A5047923110- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Pelvic floor disorders treatments
- Infant Nutrition and Health
- Menstrual Health and Disorders
- Enzyme Catalysis and Immobilization
- Galectins and Cancer Biology
- Proteins in Food Systems
- Urological Disorders and Treatments
- 3D Printing in Biomedical Research
- Preterm Birth and Chorioamnionitis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Reproductive Biology
Karolinska Institutet
2020-2024
Universidad de Murcia
2014-2021
Instituto Murciano de Investigación Biosanitaria
2018
Following the fertilization of an egg by a single sperm, coat or zona pellucida (ZP) hardens and polyspermy is irreversibly blocked. These events are associated with cleavage N-terminal region (NTR) glycoprotein ZP2, major subunit ZP filaments. ZP2 processing thought to inactivate sperm binding ZP, but its molecular consequences connection hardening unknown. Biochemical structural studies show that triggers oligomerization. Moreover, structure native vertebrate filament, combined AlphaFold...
OVGP1 is the major non-serum glycoprotein in oviduct fluid at time of fertilization and early embryo development. Its activity differs among species. Here, we show that C-terminal region recombinant regulates its binding to extracellular zona pellucida affects during fertilization. While porcine penetrates two-thirds thickness pellucida, shorter glycoproteins, including rabbit OVGP1, are restricted outer one-third matrix. Deletion reduces ability penetrate through prevents endocytosis. This...
Previously, our group demonstrated that recombinant porcine oviductin (pOVGP1) binds to the zona pellucida (ZP) of in vitro-matured (IVM) oocytes with a positive effect on vitro fertilization (IVF). The fact pOVGP1 was detected inside IVM suggested this protein had biological role during embryo development. aim study evaluate effects bovine We applied 10 or 50 µg/ml IVF, embryonic culture (IVC), both, cleavage and Blastocyst quality assessed by analyzing expression important developmental...
Abstract The egg is a spherical cell encapsulated by the zona pellucida (ZP) which forms filamentous matrix composed of several glycoproteins that mediate gamete recognition at fertilization. Studies on molecular mechanisms sperm-egg binding are limited in many mammalian species scarcity eggs, ethical concerns harvesting and high cost producing genetically modified animals. To address these limitations, we have reproduced three-dimensional (3D) model mimicking oocyte’s shape, means magnetic...
Searchable abstracts of presentations at key conferences on reproductive biology and medicine ISSN 2052-1472 (online)