A5000608879- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Microbial Metabolic Engineering and Bioproduction
- RNA modifications and cancer
- Gene Regulatory Network Analysis
- Plant Gene Expression Analysis
- Microtubule and mitosis dynamics
- PARP inhibition in cancer therapy
- Plant nutrient uptake and metabolism
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
Charles University
2021-2024
Karlovac University of Applied Sciences
2023
ABSTRACT CSL proteins [named after the homologs CBF1 (RBP-Jκ in mice), Suppressor of Hairless and LAG-1] are conserved transcription factors found animals fungi. In fission yeast Schizosaccharomyces pombe, they regulate various cellular processes, including cell cycle progression, lipid metabolism adhesion. bind to DNA through their N-terminal Rel-like domain central β-trefoil domain. Here, we investigated importance binding for protein functions yeast. We created mutants with disrupted that...
N6-methyl adenosine (m6A) is a widespread internal mRNA modification impacting the expression of numerous genes. Here, we characterize auxin-related defects among pleiotropic phenotypes hypomorphic Arabidopsis thaliana mutants with impaired m6A status and reveal that they show strong resistance to exogenously applied auxin. By combining major published datasets, propose high-confidence target transcripts emerge those encoding main components required for auxin signaling, including TIR1/AFB...
Mitosis is a crucial stage in the cell cycle, controlled by vast network of regulators responding to multiple internal and external factors. The fission yeast Schizosaccharomyces pombe demonstrates catastrophic mitotic phenotypes due mutations or drug treatments. One factors provoking mitosis disturbed lipid metabolism, resulting from, for example, acetyl-CoA/biotin carboxylase (cut6), fatty acid synthase (fas2, also known as lsd1) transcriptional regulator metabolism (cbf11) genes, well...
ABSTRACT Chemical modifications of RNA affect essential properties transcripts, such as their translation, localization and stability. 5’-end capping with the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD + ) has been discovered in organisms ranging from bacteria to mammals. However, hypothesis that NAD might be universal all domains life not proven yet, information on this modification is missing for Archaea. Likewise, studied clinically important Mycobacterium genus....
Epithelial–mesenchymal transition (EMT) is a cellular mechanism used by cancer cells to acquire migratory and stemness properties. In this study, we show, through in vitro, vivo, 3D culture experiments, that the mitochondrial protein LACTB manifests tumor suppressor properties ovarian cancer. We show significantly down-regulated epithelial clinical tissues. Re-expression of negatively effects growth but not non-tumorigenic cells. Mechanistically, leads differentiation loss their properties,...
ABSTRACT Mitosis is a critical stage in the cell cycle, controlled by vast network of regulators responding to multiple internal and external factors. The fission yeast Schizosaccharomyces pombe may demonstrate catastrophic mitotic phenotypes due mutations or drug treatments. One factors provoking mitosis disturbed lipid metabolism, resulting from e.g. acetyl-CoA/biotin carboxylase ( cut6 ), fatty acid synthase fas2/lsd1 transcriptional regulator metabolism cbf11 ) genes, as well treatment...
ABSTRACT Within a eukaryotic cell, both lipid homeostasis and faithful cell cycle progression are meticulously orchestrated. The fission yeast Schizosaccharomyces pombe provides powerful platform to study the intricate regulatory mechanisms governing these fundamental processes. In S. , Cbf11 Mga2 proteins transcriptional activators of non-sterol metabolism genes, with also known as regulator. Despite sharing common set target little was about their functional relationship. This reveals that...
Within a eukaryotic cell, both lipid homeostasis and faithful cell cycle progression are meticulously orchestrated. The fission yeast Schizosaccharomyces pombe provides powerful platform to study the intricate regulatory mechanisms governing these fundamental processes. In S. pombe, Cbf11 Mga2 proteins transcriptional activators of non-sterol metabolism genes, with also known as regulator. Despite sharing common set target little was about their functional relationship. This reveals that...
ABSTRACT CSL (CBF1/RBP-Jκ/Suppressor of Hairless/LAG-1) proteins are conserved transcription factors found in animals and fungi. In fission yeast, they regulate various cellular processes, including cell cycle progression, lipid metabolism, adhesion. bind to DNA through their N-terminal Rel-like domain central beta-trefoil domain. Here, we investigated the importance binding for functions yeast Schizosaccharomyces pombe . We created mutants with disrupted that vast majority depend on intact...
In this study, we introduce a novel approach for the high-throughput quantitative analysis of mitotic defects in fission yeast Schizosaccharomyces pombe using Imaging Flow Cytometry (IFC). Fission is valuable model organism cell cycle research, and ‘cut’ phenotype, indicative catastrophe, has been instrumental discovering regulators. Traditional fluorescence microscopy methods quantifying events suffer from subjectivity limited throughput. Our IFC pipeline overcomes these limitations by...