A5040890688- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Esophageal Cancer Research and Treatment
- Gastric Cancer Management and Outcomes
- Renal Transplantation Outcomes and Treatments
- Esophageal and GI Pathology
- Mechanical Circulatory Support Devices
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Polyomavirus and related diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Radiomics and Machine Learning in Medical Imaging
- Respiratory viral infections research
- Cancer-related molecular mechanisms research
- Tracheal and airway disorders
- Cancer Genomics and Diagnostics
- Virus-based gene therapy research
- Mycobacterium research and diagnosis
- Cancer Cells and Metastasis
- Nutrition and Health in Aging
- MicroRNA in disease regulation
- Pneumocystis jirovecii pneumonia detection and treatment
- Organ and Tissue Transplantation Research
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
Toronto General Hospital
2016-2025
University Health Network
2016-2025
University of Toronto
2016-2025
Royal College of Surgeons in Ireland
2024
Princess Margaret Cancer Centre
2019-2024
Faculty of 1000 (United States)
2024
RELX Group (United States)
2024
Health Net
2021-2024
St Joseph's Health Centre
2016-2024
NeoGenomics (United States)
2024
More than 80% of donor lungs are potentially injured and therefore not considered suitable for transplantation. With the use normothermic ex vivo lung perfusion (EVLP), retrieved can be perfused in an circuit, providing opportunity to reassess its function before In this study, we examined feasibility transplanting high-risk that have undergone EVLP.In prospective, nonrandomized clinical trial, subjected high risk transplantation 4 hours EVLP. High-risk were defined by specific criteria,...
Treatment of damaged donor lungs with the cytokine interleukin-10 improves their function, allowing previously unacceptable organs to be used for transplantation.
Treatment of injured donor lungs ex vivo to accelerate organ recovery and ameliorate reperfusion injury could have a major impact in lung transplantation. We recently demonstrated feasible technique for prolonged (12 h) normothermic perfusion (EVLP). This study was performed examine the EVLP on ischemic injury. Pig were cold preserved Perfadex 12 h subsequently divided into two groups: static preservation (CSP) or at 37 degrees C with Steen solution further (total 24 preservation). Lungs...
The growing demand for suitable lungs transplantation drives the quest alternative strategies to expand donor pool. aim of this study is evaluate outcomes lung (LTx) with donation after circulatory determination death (DCDD) and impact selective ex vivo perfusion (EVLP). From 2007 2013, 673 LTx were performed, 62 (9.2%) them using DCDDs (seven bridged cases). Cases mechanical ventilation/extracorporeal life support excluded. 55 DCDDs, 28 (51%) underwent EVLP. Outcomes neurological (DNDD)...
The mortality rate for individuals on the wait list lung transplant is 15% to 25%, and still only 20% of lungs from multiorgan donors are used transplant. donor pool may be increased by assessing reconditioning high-risk extended criteria with ex vivo perfusion (EVLP), similar short-term outcomes.To assess long-term outcomes recipients treated EVLP.This retrospective cohort single-center study was conducted August 1, 2008, February 28, 2017, among 706 not undergoing EVLP 230 EVLP.Donor...
Acellular normothermic ex vivo lung perfusion (EVLP) is a novel method of donor preservation for transplantation. As cellular metabolism preserved during perfusion, it represents potential platform effective gene transduction in lungs. We hypothesized that vector-associated inflammation would be reduced delivery due to isolation from the host immune system response. compared with intratracheal an E1-, E3-deleted adenoviral vector encoding either green fluorescent protein (GFP) or...
Ex vivo normothermic lung perfusion (EVLP) is a novel platform and method developed to facilitate functional assessment implementation of advanced therapies for donor lungs prior transplantation. This study aimed determine the safety immunological benefits ex adenoviral human interleukin-10 (AdhIL-10) gene delivery prevent development primary graft dysfunction in large animal survival model. Pig were retrieved, preserved 6 h at 4°C, then randomly assigned four groups: (1) AdhIL-10 therapy:...
In Brief Objectives: To study the impact of ex vivo lung perfusion (EVLP) on cytokines, chemokines, and growth factors their correlation with graft performance either during or after transplantation. Background: EVLP is a modern technique that preserves lungs normothermia in metabolically active state. The identification biomarkers clinical can contribute to safe expansion donor pool. Methods: High-risk brain death donors cardiac underwent 4 6 hours EVLP. Using multiplex magnetic bead array...
Cold static preservation on ice (~4°C) remains the clinical standard of donor organ preservation. However, mitochondrial injury develops during prolonged storage, which limits extent time that organs can maintain viability. We explored feasibility lung storage at 10°C using a large animal model and investigated mechanisms related to protection. Functional assessments performed ex vivo perfusion demonstrated porcine lungs stored for 36 hours had lower airway pressures, higher compliances,...
BackgroundCold static preservation (CSP) at higher temperatures (10°C) has been recently shown as an optimal strategy up to 24-36h of preservation. Here, we hypothesized that alternating 10°C storage with cycles normothermic ex vivo lung perfusion (EVLP) would provide conditions for cellular "recharge", allowing multi-day preservation.MethodsDonor lungs from male Yorkshire pigs were preserved using CSP two 4h EVLP. After a total 3 days preservation, left transplant was performed followed by...
BACKGROUND: Lung transplantation is performed on a 24/7 schedule to minimize organ ischemic time. Recent preclinical studies demonstrated superior graft preservation at 10°C compared with storage in an ice cooler (gold standard). METHODS: In this prospective, multicenter, nonrandomized clinical trial, we studied transplants from donors overnight cross-clamp times (6:00 p.m. 4:00 a.m.) that had earliest allowed starting time of 6:00 a.m. Lungs meeting criteria for were retrieved, transported,...
ABSTRACT To develop an ideal blood clot imaging and targeting agent, a single-chain antibody (SCA) fragment based on fibrin-specific monoclonal antibody, MH-1, was constructed produced via secretion from Bacillus subtilis . Through systematic study involving series of B. strains, insufficient intracellular extracytoplasmic molecular chaperones high sensitivity to wall-bound protease (WprA) were believed be the major factors that lead poor production MH-1 SCA. Intracellular apparently act in...
Uncontrolled donation after cardiac death (uDCD) has the potential to ameliorate shortage of suitable lungs for transplant. To date, no lung transplant data from these donors are available North America. We describe successful use using a simple method in situ inflation so that organ can be protected warm ischemic injury. Forty-four were approached, and family consent was obtained 30 cases (68%). Of these, team evaluated 16 uDCDs on site, 14 considered pending ex vivo perfusion assessment....