A5102865682- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Ovarian function and disorders
- Birth, Development, and Health
- Reproductive Physiology in Livestock
- Calcium signaling and nucleotide metabolism
- Pregnancy and preeclampsia studies
- Cytomegalovirus and herpesvirus research
- Reproductive Health and Technologies
- Genetic and phenotypic traits in livestock
- Animal Behavior and Welfare Studies
- Virus-based gene therapy research
- Renal and related cancers
- Assisted Reproductive Technology and Twin Pregnancy
Universidad de Murcia
2020-2025
Instituto Murciano de Investigación Biosanitaria
2020-2023
Assisted reproductive technologies (ART) are routinely used in livestock to generate animals of high genetic value. Despite representing an outstanding accomplishment, recent studies suggest differences health, fertility, and gestational length vitro-produced compared vivo-derived animals. Currently, there no data available on the long-term effects ART growth development. This observational study aimed understand relationship between growth-influencing hormones a herd cattle derived from...
Studies of knockout (KO) mice with defects in the endolysosomal two-pore channels (TPCs) have shown TPCs to be involved pathophysiological processes, including heart and muscle function, metabolism, immunity, cancer, viral infection. With objective studying TPC2's roles for first time a large, more humanlike animal model, TPC2 KO pigs were produced using CRISPR-Cas9. A major problem CRISPR-Cas9 edit embryos is mosaicism; thus, we studied effect microinjection timing on mosaicism. Mosaicism...
This work was designed to determine temperature conditions within the reproductive tract of female pig and study their impact on ARTs. Temperatures were recorded using a laparo-endoscopic single-site surgery assisted approach miniaturized probe. Sows gilts used address natural cycle ovarian stimulation treatments, respectively. According in vivo values, IVF performed at three (37.0°C, 38.5°C 39.5°C) presumptive zygotes cultured these for 20 h, while further embryo culture (EC) (21-168 h...
Mosaicism is the most important limitation for one-step gene editing in embryos by CRISPR/Cas9 because cuts and repairs sometimes take place after first DNA replication of zygote. To try to minimize risk mosaicism, this study a reversible inhibitor was used release cell. There no previous information on use aphidicolin porcine embryos, so inhibition effect embryo development different concentrations drug evaluated. The incubation with tested at delivery methodologies. As result, observed, it...
Limb girdle muscular dystrophy type R1 (LGMDR1) is an autosomal recessive myopathy described in humans resulting from a deficiency of calpain-3 protein (CAPN3). This disease lacks effective treatment and appropriate model, so the generation KO pigs by CRISPR-Cas9 offers way to better understand ethology develop novel therapies. Microinjection main method for gene editing porcine embryo, but electroporation, which allows handling more embryos faster easier, has also recently been reported....
More suitable and efficient methods to protect gametes from external harmful effects during in vitro handling can be achieved by adding preovulatory porcine oviductal fluid (pOF) culture media. The objective of this study was assess the swim-up procedure’s suitability as a sperm selection method using medium supplemented with 1mg/mL BSA, 1% pOF (v/v), v/v plus 5mg/mL BSA. After selection, various parameters were studied, such recovery rate, morphology, motility (by CASA), vitality, acrosome...
The in vitro production (IVP) and subsequent transfer of embryos (ET) to recipient mothers is not yet an established reproductive technology the pig industry, as it cattle. However, that trade IVP-cryopreserved expected start next decades. Society governments are increasingly aware repercussions IVP could have for animal health, welfare, behavior, or food safety, but proven scientific information this type does exist, since no colonies pigs been created end. We a small one studied...
Animals born from in-vitro-produced (IVP) embryos show changes in the placenta and umbilical cord vascularization. This study compares placental vascular morphometry pigs (n = 19) through artificial insemination (AI group) or after transfer of IVP cultured with (RF-IVP without (C-IVP reproductive fluids. The relationship between parameters animal growth during first year life was also analyzed. Samples were collected at birth, fixed, paraffin-embedded, cut sections, stained, photographed for...
Abstract We have recently described a new model to study gamete interaction in mammalian species. The recreates the spherical surface of oocyte by using magnetic Sepharose beads coated with layer recombinant protein involved (such as ZP2, or IZUMO1 receptor JUNO) and an external cumulus oophorus cells, thus mimicking, some extent, native cumulus‐oocyte complex. Once generated, this 3D can be used sperm‐binding assay obtain valuable information about molecular basis interaction, since...