A5017163508- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Polyomavirus and related diseases
- Renal function and acid-base balance
- Tracheal and airway disorders
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Dialysis and Renal Disease Management
- SARS-CoV-2 and COVID-19 Research
- Organ and Tissue Transplantation Research
- Cystic Fibrosis Research Advances
- Pneumocystis jirovecii pneumonia detection and treatment
- Antifungal resistance and susceptibility
- Respiratory viral infections research
- Fungal Infections and Studies
- Microbial infections and disease research
- Venous Thromboembolism Diagnosis and Management
- Ion Transport and Channel Regulation
- Cytomegalovirus and herpesvirus research
- Child Nutrition and Feeding Issues
- COVID-19 Clinical Research Studies
- Viral-associated cancers and disorders
- Herpesvirus Infections and Treatments
- Electrolyte and hormonal disorders
- Mechanical Circulatory Support Devices
Charles University
2012-2025
University Hospital in Motol
2018-2025
University Health Network
2021-2024
University of Toronto
2023
B. Braun (Czechia)
2014-2018
The immunogenicity of the novel mRNA COVID-19 vaccine in immunocompromised lung transplant recipients is still unknown. We compared antibody response after first and second doses BNT162b2 (Pfizer-BioNTech) with natural SARS-CoV-2 infection recipients. None vaccinees tested two developed anti-SARS-CoV-2 IgG, while 85% patients presented an infection. absence to vaccination led us investigate cellular a subset patients. detected specific T-cells 4 out 12 Some therefore might have clinical...
Scoliotic deformity represents a serious spinal disorder that influences the locomotive and cardiopulmonary systems. Some patients with severe scoliosis end-stage lung disease are therefore denied transplantation. In considering transplantation, size match, straight back syndrome, delayed chest closure bronchial stenosis key issues clinicians should evaluate. Therefore, it is vital to determine donor-recipient matches very precisely. Chest opening routine intraoperative primary therapeutic...
The immunogenicity of the mRNA coronavirus disease 2019 vaccine in thoracic organ transplant recipients is poor.1,2 Early reports provided evidence increased after third dose solid recipients.3,4 However, antibody and cellular responses BNT162b2 (Pfizer-BioNTech) its safety lung (LTRs) are unknown to date. We included 15 LTRs without a history severe acute respiratory syndrome 2 (SARS-CoV-2) infection who received doses 21 d apart with no response. In this cohort, we assessed immediately...
Background There is limited data on optimal management of chronic lung allograft dysfunction (CLAD). We aimed to describe the variability diagnostic and therapeutic practices in Europe. Methods A structured questionnaire was sent 71 centers 24 countries. Questions were related contemporary clinical for work-up, monitoring treatment CLAD. Number transplant (LTx) procedures, patients follow-up collected. Results Forty-four (62%) responded from 20 countries, representing 74% European activity....
Introduction Compared with traditional static ice storage, controlled hypothermic storage (CHS) at 4–10°C may attenuate cold-induced lung injury between procurement and implantation. In this study, we describe the first European transplant (LTx) experience a portable CHS device. Methods A prospective observational study was conducted of all consecutively performed LTx following (11 November 2022 31 January 2024) two high-volume centers. The LUNGguard device used for CHS. preservation...
Aim: The evaluation of the incidence symptomatic upper limb venous thrombosis (ULVT) associated with midline catheters in patients admitted to hospital. Methods: frequency ULVT diagnosed a group confirmed by sonographic examination hospitalised at Faculty Hospital over period 1 year. Results: Four hundred thirty-nine were inserted 430 (250 women and 180 men) during year 2015. Nine had two catheters. average age patient was 68 years (range: 19–96 years). median time catheter introduction into...
Transplanted lungs suffer worse outcomes than other organ transplants with many developing chronic lung allograft dysfunction (CLAD), diagnosed by physiologic changes. Histology of transbronchial biopsies (TBB) yields little insight, and the molecular basis CLAD is not defined. We hypothesized that gene expression in TBBs would reveal nature distinguish from changes due simply to time posttransplant. Whole-genome mRNA profiling was performed microarrays 498 prospectively collected INTERLUNG...
