A5006853324- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Nematode management and characterization studies
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Insect behavior and control techniques
- Health Education and Validation
- Genomics and Phylogenetic Studies
- Insect Utilization and Effects
- Forest Insect Ecology and Management
- Insect-Plant Interactions and Control
- Invertebrate Taxonomy and Ecology
- Parasite Biology and Host Interactions
- Enzyme Production and Characterization
- Healthcare during COVID-19 Pandemic
- Biofuel production and bioconversion
- Silk-based biomaterials and applications
- Invertebrate Immune Response Mechanisms
- Health, Nursing, Elderly Care
- Bee Products Chemical Analysis
- Mitochondrial Function and Pathology
- Technology in Education and Healthcare
- Identification and Quantification in Food
- Advertising and Communication Studies
Universidade dos Açores
2013-2025
Secretaria Regional do Ambiente e Recursos Naturais
2024
University of Girona
2023
National Education and Research Network
2010-2016
Universidade de Brasília
2014
Institute for Biotechnology and Bioengineering
2013
Hospital de Santo Espirito de Angra do Heroísmo
2001
ABSTRACT Xenorhabdus nematophila , a bacterium pathogenic for insects associated with the nematode Steinernema carpocapsae releases high quantities of proteases, which may participate in virulence against insects. Zymogram assays and cross-reactions antibodies suggested that two distinct proteases were present. The major one, protease II, was purified shown to have molecular mass 60 kDa an estimated isoelectric point 8.5. Protease II digested chromogenic substrate N...
A trypsin-like serine protease was purified by gel filtration and anion-exchange chromatography from the excretory-secretory products of parasitic phase Steinernema carpocapsae. The exhibited a molecular mass about 29 kDa SDS-PAGE displayed pI 6.3. This high activity with trypsin-specific substrate N-Ben-Phe-Val-Arg-p-nitroanilide highly sensitive to aprotinin benzamidine. trypsin digested chromogenic K(m), V(max) k(cat) values 594.2 mum, 0.496 mum/min 22.8/s, respectively. optimal pH...
Steinernemacarpocapsae is a nematode pathogenic in wide variety of insect species. The great pathogenicity this has been ascribed to its ability overcome the host immune response; however, little known about mechanisms involved process. analysis an expressed sequence tags (EST) library during infective phase was performed and highly abundant contig homologous serine protease inhibitors identified. In work, we show that part 641-bp cDNA encodes BPTI-Kunitz family inhibitor (Sc-KU-4), which...
Steinernema carpocapsae is an entomopathogenic nematode widely used for the control of insect pests due to its virulence, which mainly attributed ability parasitic stage has overcome defences. To identify mechanisms underlying such a characteristic, we studied novel serpin-like inhibitor (sc-srp-6) that was detected in transcriptome analysis. Recombinant Sc-SRP-6 produced Escherichia coli had native fold serpins belonging α-1-peptidase family and exhibited inhibitory activity against trypsin...
ABSTRACT Photorhabdus sp. strain Az29 is symbiotic with an Azorean nematode of the genus Heterorhabditis in a complex that highly virulent to insects even at low temperatures. The virulence bacteria mainly attributed toxins and bacterial enzymes secreted during parasitism. secrete proteases growth, peak end exponential growth phase. Protease secretion was higher cultures growing lower At 10°C activity highest remained constant for over 7 days, whereas 23 28°C it showed steady decrease. Two...
Steinernema carpocapsae is an insect parasitic nematode used in biological control, which infects insects penetrating by mouth and anus invading the hemocoelium through midgut wall. Invasion has been described as a key factor virulence suggested to be mediated proteases. A serine protease cDNA from stage was sequenced (sc-sp-1); recombinant protein produced Escherichia coli system, native purified secreted products. Both proteins were confirmed mass spectrometry encoded sc-sp-1 gene. Sc-SP-1...
Abstract The entomopathogenic nematode Steinernema carpocapsae has been widely used for the biological control of insect pests. It shares a symbiotic relationship with bacterium Xenorhabdus nematophila , and is emerging as genetic model to study symbiosis pathogenesis. We obtained high-quality draft nematode’s genome comprising 84,613,633 bp in 347 scaffolds, an N50 1.24 Mb. To improve annotation, we sequenced both short long RNA conducted shotgun proteomic analyses. S. orthologous genes...
The mechanisms of infection and pathogenicity Steinernematidae Heterorhabditidae in insect hosts are discussed as factors influencing the host specificity these nematodes. invasion evasion defences important steps pathogenic process. ability nematode to penetrate into haemocoel, achieved by release proteolytic enzymes, is one specific factor. Another factor nematode-insect relationship evade through failure be recognized and/or destruction antibacterial factors. Toxins extracellular enzymes...
Ancient DNA (aDNA) analysis can be a useful tool in bacterial disease diagnosis human remains. However, while the recovery of Mycobacterium spp. has been widely successful, several authors report unsuccessful results regarding ancient treponemal DNA, casting doubts on usefulness this technique for syphilis. Here, we present from an four newborn specimens recovered crypt "La Ermita de la Soledad" (XVI–XVII centuries), located province Huelva southwest Spain. We extracted and analyzed aDNA...
In the present study, effect of bovine oocyte quality related to ultrastructural characteristics zona pellucida (ZP), polyspermic penetration and embryo developmental competence was evaluated. Cumulus-oocyte complexes were punctioned from 453 ovaries, classified as 1, 2, 3 4 according their morphological aspect, matured for 24 h then divided into two groups. group A, oocytes fixed in 2.5% glutaraldehyde 0.1 m sodium cacodylate examined under a scanning electron microscope. Photomicrographs...
Entomopathogenic nematodes (EPNs) are efficient insect parasites, that known for their mutualistic relationship with entomopathogenic bacteria and use in biocontrol. EPNs produce bioactive molecules referred to as excreted/secreted products (ESPs), which have come the forefront recent years because of role process host invasion modulation its immune response. In present study, we confirmed production ESPs EPN Heterorhabditis bacteriophora, investigated phenoloxidase cascade, one key...
Abstract Steinernema carpocapsae is an entomopathogenic nematode (EPN) that rapidly infects and kills a wide range of insect hosts has been linked to host immunosuppression during the initial stages infection. The lethal nature S. infections previously credited its symbiotic bacteria; however, it become evident nematodes are able effectively kill their independently through excretion/secretion products (ESPs). Here we examined how adult Drosophila melanogaster immune system modulated in...