A5046096686- Organ Transplantation Techniques and Outcomes
- Mitochondrial Function and Pathology
- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- Tryptophan and brain disorders
- Extracellular vesicles in disease
- Diet and metabolism studies
- Mechanical Circulatory Support Devices
- Tissue Engineering and Regenerative Medicine
- Chronic Disease Management Strategies
- Neurological Disease Mechanisms and Treatments
- Congenital heart defects research
- Diabetes Management and Education
- Lipid metabolism and disorders
- Cardiac Arrest and Resuscitation
- Traumatic Brain Injury and Neurovascular Disturbances
- S100 Proteins and Annexins
- SARS-CoV-2 and COVID-19 Research
- Apelin-related biomedical research
- Metabolism and Genetic Disorders
- Cardiac Fibrosis and Remodeling
- Respiratory Support and Mechanisms
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Advanced Biosensing Techniques and Applications
- Birth, Development, and Health
University of Toronto
2018-2025
Canada Research Chairs
2024
University of New Brunswick
2024
Toronto Rehabilitation Institute
2018
University Health Network
2018
Sunnybrook Health Science Centre
2018
Sunnybrook Research Institute
2018
Cold static preservation on ice (~4°C) remains the clinical standard of donor organ preservation. However, mitochondrial injury develops during prolonged storage, which limits extent time that organs can maintain viability. We explored feasibility lung storage at 10°C using a large animal model and investigated mechanisms related to protection. Functional assessments performed ex vivo perfusion demonstrated porcine lungs stored for 36 hours had lower airway pressures, higher compliances,...
Despite tremendous progress in the development of mature heart-on-a-chip models, human cell-based models myocardial inflammation are lacking. Here, we bioengineered a vascularized with circulating immune cells to model severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced myocarditis. We observed hallmarks disease (COVID-19)-induced inflammation, as presence augmented secretion proinflammatory cytokines, triggered progressive impairment contractile function, and altered...
BackgroundCold static preservation (CSP) at higher temperatures (10°C) has been recently shown as an optimal strategy up to 24-36h of preservation. Here, we hypothesized that alternating 10°C storage with cycles normothermic ex vivo lung perfusion (EVLP) would provide conditions for cellular "recharge", allowing multi-day preservation.MethodsDonor lungs from male Yorkshire pigs were preserved using CSP two 4h EVLP. After a total 3 days preservation, left transplant was performed followed by...
Abstract Mitochondrial transplantation and transfer are being explored as therapeutic options in acute chronic diseases to restore cellular function injured tissues. To limit potential immune responses rejection of donor mitochondria, current clinical applications have focused on delivery autologous mitochondria. We recently convened a Transplant Convergent Working Group (CWG), explore three key issues that translation: (1) storage (2) biomaterials enhance mitochondrial uptake, (3) dynamic...
Angiogenesis may play an important role in the pathophysiology of Alzheimer's disease (AD). Previous vitro and vivo studies suggest a link between angiogenesis cerebral blood flow (CBF) AD; however, this has never been studied clinically. In sample study participants with early AD (n = 15), serum vascular endothelial growth factor (VEGF), biomarker, was negatively associated regional CBF (rCBF) angular gyrus even after bootstrapping at repetition 5000 controlling for age, sex, diagnosis (β...
Bipolar disorder (BD) is increasingly recognized as a with both mitochondrial dysfunction and heightened inflammatory reactivity, yet their contribution to neuronal activity remains unclear. To address these gaps, this study utilizes iPSC-derived cerebral organoids (COs) from BD patients healthy controls model disease-specific metabolic in physiologically relevant system. COs exhibited impairment, dysregulated function, increased nod-leucine rich repeat pyrin domain containing protein 3...
Cardiovascular disease continues to take more human lives than all cancer combined, prompting the need for improved research models and treatment options. Despite a significant progress in development of mature heart-on-a-chip fibrosis cardiomyopathies starting from induced pluripotent stem cells (iPSCs), cell-based myocardial inflammation are lacking. Here, we bioengineered vascularized system with circulating immune model SARS-CoV-2-induced acute myocarditis. Briefly, observed hallmarks...
Abstract Background Circulating cell‐free mitochondrial DNA (ccf‐mtDNA), a measure of dysfunction, is emerging as potential biomarker for early Alzheimer’s disease (AD). Decreased levels cerebrospinal fluid ccf‐mtDNA concentrations have been detected in AD patients. Few studies investigated peripheral or with cognitive measures, much less evaluating the role physical activity within relationships. Considering hallmark episodic memory impairment AD, we examined association between serum and...
Mild cognitive impairment (MCI) is a prodromal stage in aging to possible progression Alzheimer's disease and related dementia (ADRD), where co-occurrence of major depressive disorder (MDD) accelerates the progression. Metabolic mitochondrial abnormalities ADRD other neurodegenerative disorders have been widely suggested, while dysfunction has associated with etiopathology both MCI MDD. Hence, investigation markers MCI, MDD, presence conditions warranted. In total, 332 older adult...
Abstract Background Angiopoietin‐2, which regulates endothelial permeability and angiogenesis functions, is emerging as a potential biomarker for early Alzheimer’s disease (AD). Elevated levels of cerebrospinal fluid angiopoietin‐2 concentrations have been found in AD may be associated with the breakdown blood‐brain barrier that can contribute to cognitive decline. There lack research on blood cognition AD. Hence, we examined association between serum global cognition. Method Participants...