Abstract Introduction Poor ovarian reserve (POI) is one of the main factors that affect women with fertility problems. In this study, we tested whether making small incisions in cortex leads to changes biomechanics ovary could result reactivation functionality and folliculogenesis. Methods Given its similarity female reproductive system, both anatomically physiologically, used 5-year-old sheep (n=3) as an animal model. Incisions were made ovaries (OD) by laparoscopy, other left control (OC)....

The semen of boar is characterized by ejaculation in well-differentiated fractions with specific concentration, composition, and volume. 'sperm-rich fraction' (SRF), the most concentrated seminal fraction, habitually collected insemination centers to make artificial (AI) doses. absence other AI doses could alter uterine reaction not trigger essential responses that maximize fertility. Thus, there an urge ascertain impact different ejaculate on uterus after optimize This work analyzed...

Abstract The most commonly used influenza vaccines are made from inactivated viruses and administered via the intramuscular route. Although these can protect severe lower respiratory tract disease, they do not completely prevent virus replication in upper tract, this may lead to excretion dissemination. Therefore, nasally live-attenuated (LAIV) that induce mucosal immunity have been developed, but finding an optimal balance between sufficient attenuation immunogenicity remained challenging....

The available E. coli vaccines involve two main types (inactivated and live non-pathogenic) routes of administration (oral parenteral) but the mechanism by which both work is not yet fully elucidated. influence a parenteral vaccine (PV) an oral one (OV) was studied analyzing gene expression biomarkers indicating cellular infiltration (calprotectin, CAL), tight junction proteins (occludin OCL, zonulin ZON) that maintain intestinal paracellular integration proinflammatory (IFN-γ)...

Intra-abdominal hypertension and abdominal compartment syndrome are common with clinically significant consequences. We investigated the pathophysiological effects of raised IAP as part a more extensive exploratory animal study. The study design included both pneumoperitoneum mechanical intestinal obstruction models.Forty-nine female swine were divided into six groups: control group (Cr; n = 5), three groups IAPs 20mmHg (Pn20; 10), 30mmHg (Pn30; 40mmHg (Pn40; two occlusion (MIO20; 9) (MIO30;...

A mechanical intestinal obstruction (MIO) can generate intraabdominal hypertension (IAH) that is life threatening. The intestines are very sensitive to IAH since the low splanchnic perfusion causes hypoxia, local acidosis and bacterial translocations. This may lead acute distress syndrome (AIDS). identification of injuries during its correlation with clinical parameters as abdominal pressure (APP), gastric intramucosal pH (pHi) lactic acid (Lc) still unknown. study aimed evaluate sequence...

A bovine herd with a high prevalence of paratuberculosis (PTB) cohabiting population pigeons was studied (2011−2020). After finding the disease in 2011, annual monitoring performed 2012−2014 by obtaining blood samples for ELISA and intradermal tuberculinization (IT) tests Mycobacterium avium subsp. (MAP). Positive animals were eliminated. PTB dropped from 10% to 0% but returned similar values (9.5%) after 6 years without tests. In all animals, Ac according optical density (OD) determined...

Abstract Introduction Premature ovarian failure is one of the main problems presented by women with fertility problems. This pathology characterized a low reserve or presence quiescent follicles that for unknown reasons have not been activated and able to carry out their recruitment subsequent maturation. Recent studies suggest activation in state capable being rescued function as possible therapeutic route. Based on this hypothesis, objective our study was verify whether through surgical...

Live-attenuated influenza vaccines (LAIV) offer advantages over the commonly used inactivated split vaccines. However, finding optimal balance between sufficient attenuation and immunogenicity has remained a challenge. We recently developed an alternative LAIV based on 2009 pandemic H1N1 virus with truncated NS1 protein lacking PA-X expression (NS1(1-126)-ΔPAX). This showed blunted replication elicited strong innate immune response. In present study, we evaluated efficacy of this vaccine...

Post-weaning stress, together with Escherichia coli, are two of the key factors in occurrence post-weaning diarrhea. There different commercial vaccines that induce immunity at local or systemic level, improving farm health and avoiding economic losses pork industry. That is why objective this study was to evaluate effect an oral enterotoxigenic E. coli F4/F18 vaccine on intestinal integrity middle long term after inoculation. The gene expression biomarkers indicative cellular infiltration...

Abstract This study describes a case of 20‐day‐old male fighting bull with bilateral clinical anophthalmia and brachygnathia superior whose dam was 12.5 years old mistakenly dewormed ivermectin intramuscularly in the first third gestation livestock farm. A macroscopic examination carcass performed, special focus on ocular components. Eyeball remains were found both orbits histopathological performed them. Antibodies by serological against bovine herpes virus‐1, respiratory syncytial virus...

Animals born from in-vitro-produced (IVP) embryos show changes in the placenta and umbilical cord vascularization. This study compares placental vascular morphometry pigs (n = 19) through artificial insemination (AI group) or after transfer of IVP cultured with (RF-IVP without (C-IVP reproductive fluids. The relationship between parameters animal growth during first year life was also analyzed. Samples were collected at birth, fixed, paraffin-embedded, cut sections, stained, photographed for...

Oxygen (O2 ) rates in the oviduct are essential to human and animal reproduction. These regulated by activity of hypoxia markers such as hypoxia-inducible factors (HIFs), glucose transporters (GLUT), carbonic anhydrase (CA). In porcine model, scarce studies have been reported regarding these their effects reproduction unknown. The objective was characterize immunolocalization HIF-2α, GLUT1, CAIX oviducts throughout estrous cycle. Oviducts (ampulla isthmus) adult sows (n = 45) were collected...

Animals born from in-vitro produced (IVP) embryos show changes in the placenta and umbilical cord vascularization. This study compares placental vascular morphometry pigs (n=19) through artificial insemination (AI group) or after transfer of IVP cultured with (RF-IVP without (C-IVP reproductive fluids. The relationship between parameters animal growth during first year life was also analyzed. Samples were collected at birth, fixed, paraffin-embedded, cut sections, stained, photographed for...

Some regions of Spain have control and eradication programs to fight against goat tuberculosis. These official are based on a type diagnosis that relays cellular immunity (Intra dermal tuberculin test IFN-γ release assay), however, there is humoral (serology) could be useful as an ancillary for the detection animals with predominant response. Those goats related more advanced phase disease they can spread bacteria in easier way causing persistent infection some farms. Since these tests...