A5021747794- Reproductive Biology and Fertility
- Ovarian function and disorders
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Reproductive Physiology in Livestock
- Reproductive Health and Technologies
- Reproductive System and Pregnancy
- Genetic and phenotypic traits in livestock
- Assisted Reproductive Technology and Twin Pregnancy
- Cytomegalovirus and herpesvirus research
- Animal Behavior and Welfare Studies
- Xenotransplantation and immune response
- Calcium signaling and nucleotide metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Pluripotent Stem Cells Research
Universidad de Murcia
2019-2025
Instituto Murciano de Investigación Biosanitaria
2019-2022
Studies of knockout (KO) mice with defects in the endolysosomal two-pore channels (TPCs) have shown TPCs to be involved pathophysiological processes, including heart and muscle function, metabolism, immunity, cancer, viral infection. With objective studying TPC2's roles for first time a large, more humanlike animal model, TPC2 KO pigs were produced using CRISPR-Cas9. A major problem CRISPR-Cas9 edit embryos is mosaicism; thus, we studied effect microinjection timing on mosaicism. Mosaicism...
Abstract The addition of reproductive fluids (RF) to the culture media has shown benefits in different embryonic traits but its long-term effects on offspring phenotype are still unknown. We aimed describe such pigs. Blood samples and growth parameters were collected from piglets derived vitro-produced embryos (IVP) with or without RF added versus those artificially inseminated (AI), day 0 month 6 life. An oral glucose tolerance test was performed 45 show here first comparative data animals...
Abstract Background Molecules from the female reproductive tract modulate capacitation and function of sperm cells in vivo. These molecules vary a quantitative qualitative manner throughout estrous cycle. Objectives This work evaluates effect using various fluids on fertilization pig spermatozoa vitro. Material methods The effects culturing different levels protein kinase A (pPKA), tyrosine phosphorylation, acrosome reaction, vitro (IVF) were evaluated. tested as follows: oviductal fluid...
Assisted reproductive technologies play a major role in the cattle industry. An increase use of vitro -derived embryos is currently being seen around globe. But efficiency and quality are substandard when compared to vivo production. Different protocols have been designed overcome this issue, one those fluids as supplementation embryo culture media. In study, calves produced with added their production protocol were followed for first year life pairwise control, by artificial insemination...
Culture media supplemented with reproductive fluids (RF) have been used in livestock species, improving the efficiency and quality of vitro produced embryos. However, usefulness humans is still unknown. In this study, we collected human (HRFs) ex vivo (from 25 patients undergoing abdominal hysterectomy plus bilateral salpingectomy) 31 oocyte donors). Afterward, protocols to evaluate their osmolality, pH, total protein concentration, endotoxin level, sterility were optimized, establishing...
Assisted reproductive technologies (ART), besides solving several problems, it has also been used as a tool to improve the animal productivity that is required for feeding human population. One of these techniques, embryo transfer (ET), presented limitations in porcine species, which could constrain its use industry. To clarify potential this technique, we aimed compare impact using ET or artificial insemination (AI) on phenotype offspring during first days age, terms growth and blood...
More suitable and efficient methods to protect gametes from external harmful effects during in vitro handling can be achieved by adding preovulatory porcine oviductal fluid (pOF) culture media. The objective of this study was assess the swim-up procedure’s suitability as a sperm selection method using medium supplemented with 1mg/mL BSA, 1% pOF (v/v), v/v plus 5mg/mL BSA. After selection, various parameters were studied, such recovery rate, morphology, motility (by CASA), vitality, acrosome...
Each year, tens of thousands people worldwide die end-stage organ failure due to the limited availability organs for use in transplantation. To meet this clinical demand, one last frontiers regenerative medicine is generation humanized pigs from pluripotent stem cells (PSCs) via blastocyst complementation. For this, organ-disabled pig models are needed. As endothelial (ECs) play a critical role xenotransplantation rejection every organ, we aimed produce hematoendothelial-disabled embryos...
Abstract Reproductive fluids have been recently proposed as the most adequate natural additive to complement chemically defined in vitro culture media different animal models, since they contain full range of molecules necessary for fertilization and development preimplantation embryos. However, reproductive potentially are problematic terms biosecurity reproducibility results, their availability is scarce. Therefore, we optimized protocols characterize physico-chemical functional properties...