A5103598851- Lanthanide and Transition Metal Complexes
- Nanoparticle-Based Drug Delivery
- Advanced MRI Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Nanocluster Synthesis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoplatforms for cancer theranostics
- Trace Elements in Health
- Radiopharmaceutical Chemistry and Applications
- MRI in cancer diagnosis
- Medical Imaging Techniques and Applications
- Iron oxide chemistry and applications
- Magnetism in coordination complexes
- Supramolecular Self-Assembly in Materials
- Magnetic Properties and Synthesis of Ferrites
- Iron Metabolism and Disorders
- Click Chemistry and Applications
- SARS-CoV-2 and COVID-19 Research
- Radioactive element chemistry and processing
- Heavy Metal Exposure and Toxicity
- Viral gastroenteritis research and epidemiology
- Graphene research and applications
- Carbon and Quantum Dots Applications
- Adrenal Hormones and Disorders
- Pharmacological Effects of Medicinal Plants
Michigan State University
2022-2025
Oklahoma State Department of Health
2024
Northwestern University
2011-2022
Centre for Biomedical Engineering and Physics
2017
Imaging Center
2015
IPM Institute of North America
2011-2014
Institute of Neurobiology
2014
Evanston Hospital
2012
Argonne National Laboratory
2006-2008
University of Wisconsin–Madison
2006
A Gd(III)-nanodiamond conjugate [Gd(III)-ND] was prepared and characterized, enabling detection of nanodiamonds by MR imaging. The Gd(III)-ND particles significantly reduced the T1 water protons with a per-Gd(III) relaxivity 58.82 ± 1.18 mM−1 s−1 at 1.5 T (60 MHz). This represents 10-fold increase compared to monomer Gd(III) complex (r1 = 5.42 0.20 s−1) is among highest relaxivities reported.
Dental enamel, a hierarchical material composed primarily of hydroxylapatite nanowires, is susceptible to degradation by plaque biofilm-derived acids. The solubility enamel strongly depends on the presence Mg(2+), F(-), and CO3(2-). However, determining distribution these minor ions challenging. We show—using atom probe tomography, x-ray absorption spectroscopy, correlative techniques—that in unpigmented rodent Mg(2+) predominantly present at grain boundaries as an intergranular phase...
Conjugate agent: A GdIII enriched DNA-AuNP conjugate for intracellular magnetic resonance and fluorescence imaging is reported. The agent exhibits high relaxivity per particle cell uptake properties that provide a means to image map small populations. Detailed facts of importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by the authors. Please note: publisher responsible content...
Ultrasmall (3, 4, 5, and 6 nm), water-soluble Fe
Gadolinium(III) nanoconjugate contrast agents (CAs) have distinct advantages over their small-molecule counterparts in magnetic resonance imaging. In addition to increased Gd(III) payload, a significant improvement proton relaxation efficiency, or relaxivity (r1), is often observed. this work, we describe the synthesis and characterization of CA created by covalent attachment thiolated DNA (Gd(III)–DNA), followed surface conjugation onto gold nanostars (DNA–Gd@stars). These conjugates...
Multiple imaging modalities are often required for in vivo applications that require both high probe sensitivity and excellent spatial temporal resolution. In particular, MR optical an attractive combination can be used to determine molecular anatomical information. Herein, we describe the synthesis testing of two multimeric NIR–MR contrast agents contain three Gd(III) chelates IR-783 dye moiety. One agent contains a PEG linker other short alkyl linker. These label cells with extraordinary...
A one-pot reaction process was developed to synthesize highly dispersible, superparamagnetic Fe(3)O(4) nanoflowers; the potential of these nanoflowers as MRI contrast agents investigated.
Evidence suggests that chronic low level cadmium exposure impairs the function of insulin-producing β cells and may be associated with type-2 diabetes mellitus. Herein, we describe content in primary human islets define uptake kinetics effects environmentally relevant concentrations cultured cells. The average from 10 non-diabetic subjects was 29 ± 7 nmol/g protein (range to 72 protein). Exposure β-cell line MIN6 CdCl2 between 0.1 1.0 µmol/L resulted a dose- time-dependent over h. This an...
The unambiguous imaging of transplanted cells remains a major challenge to understand their biological function and therapeutic efficacy. In vivo implanted is reliant on tagging these differentiate them from host tissue, such as the brain. We here characterize gold nanoparticle conjugate that functionalized with modified deoxythymidine oligonucleotides bearing Gd(III) chelates red fluorescent Cy3 moiety visualize in human neural stem cells. This [email protected] ([email protected]) exhibits...
The ability to track labeled cancer cells in vivo would allow researchers study their distribution, growth, and metastatic potential within the intact organism. Magnetic resonance (MR) imaging is invaluable for tracking as it benefits from high spatial resolution absence of ionizing radiation. However, many MR contrast agents (CAs) required label either do not significantly accumulate or are biologically compatible translational studies. We have developed carbon-based...
Pancreatic adenocarcinoma has a 5 year survival of approximately 3% and median 6 months is among the most dismal prognoses in all medicine. This poor prognosis largely due to delayed diagnosis where patients remain asymptomatic until advanced disease present. Therefore, techniques allow early detection pancreatic are desperately needed. Imaging tissue notoriously difficult, development new imaging would impact our understanding organ physiology pathology with applications diagnosis, staging,...
