A5052715071- Medicinal plant effects and applications
- Acute Lymphoblastic Leukemia research
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Biological Activity of Diterpenoids and Biflavonoids
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Kawasaki Disease and Coronary Complications
- Natural product bioactivities and synthesis
- Immunotherapy and Immune Responses
- Genetic factors in colorectal cancer
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Blood disorders and treatments
- Acute Myeloid Leukemia Research
- Erythrocyte Function and Pathophysiology
- Medicinal Plant Pharmacodynamics Research
- Single-cell and spatial transcriptomics
- Inflammasome and immune disorders
- Chronic Lymphocytic Leukemia Research
- Advanced biosensing and bioanalysis techniques
- Lung Cancer Treatments and Mutations
Charles University
2020-2025
University Hospital in Motol
2022-2023
Opća bolnica Karlovac
2022
We compared minimal/measurable residual disease (MRD) levels evaluated by routinely used real-time quantitative polymerase chain reaction (qPCR) patient-specific assays and next-generation sequencing (NGS) approach in 780 immunoglobulin (IG) T-cell receptor (TR) markers 432 children with B-cell precursor acute lymphoblastic leukemia treated on the AIEOP-BFM ALL 2009 protocol. Our aim was to compare MRD-based risk stratification at end of induction. The results were concordant 639 (81.9%)...
The immunogenicity of the mRNA coronavirus disease 2019 vaccine in thoracic organ transplant recipients is poor.1,2 Early reports provided evidence increased after third dose solid recipients.3,4 However, antibody and cellular responses BNT162b2 (Pfizer-BioNTech) its safety lung (LTRs) are unknown to date. We included 15 LTRs without a history severe acute respiratory syndrome 2 (SARS-CoV-2) infection who received doses 21 d apart with no response. In this cohort, we assessed immediately...
Detection of measurable residual disease (MRD) in hematological malignancies is crucial for prognostication and treatment decisions.Real-time quantitative PCR (RQ-PCR), which targets immunoglobulin (IG) T-cell receptor (TR) rearrangements, deemed the gold-standard method MRD detection national international clinical trials.However, this approach does have some limitations, primarily related to standard curve performance non-specific background amplification.International guidelines criteria...
Abstract Monitoring of minimal residual disease (MRD) after hematopoietic stem cell transplantation (HSCT) in patients with acute lymphoblastic leukemia (ALL) is vital for timely therapeutic intervention planning. However, interpreting low‐positive results from the current standard method, quantitative PCR (qPCR) immunoglobulin and T‐cell receptor gene rearrangements (IG/TR), poses challenges due to risk false positivity caused by non‐specific amplification. We aimed improve MRD detection...
Anaplastic lymphoma kinase-positive anaplastic large cell (ALK+ ALCL) originates from the T-lineage and is marked by rearrangements of ALK gene. Over ten fusion partners with gene are known, most common being t(2;5)(p23;q35) translocation resulting in NPM1::ALK fusion. In 10-20% ALK+ ALCL cases, fuses various other partners. Modern molecular techniques, especially next-generation sequencing (NGS), have eased identification allowed in-depth characterization TCR repertoire. We devised a...
Abstract Background Multisystem inflammatory syndrome in children associated with COVID-19 (MIS-C) is a late complication of pediatric COVID-19, which follows weeks after the original SARS-CoV-2 infection, regardless its severity. It characterized by hyperinflammation, neutrophilia, lymphopenia, and activation T cells elevated IFN-γ. Observing production autoantibodies parallels systemic autoimmune disorders, such as lupus erythematodes (SLE), we explored B cell phenotype serum levels type...
Dihydromyricetin (DHM) is a natural flavonoid showing several health promoting effects such as protective activity during severe alcohol intoxication. The mechanism underlying the of DHM on metabolism virtually unknown. present paper focused clarifying role in liver elimination at its molecular level. First, impact dehydrogenase (ADH) vitro and enzyme induction vivo was examined. Neither ADH nor expression were influenced by DHM. Next, effect intoxication studied primary hepatocytes isolated...
Background : Common variable immunodeficiency (CVID) is characterized by an impaired post-vaccination response, high susceptibility to respiratory tract infections, and a broad spectrum of non-infectious complications. Thus, patients with CVID are at risk coronavirus disease 2019 (COVID-19), vaccination’s role in prevention questionable. The main aim this study was evaluate the clinical outcomes, safety, dynamics humoral T-cell immune responses induced mRNA vaccine BNT162b2 CVID. Methods...
Abstract Purpose: Anti-SARS-CoV-2 mRNA vaccines may elicit humoral responses in patients with primary antibody deficiency. However, this response is limited by its rapid waning and low levels of virus-neutralizing antibodies. There evidence for the efficacy booster vaccination deficiencies. Methods: This prospective observational cohort study focused on clinical outcomes (proportion infected patients, disease severity, adverse event incidence) dynamics immune (specific post-vaccination...
Abstract Multisystem Inflammatory Syndrome in Children associated with COVID-19 (MIS-C) is a late complication of pediatric COVID-19, which follows weeks after original SARS-CoV-2 infection, regardless its severity. It characterized by hyperinflammation, neutrophilia, lymphopenia and activation T cells elevated IFN-γ. Observing production autoantibodies parallels systemic autoimmune disorders, such as lupus erythematodes (SLE), we explored B cell phenotype serum levels type I, II III...