A5024364453- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Neurobiology and Insect Physiology Research
- Aquaculture disease management and microbiota
- Insect and Arachnid Ecology and Behavior
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Mosquito-borne diseases and control
Stockholm University
2017-2019
Umeå University
2010-2016
University of Würzburg
2007
Abstract Because NF-κB signaling pathways are highly conserved in evolution, the fruit fly Drosophila melanogaster provides a good model to study these cascades. We carried out an RNA interference (RNAi)-based genome-wide vitro reporter assay screen for components of pathways. analyzed 16,025 dsRNA-treatments and identified 10 novel regulators. Of these, nine affect primarily Toll pathway. G protein-coupled receptor kinase (Gprk)2, CG15737/Toll pathway activation mediating protein, u-shaped...
The Toll signaling pathway, first discovered in Drosophila, has a well-established role immune responses insects as well mammals. In the Toll-dependent induction of antimicrobial peptide production been intensely studied model for innate general. Besides this humoral response, is also known to activate blood cells reaction that similar cellular response parasite infections, but mechanisms are poorly understood. Here we have detail, and found several different tissues can defense, does not...
In most insects, hemolymph coagulation, which is analogous to mammalian blood clotting, involves close collaboration between humoral and cellular components. To gain insights into the secretion of clotting factors, we created tagged versions three different factors. Our focus was on factors are released in a non-classical manner characterize them comparison protein that classically released, namely Glutactin (Glt). Transglutaminase-A (Tg) Prophenoloxidase 2 (PPO2), both lack signal peptide...
Insect hemolymph coagulation: Kinetics of classically and non-classically secreted clotting factors Schmid et al., 2019. The linked article demonstrates the localization two secretory proteins in Drosophila melanogaster, Prophenoloxidase (PPO2) Transglutaminase-A (Tg) hemocytes as well clot with different tissue-specific drivers. Here we provide further data for usefulness GFP-tagged version crosslinking enzymes that are involved hardening. morphology crystal cells is described using PPO2...
To understand how Toll signaling controls the activation of a cellular immune response in Drosophila blood cells (hemocytes), we carried out genetic modifier screen, looking for deletions that suppress or enhance mobilization sessile hemocytes by gain-of-function mutation Toll10b (Tl10b). Here describe results from chromosome arm 3R, where five regions strongly suppressed this phenotype. We identified specific genes deficient 1 (ird1), headcase (hdc) and possibly Rab23 as suppressors,...