A5004998036- Plant-Microbe Interactions and Immunity
- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Streptococcal Infections and Treatments
- Fungal Biology and Applications
- Enzyme Production and Characterization
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Studies on Chitinases and Chitosanases
- Cancer Research and Treatments
- Pediatric Urology and Nephrology Studies
- Bladder and Urothelial Cancer Treatments
- Neonatal and Maternal Infections
- Insect symbiosis and bacterial influences
- Plant tissue culture and regeneration
- Veterinary Medicine and Surgery
- Biofuel production and bioconversion
- Carbohydrate Chemistry and Synthesis
- Pneumonia and Respiratory Infections
- Plant Pathogenic Bacteria Studies
- Mycorrhizal Fungi and Plant Interactions
- Essential Oils and Antimicrobial Activity
- Radiopharmaceutical Chemistry and Applications
Madurai Kamaraj University
2019-2023
Universidade Nova de Lisboa
2015-2019
Saveetha University
2017
Rede de Química e Tecnologia
2017
University of Lisbon
2015
University of Madras
1989-2014
Universidade dos Açores
2009-2014
Institute for Biotechnology and Bioengineering
2013
Tamil Nadu Agricultural University
2000
Madras Medical College
1996
A total of 39 chitinolytic bacteria were isolated from 77 rhizosphere soil samples collected different crop fields in Tamil Nadu state, India. Among them, a strain designated as MML2270, which produced highest activity primary and secondary screening colloidal chitin agar was selected later identified Bacillus laterosporous. The production chitinase by B. laterosporous optimized using growth media, substrate concentrations, pH, temperature incubation period. maximum observed yeast nitrogen...
A trypsin-like serine protease was purified by gel filtration and anion-exchange chromatography from the excretory-secretory products of parasitic phase Steinernema carpocapsae. The exhibited a molecular mass about 29 kDa SDS-PAGE displayed pI 6.3. This high activity with trypsin-specific substrate N-Ben-Phe-Val-Arg-p-nitroanilide highly sensitive to aprotinin benzamidine. trypsin digested chromogenic K(m), V(max) k(cat) values 594.2 mum, 0.496 mum/min 22.8/s, respectively. optimal pH...
The most important and economically cultivated cotton plant was selected to test the growth promotion by Trichoderma viride and Pseudomonas fluroescens with without pathogens, Rhizoctonia solani and Macrophomina phaseolina. Of these, T. viride was found be more effective than P. fluroescens on shoot root length elongation. Seed germination percentage, length, fresh weight, dry weight vigour index were significantly increased by T. virideand P. fluroescens. T. viride inoculated plants 2.7 2.4...
Steinernema carpocapsae is an entomopathogenic nematode widely used for the control of insect pests due to its virulence, which mainly attributed ability parasitic stage has overcome defences. To identify mechanisms underlying such a characteristic, we studied novel serpin-like inhibitor (sc-srp-6) that was detected in transcriptome analysis. Recombinant Sc-SRP-6 produced Escherichia coli had native fold serpins belonging α-1-peptidase family and exhibited inhibitory activity against trypsin...
We have shown, the outcome of antifungal activity phenazine derivatives which is produced by fluorescent pseudomonads (FPs) for control sheath blight rice. A total 50 were isolated from rice rhizosphere. Off which, 36 FPs exhibited antagonistic against Rhizoctonia solani, Macrophomina phaseolina, Fusarium oxysporum, Alternaria alternata and Sclerotium rolfsii up to 70–80% compared dual culture method. BOX-PCR analyses isolates indicated that two phylogenetic group, where group I consisted 28...
A protocol for isolating and regenerating protoplasts from Trichothecium roseum has been described. Protoplasts T. were isolated using (i) a lytic enzyme combination composed of Novozym 234, chitinase, cellulase, pectinase at 5-mg/mL concentration (ii) 0.6 M KCl as an osmotic stabilizer. maximum number 28 x 10(4) protoplasts/mL obtained pH 5.5. Experiments on the regeneration reversion revealed (60.8%) in complete medium (potato dextrose--yeast extract agar) amended with KCl. The regenerated...
Streptococcus dysgalactiae subsp. (SDSD), a Lancefield group C streptococci (GCS), is frequent cause of bovine mastitis. This highly prevalent disease the costliest in dairy industry. Adherence and biofilm production are important factors streptoccocal pathogenesis. We have previously described adhesion internalization SDSD isolates human cells now we describe capability this bacterium. In work integrated microbiology, imaging computational methods to evaluate isolates; assess presence...
Antimicrobial materials based on hydroxyapatite nanopowders are functionally attractive in a wide variety of medical applications. The Cu 2+ and Zn incorporated HAp were synthesized by simple Sol-Gel method. structural study, optical study biological activity the prepared characterized X-ray diffraction, UV-Vis absorption spectroscopy disc diffusion method respectively. XRD results demonstrated that presence copper zinc doped size crystalline was found for pure HAp, 10%, 25% 50% / metal ions...
The efficacy of a rhizobacterium Streptomyces aurantiogriseus VSMGT1014 for the production bioactive metabolites with antifungal properties was evaluated under in vitro conditions. by S. International Project‐2 (ISP‐2) broth, supplemented glucose and ammonium acetate found to be most suitable carbon nitrogen sources maximum against rice pathogen, Rhizoctonia solani . zone inhibition range from 23.5 28.5 mm 10.3 18.3 media, respectively. culture filtrate S at pH 7.5, 37 °C 120 rpm 6 days...
Indian soils are rich in microbial diversity, especially fluorescent pseudomonads (FPs) have drawn much attention worldwide because of their plant growth promotion ability by the production promoting substance like Indole acetic acid (IAA). In this context, present study explored for optimization and characterization IAA our isolate Pseudomonas sp VSMKU 4050. The maximum was observed King’s B broth (KBB) supplemented with 0.7% L – tryptophan. KBB medium recognized as best production, while...
A standard method for isolation, fusion and regeneration of protoplasts from Trichoderma harzianum viride was developed. The T. were isolated using Novozym 234 as lytic enzyme potassium chloride osmotic stabilizer. maximum number (9.37 × 105/ml) obtained 16 h old mycelium 11.1 105/ml 14 at pH 5.5, 28oC 3 h. interspecific intraspecific frequency determined 40% polyethylene glycol (PEG) fusogen. intrafusants selected based on their growth, sporulation, pigmentation chitin cellulose amended...
Abstract A novel and simple wet chemical hydrothermal synthesis method was employed in the preparation of zinc oxide (ZnO) nanoparticles using neem (N), pepper (P) turmeric (T) extracts as solvent media. The structural optical properties well antibacterial anticancer all samples (ZnO, N/ZnO, P/ZnO, T/ZnO NPT/ZnO) were characterized analyzed. Solvent media found to have an effect on both size morphology nanoparticles, which turn effected their cytotoxic properties. colony forming unit (CFU)...