A5088344698- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Neuroblastoma Research and Treatments
- Developmental Biology and Gene Regulation
- Cancer, Hypoxia, and Metabolism
- Studies on Chitinases and Chitosanases
- Mosquito-borne diseases and control
- Physiological and biochemical adaptations
- Insect Utilization and Effects
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- Plant Molecular Biology Research
- Insect Pest Control Strategies
- Parasite Biology and Host Interactions
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Neurogenesis and neuroplasticity mechanisms
- Antimicrobial Peptides and Activities
- CRISPR and Genetic Engineering
- Insects and Parasite Interactions
- Cancer therapeutics and mechanisms
- Insect and Arachnid Ecology and Behavior
University of Gothenburg
2021-2023
Stockholm University
2013-2023
Science for Life Laboratory
2022-2023
Linköping University
2019-2020
<i>Heterorhabditis bacteriophora</i> is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of species <i>Photorhabdus luminescens</i>. We performed a genome-wide transcriptional analysis <i>Drosophila</i> response to EPN infection at time point nematodes reached either via cuticle or gut and had started multiply. Many most strongly induced genes have been implicated in immune...
High-risk neuroblastoma (NB) is responsible for a disproportionate number of childhood deaths due to cancer.One indicator highrisk NB amplification the neural MYC (MYCN) oncogene, which currently therapeutically intractable.Identification anaplastic lymphoma kinase (ALK) as an oncogene raised possibility using ALK tyrosine inhibitors (TKIs) in treatment patients with activating mutations.8-10% primary are ALK-positive, figure that increases relapsed population.ALK activated by ALKAL2 ligand...
Chitinase-like proteins (CLPs) of the 18 glycosyl hydrolase family retain structural similarity to chitinases but lack enzymatic activity. Although CLPs are upregulated in several human disorders that affect regenerative and inflammatory processes, very little is known about their normal physiological function. We show an insect CLP (<i>Drosophila</i> imaginal disc growth factor 3, IDGF3) plays immune-protective role during entomopathogenic nematode (EPN) infections. During these...
High-risk neuroblastoma (NB) often involves MYCN amplification as well mutations in ALK. Currently, high-risk NB presents significant clinical challenges, and additional therapeutic options are needed. Oncogenes like ALK result increased replication stress cancer cells, offering therapeutically exploitable options. We have pursued phosphoproteomic analyses highlighting ATR activity ALK-driven identifying the BAY1895344 inhibitor a potent of cell growth proliferation. Using RNA-Seq,...
Apart from their role in cellular immunity via phagocytosis and encapsulation, Drosophila hemocytes release soluble factors such as antimicrobial peptides, cytokines to induce humoral responses. In addition, they participate coagulation wounding, development. To assess during infection with entomopathogenic nematodes, we depleted plasmatocytes crystal cells, the two classes of present naïve larvae by expressing proapoptotic proteins order produce hemocyte-free (Hml-apo, originally called...
In most insects, hemolymph coagulation, which is analogous to mammalian blood clotting, involves close collaboration between humoral and cellular components. To gain insights into the secretion of clotting factors, we created tagged versions three different factors. Our focus was on factors are released in a non-classical manner characterize them comparison protein that classically released, namely Glutactin (Glt). Transglutaminase-A (Tg) Prophenoloxidase 2 (PPO2), both lack signal peptide...
In the developing Drosophila central nervous system (CNS), neural progenitor (neuroblast [NB]) selection is gated by lateral inhibition, controlled Notch signaling and proneural genes. However, mutants still generate many NBs, indicating existence of additional Moreover, recent studies reveal involvement key epithelial-mesenchymal transition (EMT) genes in NB selection, but regulatory interplay between EMT machinery unclear. We find that SoxNeuro (SoxB family) worniu (Snail are integrated...
Many leukemia patients suffer from dysregulation of their immune system, making them more susceptible to infections and leading general weakening (cachexia). Both adaptive innate immunity are affected. The fruit fly Drosophila melanogaster has an including cells the myeloid lineage (hemocytes). To study physiology during leukemia, we established three models by driving expression a dominant-active version Ras oncogene (RasV12 ) alone or combined with knockdowns tumor suppressors in...
Insect hemolymph coagulation: Kinetics of classically and non-classically secreted clotting factors Schmid et al., 2019. The linked article demonstrates the localization two secretory proteins in Drosophila melanogaster, Prophenoloxidase (PPO2) Transglutaminase-A (Tg) hemocytes as well clot with different tissue-specific drivers. Here we provide further data for usefulness GFP-tagged version crosslinking enzymes that are involved hardening. morphology crystal cells is described using PPO2...
One of the key factors that determine interaction between hosts and their parasites is frequency interactions, which depends on locomotory behavior both parts. To address host we used natural infections involving insect pathogenic nematodes Drosophila melanogaster larvae as hosts. Using a modified version recently described method (FIMTrack) to assess several parameters in larger sets animals, initially detected specific differences larval food searching when comparing strains. These were...
ABSTRACT Activating anaplastic lymphoma kinase (ALK) receptor tyrosine (RTK) mutations occur in pediatric neuroblastoma and are associated with poor prognosis. To study ALK-activating a genetically controllable system, we employed CRIPSR/Cas9, incorporating orthologs of the human oncogenic ALKF1174L ALKY1278S Drosophila Alk locus. AlkF1251L AlkY1355S mutant exhibited enhanced signaling phenotypes, but unexpectedly depended on Jelly belly (Jeb) ligand for activation. Both larval brains...
The acquisition of distinct branch sizes and shapes is a central aspect in tubular organ morphogenesis function. In the Drosophila airway tree, interplay apical extracellular matrix (ECM) components with underlying membrane cytoskeleton controls tube elongation, but link between ECM composition size regulation elusive. Here, we characterized Emp (epithelial protein), CD36 homolog belonging to scavenger receptor class B protein family. emp mutant embryos fail internalize luminal chitin...
ABSTRACT Development of the Drosophila visceral muscle depends on Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) signaling, which specifies founder cells (FCs) in circular mesoderm (VM). Although Alk activation by its ligand Jelly Belly (Jeb) is well characterized, few target molecules have been identified. Here, we used targeted DamID (TaDa) to identify targets embryos overexpressing Jeb versus with abrogated activity, revealing differentially expressed genes, including...
During cell cycle progression, the activity of CycE-Cdk2 complex gates S-phase entry. is inhibited by CDK inhibitors (CKIs) Cip/Kip family, which include human p21Cip1 and Drosophila Dacapo (Dap) proteins. Both CycE family proteins are under elaborate control via protein degradation, mediated Cullin-RING ligase (CRL) ubiquitin complexes. The CRL SCFFbxw7/Ago targets phosphorylated CycE, whereas Dap targeted CRL4Cdt2 complex, binding to PIP degron. role CRL-mediated degradation during CNS...
Abstract The wap gene encodes a single whey acidic protein (WAP) domain-containing peptide from Chinese white shrimp ( Fenneropenaeus chinensis ), which shows broad-spectrum antimicrobial activities and proteinase inhibitory in vitro . To explore the medical applications of WAP peptide, transgenic Drosophila melanogaster was constructed. In -expressing flies, high expression levels (>100 times) were achieved, contrast to those control by qRT-PCR analysis. associated with increased...