Nanomaterials with mixed composition, in particular magnetic spinel ferrites, are emerging as efficient contrast agents for resonance imaging (MRI). Many factors, including size, atomic structure, and surface properties crucial the design of such nanoparticle-based probes due to their influence on properties. Silica-coated iron oxide (IO-SiO(2)) cobalt ferrite (CoIO-SiO(2)) nanoparticles were synthesized using standard high temperature thermal decomposition base-catalyzed water-in-oil...
The delivery of bioactive molecules into cells has broad applications in biology and medicine. Polymer-modified graphene oxide (GO) recently emerged as a de facto noncovalent vehicle for hydrophobic drugs. Here, we investigate different approach using native GO to deliver hydrophilic by co-incubation culture. adsorption were systematically studied with library 15 synthesized Gd(III) labels enable quantitation. Amines revealed be key chemical group adsorption, while was shown quantitatively...
Bioactive supramolecular nanostructures are of great importance in regenerative medicine and the development novel targeted therapies. In order to use chemistry design such nanostructures, it is extremely important track their fate vivo through molecular imaging strategies. Peptide amphiphiles (PAs) known generate a wide array there extensive literature on areas as tissue regeneration therapies for disease. We report here series PA molecules based well-established β-sheet amino acid sequence...
Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused global pandemic with millions infections hundreds thousands deaths date 1,2 . In response, we used rapid antibody discovery platform isolate human monoclonal antibodies (mAbs) against the spike (S) protein. We stratify these mAbs into five classes based on their reactivity subdomains S protein as well...
Autoimmune regulator (AIRE), a transcription factor expressed by medullary thymic epithelial cells, is required for shaping the self-antigen tolerant T cell receptor repertoire. Humans with mutations in AIRE suffer from Polyglandular Syndrome Type 1 (APS-1). Among many symptoms, men APS-1 commonly experience testicular insufficiency and infertility, but mechanisms causing infertility are unknown. Using an Aire -deficient mouse model, we demonstrate that male subfertility caused sterile...
Polymer-caged nanobins (PCNs) that can undergo Cu(I)-catalyzed click reactions enable the combination of GdIII magnetic resonance imaging (MRI) contrast agents and an anticancer drug (gemcitabine, GMC) into a single theranostic platform (see picture). The resulting gadolinium(III)-conjugated, GMC-loaded PCNs (GdIII–PCNGMC) exhibit significantly superior performance in r1 relaxivity, uptake, pH-sensitive release. Detailed facts importance to specialist readers are published as "Supporting...
Calcium [Ca(II)] is a fundamental transducer of electrical activity in the central nervous system (CNS). Influx Ca(II) into cytosol responsible for action potential initiation and propagation, initiates interneuronal communication via release neurotransmitters activation gene expression. Despite importance physiology, it remains challenge to visualize flux (CNS) vivo. To address these challenges, we have developed new generation, Ca(II)-activated MRI contrast agent that utilizes ethyl esters...
Magnetic resonance imaging (MRI) is a technique used in both clinical and experimental settings to produce high-resolution images of opaque organisms without ionizing radiation. Currently, MR augmented by contrast agents, the vast majority these small molecule Gd(III) chelates are confined extracellular regions. As result, agents vascular regions reducing their ability provide information about cell physiology or molecular pathology. We have shown that polypeptides arginine capacity...
A widely prevalent single nucleotide polymorphism, rs13266634 in the SLC30A8 gene encoding zinc transporter ZnT8, is associated with an increased risk for T2DM. ZnT8 mostly expressed pancreatic insulin-producing islets of Langerhans. The effect this variant on divalent metal profile human unknown. Additionally, essential and non-essential content under normal environmental exposure conditions has not been described. We therefore examined correlation other metals genotype demographic...
We report the structural optimization and mechanistic investigation of a series bioactivated magnetic resonance imaging contrast agents that transform from low relaxivity to high in presence Zn(II). The change results transformation complex alters coordination environment about Gd(III) center. Here, we have performed systematic modifications determine structure provides optimal response Relaxivity measurements absence Zn(II) were used conjunction with regarding water access (namely, number...
Progesterone receptor (PR) is strongly associated with disease prognosis and therapeutic efficacy in hormone-related diseases such as endometriosis breast, ovarian, uterine cancers. Receptor status currently determined by immunohistochemistry assays. However, noninvasive PR imaging agents could improve detection help elucidate pathological molecular pathways, leading to new therapies animal models. A series of water-soluble PR-targeted magnetic resonance (MRI) probes were synthesized using...
In vivo cell tracking is vital for understanding migrating populations, particularly cancer and immune cells. Magnetic resonance (MR) imaging long-term of transplanted cells in live organisms requires to effectively internalize Gd(III) contrast agents (CAs). Clinical Gd(III)-based CAs require high dosing concentrations extended incubation times cellular internalization. To combat this, we have devised a series Gd(III)-gold nanoconjugates (Gd@AuNPs) with varied chelate structure...