A5066592927- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Liver physiology and pathology
- Tissue Engineering and Regenerative Medicine
- Semiconductor materials and devices
- RNA Interference and Gene Delivery
- Dental Implant Techniques and Outcomes
- Collagen: Extraction and Characterization
- Silicon and Solar Cell Technologies
- Advancements in Semiconductor Devices and Circuit Design
- Dendrimers and Hyperbranched Polymers
- Electrospun Nanofibers in Biomedical Applications
- Muon and positron interactions and applications
- Orthopaedic implants and arthroplasty
- Hydrogels: synthesis, properties, applications
- Osteoarthritis Treatment and Mechanisms
- Cellular Mechanics and Interactions
- Silk-based biomaterials and applications
- Bone Metabolism and Diseases
- Bone and Dental Protein Studies
- Alkaline Phosphatase Research Studies
- Bone fractures and treatments
- Thin-Film Transistor Technologies
National Institute of Advanced Industrial Science and Technology
2009-2024
Ulvac (Japan)
2012
Osaka University
2008
Nara Medical University
2007
Hyogo Prefectural Amagasaki General Medical Center
2006
Rice Research Institute
2006
Tokyo University of the Arts
2003
The University of Tokyo
1990-2003
Waseda University
2003
Tokyo Women's Medical University
2000-2003
We developed a novel method to obtain designed shape cell sheets for tissue engineering. Shaping of were achieved by the use poly(N-isopropylacrylamide) (PIPAAm) and poly(N,N'-dimethylacrylamide) (PDMAAm) temperature-responsive adhesive nonadhesive domains, respectively. These polymers covalently grafted onto culture polystyrene (TCPS) dish surfaces electron beam irradiation with mask patterns. At 37 °C, human aortic endothelial cells (HAECs) attached, spread, proliferated make monolayer...
Abstract Confluent human aortic endothelial cells (HAECs) cultured on thermo‐responsive culture dish grafted with poly ( N ‐isopropylacrylamide) were recovered as a contiguous cell sheet. The double‐layered co‐culture was achieved by placing the HAEC sheet onto rat hepatocyte layer directly. structure of and hepatocytes remained in tight contact during culture. Hepatocytes layered system maintained differentiated shape albumin expression for over 41 days culture, whereas functions...
Abstract: Mesenchymal stem cells (MSCs) are multipotent and can be induced in vitro vivo to differentiate not only into the variety of mesodermal cells, but either ectodermal or endodermal cells. This capability indicates usefulness MSCs for tissue engineering. Cell surface antigen analyses using various types CD antibodies demonstrated that adherent fibroblastic derived from fresh human bone marrow mesenchymal showed extensive proliferation and/or differentiation. We labeled cultured as...
Acrylic acid (AAc) has been utilized to introduce reactive carboxyl groups a temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm). However, AAc introduction shifts the copolymer phase transition temperatures higher and dampens steep homopolymer with increasing content. We previously synthesized 2-carboxyisopropylacrylamide (CIPAAm) having both similar side chain structure IPAAm functional carboxylate group in order overcome these shortcomings. In present study, copolymers,...
Human bone marrow-derived mesenchymal cells contain stem (MSCs), which are well known for their osteo/chondrogenic potential and can be used reconstruction. This article reports the viability of cryopreserved human a comparison osteogenic between noncryopreserved with MSC-like characteristics, derived from marrow 28 subjects. The was approximately 90% regardless storage term (0.3 to 37 months). It is clear by fluorescence-activated cell sorter analysis that surface antigens both were...
It is well known that bone marrow contains mesenchymal stromal cells (MSCs), which can show osteoblastic differentiation when cultured in osteogenic medium containing ascorbic acid, beta-glycerophosphate and dexamethasone. The results the appearance of osteoblasts, together with formation matrix; thus, vitro (osteoblasts/bone matrix) could be fabricated by MSC culture. This type has already been used clinical cases involving orthopaedic problems. To improve therapeutic effect bone, we...
We evaluated the osteogenic differentiation of mesenchymal stem cells (MSCs) using a new class synthetic self-assembling peptide hydrogels, RADA 16, as scaffold for three-dimensional culture. MSCs derived from rat bone marrow were culture-expanded and seeded into hydrogel further cultured in medium containing beta-glycerophosphate, ascorbic acid, dexamethasone 2-4 weeks. High alkaline phosphatase activity osteocalcin (OC) contents detected at both protein gene expression levels during...
Abstract In this work, a new methodology is reported for developing hydroxyapatite (HA) scaffolds using an organic sacrifice template. The novelty of work consists possibility obtaining porous and highly interconnected mimicking the sacrificial component. Our purpose consisted evaluating physicochemical properties HA by means Fourier transform infra‐red spectroscopy, X‐ray diffraction analysis, scanning electron microscopy (SEM) attached with detector. obtained possess porosity ∼70%,...
Here we demonstrate a novel cell coculture method without any apparent limitation in cell-type combinations that exploits thermally responsive polymer-grafted patterns to alter cell–cell and cell–surface interactions. Thermally acrylamide polymer is first covalently patterned onto culture surfaces by masked electron beam irradiation. One type then cultured confluency at 37°C. Reducing temperature below 32°C selectively swells sensitive domains, detaching adherent cells only from these...
Marrow mesenchymal stem cells (MSCs) are multipotent progenitor and reported to be immunoprivileged as well immunosuppressive. Hence, MSCs might ideal candidates for allogeneic transplantation induce regeneration of damaged tissues/organs. To confirm the differentiation capability in vivo is important acceleration regenerative medicine. Consequently, we have established an rat model using subcutaneous implantation a hydroxyapatite (HA) ceramic/MSCs composite. Osteogenic was used indicator...
Abstract Novel highly branched biodegradable macromolecular systems have been developed by grafting carboxymethylchitosan (CMCht) onto low generation poly(amidoamine) (PAMAM) dendrimers. Such structures organize into sphere‐like nanoparticles that are proposed to be used as carriers deliver bioactive molecules aimed at controlling the behavior of stem cells, namely their proliferation and differentiation. The did not exhibit significant cytotoxicity in range concentrations below 1 mg mL −1 ,...
Abstract: Alumina ceramics have excellent mechanical and biocompatible properties, but are bioinert hence no bone‐bonding properties. We took a tissue‐engineering approach in an attempt to modify the ceramic surface so provide osteogenic/osteoconductive milieu. obtained human bone marrow mesenchymal cells from four donors then cultured for two weeks on alumina presence of β‐glycerophosphate, ascorbic acid dexamethasone. The showed extensive alkaline phosphatase staining mineralization, as...