A5033592957- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Endometriosis Research and Treatment
- Ovarian function and disorders
- Reproductive tract infections research
- Gut microbiota and health
- Vitamin D Research Studies
- Pelvic floor disorders treatments
- Sperm and Testicular Function
- Silk-based biomaterials and applications
- Cytomegalovirus and herpesvirus research
- Adhesion, Friction, and Surface Interactions
- Ovarian cancer diagnosis and treatment
- CRISPR and Genetic Engineering
- Assisted Reproductive Technology and Twin Pregnancy
- Genetic and phenotypic traits in livestock
- Electrospun Nanofibers in Biomedical Applications
- Urological Disorders and Treatments
- Animal Genetics and Reproduction
- Genomics and Chromatin Dynamics
- Spaceflight effects on biology
- Marine Biology and Environmental Chemistry
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Bone Tissue Engineering Materials
Instituto de Investigación Biosanitaria de Granada
2023-2025
Universidad de Granada
2023-2024
Universidad de Murcia
2018-2024
Instituto Murciano de Investigación Biosanitaria
2021-2023
Universidade dos Açores
2016
Barco (Belgium)
2015
University of Minho
2015
In recent years, high-throughput sequencing technologies have revolutionised reproductive microbiome research. Our understanding of the endometrial is primarily based on DNA-based 16S rRNA gene profiling, but DNA detection does not imply alive microbe presence. While this method cost-effective and widely used, it has notable limitations, including underestimation microbial diversity, abundance, functionality, as well limited species-level resolution. Meta-transcriptomic analysis, an...
Introduction Several metabolite classes have been identified in human endometrium, including lipids, nucleotides, amino acids, organic and sugars. The first studies suggest the importance of metabolites endometrial functions, as imbalance uterine has associated with low implantation rate endometriosis. Nevertheless, most put emphasis on specific classes, we lack knowledge whole metabolome composition uterus. Further, a healthy dietary pattern shown to potentially protect against different...
Introduction The female reproductive tract harbours unique microbial communities (known as microbiota) which have been associated with functions in health and disease. While endometrial microbiome studies shown that the uterus possesses higher bacterial diversity richness compared to vagina, knowledge regarding composition of Fallopian tubes (FT) is lacking, especially fertile women without any underlying conditions. Methods To address this gap, our study included 19 patients who underwent...
Abstract Background Endometriosis, defined as the presence of endometrial-like tissue outside uterus, is one most prevalent gynecological disorders. Although different theories have been proposed, its pathogenesis not clear. Novel studies indicate that gut microbiome may be involved in etiology endometriosis; nevertheless, connection between microbes, their dysbiosis, and development endometriosis understudied. This case–control study analyzed women with without to identify microbial targets...
Studies of knockout (KO) mice with defects in the endolysosomal two-pore channels (TPCs) have shown TPCs to be involved pathophysiological processes, including heart and muscle function, metabolism, immunity, cancer, viral infection. With objective studying TPC2's roles for first time a large, more humanlike animal model, TPC2 KO pigs were produced using CRISPR-Cas9. A major problem CRISPR-Cas9 edit embryos is mosaicism; thus, we studied effect microinjection timing on mosaicism. Mosaicism...
Hierarchical structures, constituted by polymeric nano and microfibers, have been considered promising scaffolds for tissue engineering strategies, mainly because they mimic, in some way, the complexity nanoscale detail observed real organs. The chondrogenic potential of these has previously demonstrated, but their osteogenic is not yet corroborated. In order to assess if a hierarchical structure, with details incorporated, an improved scaffold bone regeneration, we evaluate cell adhesion,...
The improvement of the embryo culture media is high relevance due to its influence on successful implantation rates, pregnancy, neonatal outcomes, and potential effects in adult life. ideal conditions for development are those naturally occurring female reproductive tract, i.e., oviductal uterine fluids. To shed light differences between chemical natural media, we performed first comparative study low abundance proteins plasma, uterine, fluid collected, simultaneously, from healthy fertile...
Abstract The addition of reproductive fluids (RF) to the culture media has shown benefits in different embryonic traits but its long-term effects on offspring phenotype are still unknown. We aimed describe such pigs. Blood samples and growth parameters were collected from piglets derived vitro-produced embryos (IVP) with or without RF added versus those artificially inseminated (AI), day 0 month 6 life. An oral glucose tolerance test was performed 45 show here first comparative data animals...
The developing embryo is a paradigmatic model to study molecular mechanisms of time control in Biology. Hox genes are key players the specification tissue identity during development and their expression under strict temporal regulation. However, underlying timely activation early remain unknown. This hindered by lack rigorous framework sequential within single cluster. Herein, thorough characterization HoxB cluster gene was performed over space chick embryo. Clear collinearity observed....
Abstract Background Endometriosis, defined as the presence of endometrial-like tissue outside uterus, is one most prevalent gynecological disorders. Although different theories have been proposed, its pathogenesis not clear. Novel studies indicate that gut microbiome may be involved in etiology endometriosis, nevertheless, connection between microbes, their dysbiosis and development endometriosis understudied. This case-control study analyzed women with without to identify microbial targets...
Nitric oxide, a key regulatory molecule in the follicular fluid, has been suggested as possible biomarker to predict ovarian response stimulated cycles and potential of retrieved oocytes for developing high-quality embryos. Nevertheless, consensus on whether or not nitric oxide can help this context reached. We simultaneously measured oxidation products nitrite, nitrate, via high-performance liquid chromatography (HPLC)-UV fluid samples from 72 oocyte donors. found no associations total...
Abstract The egg is a spherical cell encapsulated by the zona pellucida (ZP) which forms filamentous matrix composed of several glycoproteins that mediate gamete recognition at fertilization. Studies on molecular mechanisms sperm-egg binding are limited in many mammalian species scarcity eggs, ethical concerns harvesting and high cost producing genetically modified animals. To address these limitations, we have reproduced three-dimensional (3D) model mimicking oocyte’s shape, means magnetic...
Assisted reproductive technologies play a major role in the cattle industry. An increase use of vitro -derived embryos is currently being seen around globe. But efficiency and quality are substandard when compared to vivo production. Different protocols have been designed overcome this issue, one those fluids as supplementation embryo culture media. In study, calves produced with added their production protocol were followed for first year life pairwise control, by artificial insemination...
Culture media supplemented with reproductive fluids (RF) have been used in livestock species, improving the efficiency and quality of vitro produced embryos. However, usefulness humans is still unknown. In this study, we collected human (HRFs) ex vivo (from 25 patients undergoing abdominal hysterectomy plus bilateral salpingectomy) 31 oocyte donors). Afterward, protocols to evaluate their osmolality, pH, total protein concentration, endotoxin level, sterility were optimized, establishing...
Abstract The female reproductive tract harbours its microbial communities (microbiota) and composition has been associated with functions in health disease. Endometrial microbiome (genomes of microbes) studies are agreement that uterus possesses higher bacterial diversity richness than the vagina (where Lactobacillus is dominating ) but knowledge Fallopian tubes (FT) microbiomal lacking, especially fertile women disease-free conditions. Our study included 24 confirmed fertility having benign...
Abstract Reproductive fluids have been recently proposed as the most adequate natural additive to complement chemically defined in vitro culture media different animal models, since they contain full range of molecules necessary for fertilization and development preimplantation embryos. However, reproductive potentially are problematic terms biosecurity reproducibility results, their availability is scarce. Therefore, we optimized protocols characterize physico-chemical functional properties...