A5086144872- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- RNA regulation and disease
- Reproductive Biology and Fertility
- Genetic and Kidney Cyst Diseases
- Neurogenesis and neuroplasticity mechanisms
- Pesticide and Herbicide Environmental Studies
- Genetics, Aging, and Longevity in Model Organisms
- Cellular transport and secretion
- Pesticide Exposure and Toxicity
- Stress Responses and Cortisol
- Adipose Tissue and Metabolism
- Renal and related cancers
- Animal Genetics and Reproduction
- Origins and Evolution of Life
- Diet, Metabolism, and Disease
- Healthcare and Venom Research
- Tryptophan and brain disorders
- Cholinesterase and Neurodegenerative Diseases
- Environmental Toxicology and Ecotoxicology
- MicroRNA in disease regulation
University of Nottingham
2013-2024
Queen's Medical Centre
2005-2024
Versus Arthritis
2024
Rathbone Hospital
2016
Google (United States)
2016
Medical Research Council
2007
Lack of physical activity and increased levels stress contribute to the development multiple mental disorders. An increasing number studies relate voluntary exercise with greater resilience psychological stress, a process that is highly regulated by hypothalamic-pituitary-adrenal (HPA) axis. However, molecular mechanisms underlying beneficial effects on are still poorly understood. Here we have studied impact long term housing conditions on: a) hippocampal expression glucocorticoid receptor...
Abstract The health of cloned animals generated by somatic-cell nuclear transfer (SCNT) has been concern since its inception; however, there are no detailed assessments late-onset, non-communicable diseases. Here we report that SCNT obvious detrimental long-term effects in a cohort 13 sheep. We perform musculoskeletal assessments, metabolic tests and blood pressure measurements aged (7–9 years old) sheep, including four derived from the cell line gave rise to Dolly. also radiological...
Cortical collapse factors affect microtubule (MT) dynamics at the plasma membrane. They play important roles in neurons, as suggested by inhibition of axon growth and regeneration through ARF activator Efa6 C. elegans, neurodevelopmental disorders linked to mammalian kinesin Kif21A. How cortical influence is little understood. Here we studied them, focussing on function Drosophila experimentally genetically amenable fly neurons. First, show that can inhibit MTs directly without interacting...
There is a need for mechanistic understanding of the lasting ill health reported in several studies workers exposed to organophosphorus (OP) pesticide. Although acute toxicity largely explicable by acetylcholinesterase inhibition and effects frank poisoning direct excitotoxicity or indirect consequences cholinergic syndrome, at lower levels exposure would not be predicted from these mechanisms. Similarly, reversible interactions with nicotinic muscarinic receptors adults predict continuing...
The birth of live animals following somatic cell nuclear transfer (SCNT) has demonstrated that oocytes can reprogram the genome differentiated cells. However, in all species frequency development healthy offspring is low; for example, sheep, approximately only 5% blastocysts transferred develop to term, and less than 3% adulthood. Such low efficiencies, coupled with occurrence developmental abnormalities, have been attributed incomplete or incorrect reprogramming. Cytoplasmic extracts from...
Both the establishment of neuronal polarity and axonal growth are crucial steps in development nervous system. The local translation mRNAs axon provides precise regulation protein expression, is now known to participate development, pathfinding synaptic formation function. We have investigated role miR-26a early stage mouse primary cortical neuron development. show that micro-RNA-26a-5p (miR-26a) highly expressed cultures, regulates both growth. Using compartmentalised microfluidic we...
Kinesins that influence the dynamics of microtubule growth and shrinkage require ability to distinguish between end lattice. The depolymerizing kinesin MCAK has been shown specifically recognize end. This is key action in regulating dynamics. We show α4-helix motor domain crucial recognition. Mutation residues K524, E525 R528, which are located C-terminal half α4-helix, disrupts these residues, conserved kinesin-13 family discriminate members this from translocating kinesins, impairs lattice
Our recent report that fructose supported the metabolism of some, but not all axons, in adult mouse optic nerve prompted us to investigate detail this tissue, a typical central white matter tract, as these data imply efficient nervous system (CNS). In artificial cerebrospinal fluid containing 10 mmol/L glucose or 20 fructose, stimulus-evoked compound action potential (CAP) recorded from consisted three stable peaks. Replacing with however, caused delayed loss 1st CAP peak (the 2nd and 3rd...
The reprogramming of somatic cells into a pluripotent/embryonic-like state holds great potential for regenerative medicine, bypassing ethical issues associated with embryonic stem (ESCs). Numerous methods, including cell nuclear transfer (SCNT), fusion to pluripotent cells, the use extracts, and expression transcription factors, have been used reprogram ES-like [termed induced (iPSCs)]. This study investigated early events in nuclei permeabilized murine incubated cytoplasmic extract prepared...
Neurons have highlighted the needs for decentralized gene expression and specific RNA function in somato-dendritic axonal compartments, as well intercellular communication via extracellular vesicles (EVs). Despite advances miRNA biology, identity regulatory capacity of other small non-coding RNAs (sncRNAs) neuronal models local subdomains has been largely unexplored.We identified a highly complex differentially localized content sncRNAs axons EVs during early development cortical primary...
The kinesin superfamily of microtubule associated motor proteins share a characteristic domain which both hydrolyses ATP and binds microtubules. Kinesins display differences across the in turnover interaction. These tailor specific kinesins to various functions such as cargo transport, sliding, depolymerization stabilization. To understand mechanism action it is important how chemical cycle coupled mechanical dissect cycle, one approach utilize fluorescently labeled nucleotides visualize...
The activity of a kinesin is largely determined by the approximately 350 residue motor domain, and this region alone sufficient to classify as member particular family. kinesin-13 family are group microtubule depolymerizing kinesins vital regulators length. Kinesin-13s critical spindle assembly chromosome segregation in both mitotic meiotic cell division play crucial roles cilium length control neuronal development. To better understand evolution depolymerization activity, we created...
Abstract Cortical collapse factors affect microtubule (MT) dynamics at the plasma membrane. They play important roles in neurons, as suggested by inhibition of axon growth and regeneration through Arf activator Efa6 C. elegans , neurodevelopmental disorders linked to mammalian kinesin Kif21A. How cortical influence is little understood. Here we studied them, focussing on function Drosophila experimentally genetically amenable fly neurons. First, show that can inhibit MTs directly without...
Abstract The activity of a kinesin is largely determined by the ~350 residue motor domain and this region alone sufficient to classify as member particular family. Kinesin-13 family are group microtubule depolymerising kinesins vital regulators length. Members critical spindle assembly chromosome segregation in both mitotic meiotic cell division play crucial roles cilium length control neuronal development. To better understand evolution depolymerisation family, we created synthetic...