A5049433541- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Polymer Surface Interaction Studies
- Hydrogels: synthesis, properties, applications
- Silk-based biomaterials and applications
- biodegradable polymer synthesis and properties
- Graphene and Nanomaterials Applications
- Nanocomposite Films for Food Packaging
- Spine and Intervertebral Disc Pathology
- Knee injuries and reconstruction techniques
- Osteoarthritis Treatment and Mechanisms
- Dental Implant Techniques and Outcomes
- Tendon Structure and Treatment
- Advanced Drug Delivery Systems
- Musculoskeletal pain and rehabilitation
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Phytochemistry and biological activity of medicinal plants
- Collagen: Extraction and Characterization
- Advanced Sensor and Energy Harvesting Materials
- Periodontal Regeneration and Treatments
- Graphene research and applications
- Polydiacetylene-based materials and applications
- Dental materials and restorations
- Total Knee Arthroplasty Outcomes
Hospital Braga
2013-2023
Biologie Tissulaire et Ingénierie Thérapeutique
2021
University of Minho
2009-2018
University of Aveiro
2017
Materials Research Group (United States)
2015-2016
Centre National de la Recherche Scientifique
2013-2015
Institut Néel
2015
Laboratoire des Matériaux et du Génie Physique
2012-2014
Institut polytechnique de Grenoble
2013
Institute for Biotechnology and Bioengineering
2008-2011
In this work, carbon nanofibers were used as doping material to develop a highly conductive chitosan-based composite. Scaffolds based on chitosan only and chitosan/carbon composites prepared by precipitation. Carbon homogeneously dispersed throughout the matrix, composite scaffold was porous with fully interconnected pores. Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar that measured for rat myocardium, excellent electrical properties, conductivity 0.25 0.09 S/m....
Abstract In this study, genipin‐cross‐linked collagen/chitosan biodegradable porous scaffolds were prepared for articular cartilage regeneration. The influence of chitosan amount and genipin concentration on the physicochemical properties was evaluated. morphologies characterized by scanning electron microscope (SEM) cross‐linking degree investigated ninhydrin assay. Additionally, mechanical assessed under dynamic compression. To study swelling ratio biostability scaffold, in vitro tests...
Injectable hyaluronic acid (HA)-based hydrogels compose a promising class of materials for tissue engineering and regenerative medicine applications. However, their limited mechanical properties restrict the potential range application. In this study, cellulose nanocrystals (CNCs) were employed as nanofillers in fully biobased strategy production reinforced HA nanocomposite hydrogels. Herein we report development new injectable composed adipic dihydrazide-modified (ADH-HA) aldehyde-modified...
Intervertebral disc (IVD) degeneration is a challenging clinical problem that urgently demands viable nucleus pulposus (NP) implant materials. The best suited biomaterial for NP regeneration has yet to be identified, but it believed biodegradable hydrogel-based materials are promising candidates. In this work, we have developed ionic- and photo-crosslinked methacrylated gellan gum (GG–MA) hydrogels used in acellular cellular tissue-engineering strategies the of IVDs. physicochemical...
Free-standing films have increasing applications in the biomedical field as drug delivery systems for wound healing and tissue engineering. Here, we prepared free-standing membranes by layer-by-layer assembly of chitosan alginate, two widely used biomaterials. Our aim was to produce a thick membrane study permeation model drugs adhesion muscle cells. We first defined optimal growth conditions terms pH alginate concentration. The could be easily detached from polystyrene or polypropylene...
Due to the limited self-repair capacity of cartilage, regenerative medicine therapies for treatment cartilage defects must use a significant amount cells, preferably applied using hydrogel system that can promise their delivery and functionality at specific site. This paper discusses potential κ-carrageenan hydrogels stem cells obtained from adipose tissue in defects. The developed were produced by an ionotropic gelation method human (hASCs) encapsulated 1.5% w/v solution cell density 5 ×...
Natural polymers are adequate renewable resources for the processability of well-defined architectures several applications. Combinations polysaccharides and proteins may mimic naturally occurring environment certain tissues. The main goal this work renders application green chemistry principles, namely use ionic liquids (ILs) biorenewable sources, such as chitosan (CHT) silk fibroin (SF), to process new hydrogel-based constructs. Although solubilization both materials in ILs has been...
Tissue engineering strategies have been showing promising early results in articular cartilage lesions repair. Hydrogels based on natural origin polymers as chitosan glycerol-phosphate (CGP) thermo-sensitive formulation that can be implanted a minimal invasive manner, represent great promise injectable scaffold choice for tissue engineering, but it lacks mechanical properties. A different formulation, from which firm texture gels is, therefore, desirable.
Low back pain is one of the most reported medical conditions associated to intervertebral disc (IVD) degeneration. Nucleus pulposus (NP) often regarded as structure where IVD degeneration begins. Gellan gum (GG)-based hydrogels for acellular and cellular tissue engineering strategies have been developed finding applications NP substitutes. The innovative strategy based on reinforcement hydrogel matrix with biocompatible biodegradable GG microparticles (MPs), which are expected improve...
To overcome current limitations of Tissue Engineering (TE) strategies, deeper comprehension on meniscus biology is required. This study aims to combine biomechanical segmental analysis fresh human tissues and its correlation with architectural cellular characterization.Morphologically intact menisci, from 44 live donors were studied after division into three radial segments. Dynamic mechanical (DMA) was performed at physiological-like conditions. Micro-computed tomography (CT) freeze-dried...
Engineering metabolically demanding tissues requires the supply of nutrients, oxygen, and removal metabolic byproducts, as well adequate mechanical properties. In this work, we propose development chitosan (CHIT)/alginate (ALG) freestanding membranes fabricated by layer-by-layer (LbL) assembly. CHIT/ALG were cross-linked with genipin at a concentration 1 mg·mL–1 or 5 mg·mL–1. Mass transport properties glucose oxygen evaluated on membranes. The diffusion decreases increasing cross-linking...
We investigated the pH-dependent properties of multilayered films made chitosan (CHI) and alginate (ALG) focused on their postassembly response to different pH environments using a quartz crystal microbalance with dissipation monitoring (QCM-D), swelling studies, ζ potential measurements, dynamic mechanical analysis (DMA). In an acidic environment, multilayers presented lower values and, consequently, higher moduli when compared obtained for used during assembly (5.5). When were exposed...
The lack of knowledge on the degradation layer-by-layer structures is one causes hindering its translation to preclinical assays. enzymatic chitosan/hyaluronic acid films in form ultrathin films, freestanding membranes, and microcapsules was studied resorting hyaluronidase. reduction thickness dependent hyaluronidase concentration, leading topography variations. Freestanding membranes exhibited accelerated weight loss up 120 h presence enzyme, achieving complete degradation. Microcapsules...
The development of novel silk/nano-sized calcium phosphate (silk/nano-CaP) scaffolds with highly dispersed CaP nanoparticles in the silk fibroin (SF) matrix for bone tissue engineering.Nano-CaP was incorporated a concentrated aqueous SF solution (16 wt.%) by using an situ synthesis method. silk/nano-CaP were then prepared through combination salt-leaching/lyophilization approaches.The particles presented good affinity to and their size inferior 200 nm when theoretical CaP/silk ratios between...
The regeneration of soft biological tissues requires new substitutes that exhibit mechanical properties similar to the native tissue. Herein, thin saloplastic membranes with tunable physical are prepared by complexation chitosan and alginate solutions containing different concentrations sodium chloride. Polyelectrolyte complexes (PECs) transferred flat Petri dishes for compaction into membrane shapes sedimentation solvent evaporation. All resistant degradation lysozyme stable in pH values...