A5102022601- MicroRNA in disease regulation
- RNA Research and Splicing
- RNA modifications and cancer
- Marine Biology and Environmental Chemistry
- Coral and Marine Ecosystems Studies
- Alzheimer's disease research and treatments
- RNA regulation and disease
- Silk-based biomaterials and applications
- Extracellular vesicles in disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Coastal and Marine Management
- RNA Interference and Gene Delivery
- International Maritime Law Issues
- Adhesion, Friction, and Surface Interactions
- Endoplasmic Reticulum Stress and Disease
- Chromatin Remodeling and Cancer
- Fish Biology and Ecology Studies
- Physical Education and Sports Studies
- High Altitude and Hypoxia
- Surface Modification and Superhydrophobicity
- Diamond and Carbon-based Materials Research
- Nuclear Structure and Function
- Immune cells in cancer
- Polymer Surface Interaction Studies
- Tourism, Volunteerism, and Development
University of Nottingham
2018-2024
University of Lisbon
2010-2018
Iscte – Instituto Universitário de Lisboa
2013
Mutations in SWI/SNF genes are amongst the most common across all human cancers, but efficient therapeutic approaches that exploit vulnerabilities caused by mutations currently lacking. Here, we show ATPases BRM/SMARCA2 and BRG1/SMARCA4 promote expression of p62/GTF2H1, a core subunit transcription factor IIH (TFIIH) complex. Inactivation either ATPase downregulates GTF2H1 therefore compromises TFIIH stability function nucleotide excision repair (NER). We also demonstrate cells with...
Transthyretin amyloidosis is a conformational pathology characterized by the extracellular formation of amyloid deposits and progressive impairment peripheral nervous system. Point mutations in this tetrameric plasma protein decrease its stability are linked to disease onset progression. Since non-mutated transthyretin also forms systemic senile some mutation bearers asymptomatic throughout their lives, non-genetic factors must be involved amyloidosis. We discovered, using differential...
Both the establishment of neuronal polarity and axonal growth are crucial steps in development nervous system. The local translation mRNAs axon provides precise regulation protein expression, is now known to participate development, pathfinding synaptic formation function. We have investigated role miR-26a early stage mouse primary cortical neuron development. show that micro-RNA-26a-5p (miR-26a) highly expressed cultures, regulates both growth. Using compartmentalised microfluidic we...
Programmed cell death 4 (PDCD4) protein is a tumor suppressor that inhibits translation through the mTOR-dependent initiation factor EIF4A, but its functional role and mRNA targets in neurons remain largely unknown. Our work identified PDCD4 highly expressed axons dendrites of CNS PNS neurons. Using loss- gain-of-function experiments cortical dorsal root ganglia primary neurons, we demonstrated capacity to negatively control axonal growth. To explore transcriptome translatome targets, used...
We report the total synthesis of seven Illicium -derived neolignans along with experimental evidence which unites homooligomers and apparent heterooligomers under a new biosynthesis proposal that features single common precursor.
Quantum diamond sensors containing Nitrogen Vacancy (NV) centers were integrated into the culture of spontaneously electrically active cultures live mouse primary cortical neurons. Two formats used enabling extracellular studies cells cultured directly on a planar plate and intracellular via endocytosis mediated integration nanoscale particles within cells. Over 14 day period functional neuronal networks formed as confirmed by endogenous calcium transients detected fluorescent imaging probe...
Protein misfolding disorders such as Alzheimer, Parkinson and transthyretin amyloidosis are characterized by the formation of protein amyloid deposits. Although nature location aggregated proteins varies between different diseases, they all share similar molecular pathways unfolding, aggregation deposition. Most effects these likely to occur at proteome level, a virtually unexplored reality. To investigate an expression on cellular proteome, we created yeast system using human (TTR) model...
Neurons have highlighted the needs for decentralized gene expression and specific RNA function in somato-dendritic axonal compartments, as well intercellular communication via extracellular vesicles (EVs). Despite advances miRNA biology, identity regulatory capacity of other small non-coding RNAs (sncRNAs) neuronal models local subdomains has been largely unexplored.We identified a highly complex differentially localized content sncRNAs axons EVs during early development cortical primary...
Sea urchins have specialized adhesive organs called tube feet, which mediate strong but reversible adhesion. Tube feet are composed by a disc, producing and de-adhesive secretions for substratum attachment, stem movement. After detachment the secreted remains bound to as footprint. Recently, label-free quantitative proteomic approach coupled with latest mass-spectrometry technology was used analyze differential proteome of Paracentrotus lividus organ, comparing protein expression levels in...
Transthyretin amyloidosis (ATTR) belongs to a class of disorders caused by protein misfolding and aggregation. ATTR is disabling disorder autosomal dominant trait, where transthyretin (TTR) forms amyloid deposits in different organs, causing dysfunction the peripheral nervous system. We previously discovered that fibrils from patients are glycated methylglyoxal. Even though no consensus has been reached about actual role methylglyoxal-derived advanced glycation end-products diseases,...
Extracellular vesicles (EVs) are secreted by all cells in the CNS, including neurons and astrocytes. EVs lipid membrane enclosed particles loaded with various bioactive cargoes reflecting dynamic activities of origin. In contrast to neurons, specific role released astrocytes is less well understood, partly due difficulty maintaining primary astrocyte cultures a quiescent state. The aim this study was establish human serum-free culture system that maintains state morphology, function, protein...
Los límites entre las especies del género Dasyprocta aún están en debate y la distribución de pacas también es incierta, especialmente Caatinga, donde D. prymnolopha nigriclunis existen hay discordancias sobre sí estos taxones deben ser sinonimizados o no. Esta nota tiene como objetivo reportar nuevos registros obtenidos mediante el monitoreo con cámaras trampa, lo que amplía su área distribución. El levantamiento se realizó Parque Nacional Catimbau, estado Pernambuco, junio 2023 a julio...
This paper presents three energy services that are being developed in the marketplace as part of European project BD4OPEM H2020. The based on optimization and machine learning techniques cover different distribution grid operation applications such congestion forecasting, flexibility planning for networks. work focuses combination execution strategy services. scheme is tested a real medium voltage located Spain.