A5014397554- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- biodegradable polymer synthesis and properties
- Silk-based biomaterials and applications
- Osteoarthritis Treatment and Mechanisms
- Nerve injury and regeneration
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Spinal Cord Injury Research
- Seaweed-derived Bioactive Compounds
- Hydrogels: synthesis, properties, applications
- Additive Manufacturing and 3D Printing Technologies
- Orthopaedic implants and arthroplasty
- Knee injuries and reconstruction techniques
- Tendon Structure and Treatment
- Spine and Intervertebral Disc Pathology
- Polymer Nanocomposites and Properties
- Polysaccharides and Plant Cell Walls
- Polymer crystallization and properties
- Facial Trauma and Fracture Management
- Artificial Intelligence in Healthcare and Education
- Bone health and osteoporosis research
- Artificial Intelligence in Healthcare
Administração Regional de Saúde de Lisboa e Vale do Tejo
2024-2025
Polytechnic Institute of Viseu
2025
Lusíada University of Porto
2025
Stemmatters (Portugal)
2008-2024
Universidade de Brasília
2024
National Institute of Science and Technology in Regenerative Medicine
2020
University of Minho
2008-2017
Bioengineering Center
2017
National Center for Genetic Engineering and Biotechnology
2017
John Wiley & Sons (United States)
2017
Abstract Gellan gum is a polysaccharide manufactured by microbial fermentation of the Sphingomonas paucimobilis microorganism, being commonly used in food and pharmaceutical industry. It can be dissolved water, when heated mixed with mono or divalent cations, forms gel upon lowering temperature under mild conditions. In this work, gellan hydrogels were analyzed as cells supports context cartilage regeneration. hydrogel discs characterized terms mechanical structural properties....
Intervertebral disc (IVD) degeneration is a challenging clinical problem that urgently demands viable nucleus pulposus (NP) implant materials. The best suited biomaterial for NP regeneration has yet to be identified, but it believed biodegradable hydrogel-based materials are promising candidates. In this work, we have developed ionic- and photo-crosslinked methacrylated gellan gum (GG–MA) hydrogels used in acellular cellular tissue-engineering strategies the of IVDs. physicochemical...
Fibrous structures mimicking the morphology of natural extracellular matrix are considered promising scaffolds for tissue engineering. This work aims to develop a novel hierarchical starch-based scaffold. Such were obtained by combination starch-polycaprolactone micro- and polycaprolactone nano-motifs, respectively produced rapid prototyping (RP) electrospinning techniques. Scanning electron microscopy (SEM) micro-computed tomography analysis showed successful fabrication multilayer scaffold...
Tissue engineering provides unique opportunities for regenerating diseased or damaged tissues using cells obtained from tissue biopsies. engineered grafts can also be used as high fidelity models to probe cellular and molecular interactions underlying developmental processes. In this study, we co-cultured human umbilical vein endothelial (HUVECs) mesenchymal stem (MSCs) under various environmental conditions elicit synergistic leading the colocalized development of capillary-like bone-like...
Spinal cord injury (SCI) represents a significant health and social problem, therefore it is vital to develop novel strategies that can specifically target it. In this context, the objective of present work was new range three-dimensional (3D) tubular structures aimed at inducing regeneration within SCI sites. Up six different 3D were initially developed by rapid prototyping: bioplotting-based on biodegradable blend starch. These then further complemented injecting Gellan Gum,...
Abstract The ability to control the architecture and strength of a bone tissue engineering scaffold is critical achieve harmony between host tissue. Rapid prototyping (RP) technique applied satisfy this need create directly from scanned digitized image defect site. Design construction complex structures with different shapes sizes, at micro macro scale, fully interconnected pore structure appropriate mechanical properties are possible by using RP techniques. In study, was used for production...
Low back pain is one of the most reported medical conditions associated to intervertebral disc (IVD) degeneration. Nucleus pulposus (NP) often regarded as structure where IVD degeneration begins. Gellan gum (GG)-based hydrogels for acellular and cellular tissue engineering strategies have been developed finding applications NP substitutes. The innovative strategy based on reinforcement hydrogel matrix with biocompatible biodegradable GG microparticles (MPs), which are expected improve...
Human articular cartilage functions under a wide range of mechanical loads in synovial joints, where hydrostatic pressure (HP) is the prevalent actuating force. We hypothesized that formation engineered can be augmented by applying such physiologic stimuli to chondrogenic cells or stem cells, cultured hydrogels, using custom-designed HP bioreactors. To test this hypothesis, we investigated effects distinct regimens on vitro either human nasal chondrocytes (HNCs) adipose (hASCs) encapsulated...
Abstract Protein-based hydrogels with distinct conformations which enable encapsulation or differentiation of cells are great interest in 3D cancer research models. Conformational changes may cause macroscopic shifts the hydrogels, allowing for its use as biosensors and drug carriers. In depth knowledge on how conformational proteins affect cell fate tumor formation is required. Thus, this study reports an enzymatically crosslinked silk fibroin (SF) hydrogel system that can undergo intrinsic...