In lung transplantation, antibody-mediated rejection (AMR) diagnosed by ISHLT criteria is uncommon compared to other organs, and previous studies failed find molecular AMR (ABMR) in biopsies. However, understanding of ABMR has changed with the recognition that kidney transplants often DSA-negative associated NK cell transcripts. We therefore searched for a similar ABMR-like state transbronchial biopsies (TBBs) using gene expression microarray results from INTERLUNG study (#NCT02812290)....
Chronic lung allograft dysfunction (CLAD) is a major complication after transplantation that results from complex interplay of innate inflammatory and alloimmune factors, culminating in parenchymal and/or obliterative airway fibrosis. Excessive IL-17A signaling chronic inflammation have been recognized as key factors these pathological processes. Herein, we developed model repeated mouse minor alloantigen-mismatched single-lung transplantation. Repeated intratracheal LPS instillations...
Background Chronic lung allograft dysfunction (CLAD) is the principal cause of graft failure in transplant recipients and prognosis depends on CLAD phenotype. We used a machine learning computed tomography (CT) texture analysis tool at diagnosis for phenotyping prognostication compared with radiologist scoring. Methods This retrospective study included all adult first double patients (January 2010–December 2015) (censored December 2019) inspiratory CT near diagnosis. The quantified...
Background Aspiration is a known risk-factor for adverse outcomes post-lung transplantation. Airway bile acids are the gold-standard biomarker of aspiration – however they released into duodenum and likely reflect concurrent gastrointestinal dysmotility. Previous studies investigating total airway pepsin have found conflicting results on its relationship with These measured pepsinogen in airways. Certain pepsinogens constitutively expressed lungs while others, like A4 (PGA4), not. We sought...
Histologic evaluation of allograft biopsies after lung transplantation has several limitations, suggesting that molecular assessment using tissue transcriptomics could improve biopsy interpretation. This single-center, retrospective cohort study evaluated discrepancies between the histology transbronchial (TBBs) with no rejection (NR) and T-cell mediated (TCMR) by diagnosis. The accuracy diagnosis was assessed based on response to treatment. 54 TBBs from Prague Lung Transplant Program...
Although posaconazole tablets show relatively low variability in pharmacokinetics (PK), the proportion of patients achieving PK/PD target at approved uniform dose for both prophylaxis and therapy is not satisfactory. The aim this study was to develop a population PK model lung-transplant recipients propose covariate-based dosing optimization therapy. In prospective study, 80 concentrations obtained from 32 during therapeutic drug monitoring were analyzed using nonlinear mixed-effects...
Jugular vein thrombosis (JVT) is usually secondary to central venous catheterisation, distant malignancy, hypercoagulable state or ovarian hyperstimulation syndrome. Spontaneous internal JVT an extremely rare entity. A 61-year-old man presented with swelling of the right side his face. An ultrasonographic scan showed jugular vein. Clinical tests, including oncomarkers, X-ray, CT and abdominal ultrasonography, searching for were negative. The patient was heparinized immediately using LMWH...
The aim of this prospective study was to evaluate the pharmacokinetics ganciclovir in lung transplant recipients, explore its covariates, and propose an individualized dosing regimen. Ganciclovir administered according protocol a standardized intravenous dose 5 mg/kg twice daily. Serum concentrations were monitored as trough at 3 h after dosing. Individual pharmacokinetic parameters calculated two-compartmental model, while regression models used covariates. Optimal loading maintenance doses...
This study aimed to explore pharmacokinetics of voriconazole and its covariates in lung transplant recipients using population approach order propose dosing individualization. Data from routine therapeutic drug monitoring adult treated with oral were analysed a three-stage pharmacokinetic model nonlinear mixed-effects modelling. Monte Carlo simulations based on final used generate the theoretical distribution profiles at various regimens. A total 78 serum concentrations collected 40 patients...