A5018709460- Advanced Glycation End Products research
- Biofuel production and bioconversion
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Microbial Metabolic Engineering and Bioproduction
- Alzheimer's disease research and treatments
- Protein Hydrolysis and Bioactive Peptides
- Trypanosoma species research and implications
- Trace Elements in Health
- Eosinophilic Esophagitis
- Endoplasmic Reticulum Stress and Disease
- Research on Leishmaniasis Studies
- Gastroesophageal reflux and treatments
- Fungal and yeast genetics research
- Dysphagia Assessment and Management
- Food Quality and Safety Studies
- Biochemical Acid Research Studies
- Insect Pest Control Strategies
- Enzyme Catalysis and Immobilization
- Fermentation and Sensory Analysis
- Nitric Oxide and Endothelin Effects
- Esophageal and GI Pathology
- Biochemical effects in animals
- Metabolism and Genetic Disorders
- Horticultural and Viticultural Research
- Natural Antidiabetic Agents Studies
University of Lisbon
2006-2019
Universidade do Porto
2007-2011
Hospital Curry Cabral
2004
Universidade Estadual de Campinas (UNICAMP)
2003
Universidade da Coruña
1994
Hospital de Santa Maria
1994
Instituto de Investigação Científica Tropical
1990
Methylglyoxal metabolism was studied during Saccharomyces cerevisiae grown with D ‐glucose as the sole carbon and energy source. Using for first time a specific assay methylglyoxal in yeast, metabolic fluxes of its formation ‐lactate production were determined. ‐Glucose consumption ethanol determined growth. Metabolic also situ, at glycolytic triose phosphate levels glyoxalase pathway. Maximum glucose correspond to maxima is quantitatively related glycolysis, representing 0.3% total flux S. .
FAP (familial amyloidotic polyneuropathy) is a systemic amyloid disease characterized by the formation of extracellular deposits transthyretin. More than 80 single point mutations are associated with amyloidogenic behaviour and onset this fatal disease. It believed that mutant forms transthyretin lead to decreased stability tetramer, which dissociates into monomers prone unfolding aggregation, later forming β-fibrils in deposits. This theory does not explain nor why they toxic nearby cells....
Protein glycation by methylglyoxal is a nonenzymatic post-translational modification whereby arginine and lysine side chains form chemically heterogeneous group of advanced end-products. Methylglyoxal-derived end-products are involved in pathologies such as diabetes neurodegenerative diseases the amyloid type. As produced nonenzymatically from dihydroxyacetone phosphate d-glyceraldehyde 3-phosphate during glycolysis, its formation occurs all living cells. Understanding model systems will...
Protein glycation is involved in structure and stability changes that impair protein functionality, which associated with several human diseases, such as diabetes amyloidotic neuropathies (Alzheimer's disease, Parkinson's disease Andrade's syndrome). To understand the relationship of dysfunction, unfolding beta-fibre formation, numerous studies have been carried out vitro. All these previous experiments were conducted non-physiological or pseudo-physiological conditions bear little to no...
In metabolomics there is an ever-growing need for faster and more comprehensive analysis methods to cope with the increase of biological studies. Direct infusion Fourier-transform ion cyclotron-resonance mass spectrometry (DI-FTICR-MS) used in non-targeted obtain high-resolution snapshots metabolic state a system. any profiling study, establishment effective metabolite extraction protocol paramount. We developed improved method, compatible DI-FTICR-MS-based metabolomics, using grapevine...
Methylglyoxal is the most important intracellular glycation agent, formed nonenzymatically from triose phosphates during glycolysis in eukaryotic cells. Methylglyoxal‐derived advanced end‐products are involved neurodegenerative disorders (Alzheimer's, Parkinson's and familial amyloidotic polyneurophathy) clinical complications of diabetes. Research models for investigating protein its relationship to methylglyoxal metabolism required understand this process, implications cell biochemistry...
The glyoxalase pathway catalyzes the formation of d-lactate from methylglyoxal, a toxic byproduct glycolysis. In trypanosomatids, trypanothione replaces glutathione in this pathway, making it potential drug target, since its selective inhibition might increase methylglyoxal concentration parasites. Two II structures were solved. One with bound spermidine molecule (1.8 Å) and other at active site (1.9 Å). second structure was obtained by crystal soaking enzyme substrate...
Familial amyloidotic polyneuropathy (FAP) is a systemic conformational disease characterized by extracellular amyloid fibril formation from plasma transthyretin (TTR). This crippling, fatal for which liver transplantation the only effective therapy. More than 80 TTR point mutations are associated with diseases and most widely accepted model relates tetramer instability mutations. However, this fails to explain two observations. First, native also forms in senile amyloidosis, geriatric...
Transthyretin amyloidosis is a conformational pathology characterized by the extracellular formation of amyloid deposits and progressive impairment peripheral nervous system. Point mutations in this tetrameric plasma protein decrease its stability are linked to disease onset progression. Since non-mutated transthyretin also forms systemic senile some mutation bearers asymptomatic throughout their lives, non-genetic factors must be involved amyloidosis. We discovered, using differential...
The kinetics of glyoxalase I [( R ) ‐S‐ lactoylglutathione methylglyoxal‐lyase; EC 4.4.1.5] and II ( S ‐2‐hydroxyacylglutathione hydrolase; 3.1.2.6) from Saccharomyces cerevisiae was studied in situ , digitonin permeabilized cells, using two different approaches: initial rate analysis progress curves analysis. Initial performed by hyperbolic regression rates the program hyperfit . Glyoxalase exhibited saturation on 0.05–2.5 m hemithioacetal concentration range, with kinetic parameters K 0.53...
The Maillard reaction between reducing sugars and amino groups of biomolecules generates complex structures known as AGEs (advanced glycation endproducts). These have been linked to protein modifications found during aging, diabetes various amyloidoses. To investigate the contribution alternative routes formation AGEs, we developed a mathematical model that describes generation CML [ N(epsilon)-(carboxymethyl)lysine] in glucose collagen. Parameter values were obtained by fitting published...
The glyoxalase pathway of Leishmania infantum was kinetically characterized as a trypanothione‐dependent system. Using time course analysis based on parameter fitting with genetic algorithm, kinetic parameters were estimated for both enzymes, trypanothione derived substrates. A K m 0.253 and V 0.21 µmol·min −1 ·mg I, 0.098 0.18 II, obtained. Modelling computer simulation used evaluating the relevance potential therapeutic target by revealing importance critical this in . sensitivity...
Metabolism, the set of all chemical transformations inside a living cell, comprises nonenzymatic processes that generate toxic products such as reactive oxygen species and 2-oxoaldehydes. Methylglyoxal, highly 2-oxoaldehyde by-product glycolysis, is able to react irreversibly nonenzymatically with proteins, forming methylglyoxal advanced glycation end-products, which alter protein structure, stability function. Therefore, may influence cell metabolism its physiology in way beyond what can be...
European CEN Standard EN 15251 (2007) requires indoor thermal comfort conditions to be assessed and fall within a category system for different levels of expectation building purpose. The COMMONCENSE project has prepared provided information about the energy implications in particular whether is encouraging high–energy buildings case new major rehabilitations. Qualitative quantitative are given, using range cooling heating techniques forms. More specifically, required consumption twenty...