A5082543948- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Insect Pest Control Strategies
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Insect Utilization and Effects
- Bee Products Chemical Analysis
- Neurobiology and Insect Physiology Research
- Nematode management and characterization studies
- Mosquito-borne diseases and control
- Environmental Toxicology and Ecotoxicology
- Insects and Parasite Interactions
- Glycosylation and Glycoproteins Research
- Nutrition, Genetics, and Disease
- Carbohydrate Chemistry and Synthesis
- Viral Infections and Vectors
- Healthcare and Venom Research
- Marine and environmental studies
- Geological Formations and Processes Exploration
- Vector-borne infectious diseases
- Health and Medical Studies
- Insect-Plant Interactions and Control
Masaryk University
2016-2025
Glenfield Hospital
2014
Stockholm University
2010
Clotting systems are required in almost all animals to prevent loss of body fluids after injury. Here, we show that despite the risks associated with its systemic activation, clotting is a hitherto little appreciated branch immune system. We compared human blood and insect hemolymph study best-conserved component systems, namely Drosophila enzyme transglutaminase vertebrate homologue Factor XIIIa. Using labelled artificial substrates observe activity from both accumulates on microbial...
<i>Heterorhabditis bacteriophora</i> is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of species <i>Photorhabdus luminescens</i>. We performed a genome-wide transcriptional analysis <i>Drosophila</i> response to EPN infection at time point nematodes reached either via cuticle or gut and had started multiply. Many most strongly induced genes have been implicated in immune...
It has been known for many years that in temperate climates the European honey bee, Apis mellifera, exists form of two distinct populations within year, short-living summer bees and long-living winter bees. However, there is only limited knowledge about basic biochemical markers as yet. Nevertheless, distinction between these kinds becoming increasingly important it can help beekeepers to estimate proportion hives therefore part predict success overwintering. To identify generations, we...
We show that hemolymph clotting protects Drosophila melanogaster against infections with an entomopathogenic nematode and its symbiotic bacterium. also provide biochemical genetic evidence for involvement of eicosanoids in the same infection model. Taken together, our results confirm conserved nature immune function clot formation.
The extensive annual loss of honey bees (Apis mellifera L.) represents a global problem affecting agriculture and biodiversity. parasitic mite Varroa destructor, associated with viral co-infections, plays key role in this loss. Despite years intensive research, the complex mechanisms – bee interaction are still not fully defined. Therefore, study employed unique combination transcriptomic, proteomic, metabolomic, functional analyses to reveal new details about effect mites naturally factors,...
Entomopathogenic nematodes (EPNs) are efficient insect parasites, that known for their mutualistic relationship with entomopathogenic bacteria and use in biocontrol. EPNs produce bioactive molecules referred to as excreted/secreted products (ESPs), which have come the forefront recent years because of role process host invasion modulation its immune response. In present study, we confirmed production ESPs EPN Heterorhabditis bacteriophora, investigated phenoloxidase cascade, one key...
We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement activity in insect haemolymph.This method is the production signal depending viability bacterial cells.We observed significant rapid dose-dependent decrease using both species, Bombyx mori or Galleria mellonella haemolymph, was confirmed by determined plating.The humoral origin whole haemolymph for...
Photorhabdus asymbiotica is one of the three recognized species genus, which consists gram-negative bioluminescent bacteria belonging to family Morganellaceae. These live in a symbiotic relationship with nematodes from genus Heterorhabditis, together forming complex that highly pathogenic for insects. Unlike other species, are strictly entomopathogenic, P. unique its ability act as an emerging human pathogen. Analysis genome identified novel fucose-binding lectin designated PHL strong...
The infective juveniles (IJs) of entomopathogenic nematodes (EPNs) seek out host insects and release their symbiotic bacteria into body cavity causing septicaemia, which eventually leads to death. interaction between EPNs hosts are only partially understood, in particular the immune responses appears involve pathways other than phagocytosis canonical transcriptional induction pathways. These genetically tractable include for example clotting factors lipid mediators. aim this study was...
In temperate climates, honey bee workers of the species Apis mellifera have different lifespans depending on seasonal phenotype: summer bees (short lifespan) and winter (long lifespan). Many studies revealed biochemical parameters involved in lifespan differentiation bees. However, comprehensive information regarding metabolic changes occurring their bodies between two is limited. This study used proton nuclear magnetic resonance (1H NMR) spectroscopy to analyze differences same age. The...
Refl ex bleeding is one of the many anti-predation behavioural tactics used by insects.This behaviour recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples haemolymph physiological experiments.However, there no good evidence that refl blood have same characteristics.In this study, we compared three basic characteristics collected from body cavity, species ladybirds: Harmonia axyridis, Coccinella septempunctata...
The honey bee, Apis mellifera L., is one of the main pollinators worldwide. In a temperate climate, seasonality affects life span, behavior, physiology, and immunity bees. consequence, it impacts their interaction with pathogens parasites. this study, we used Bayesian statistics modeling to examine immune response dynamics summer winter bee workers after injection heat-killed bacteria Serratia marcescens , an opportunistic pathogen. We investigated humoral cellular at transcriptional...
Intensive agricultural practices impact the health and nutrition of pollinators like honey bees (
Abstract The harlequin ladybird is considered to be one of the most successful invasive insect species. Among other traits, its success caused by a powerful immune system. In present study, we investigate ontogenetic profile protein concentration, concentration circulating haemocytes and constitutive antimicrobial activity against Escherichia coli in Harmonia axyridis haemolymph during late larval development early adult life. Protein increases first 32 days life from 45 100 mg per mL...
In the temperate climates of central Europe and North America, two distinct honeybee (Apis mellifera) populations are found in colonies: short-living summer bees emerge spring survive until summer, whereas long-living winter late August overwinter. Besides difference their life spans, each these fulfils a different role colonies individual have physiological immunological adaptations depending on roles. For instance, worker higher vitellogenin levels larger reserves nutrients fat body than...
Abstract Overwintering is a challenging period in the life of temperate insects. A limited energy budget characteristic this can result reduced investment immune system. Here, we investigated selected physiological and immunological parameters laboratory‐reared field‐collected harlequin ladybirds ( Harmonia axyridis ). For beetles, focused on effects winter temperature regime (cold, average, or warm winter) total haemocyte concentration aiming to investigate potential ongoing climate change...
O-methylation is an unusual sugar modification with a function that not fully understood. Given its occurrence and recognition by lectins involved in the immune response, methylated sugars were proposed to represent conserved pathogen-associated molecular pattern. We describe interaction of O-methylated saccharides two β-propeller lectins, newly described PLL2 from entomopathogenic bacterium Photorhabdus laumondii, homologue PHL related human pathogen asymbiotica. The crystal structures...
Reactive oxygen species (ROS) are generated as products of normal cellular metabolic activities; however, the use pesticides to control leafcutter ants leads unbalanced ROS production. We evaluated effects two insecticides (fipronil, sulfluramid) and metallic insecticide complex (magnesium [Mg(hesp)2(phen)] (1)) on superoxide dismutase (SOD), glutathione (GSH) overall antioxidant capacity using different methodologies: total radical-trapping potential (TRAP) radical absorbance (ORAC). Media...